Lithium−sulfur (Li−S) batteries are promising energy storage devices owing to their high theoretical specific capacity and energy density. However, several challenges, including volume expansion, slow reaction kinetics, polysulfide shuttle effect and lithium dendrite formation, hinder their commercialization. Separators are a key component of Li−S batteries. Traditional separators, made of polypropylene and polyethylene, have certain limitations that should be addressed. Therefore, this review discusses the basic properties and mechanisms of Li−S battery separators, focuses on preparing different functionalized separators to mitigate the shuttle effect of polysulfides. This review also introduces future research trends, emphasizing the potential of separator functionalization in advancing the Li−S battery technology.