JUNCAO technology plays a critical role in managing soil ecology and alleviating contradiction between mushroom and forest, as JUNCAO can partially replace the wood chip as mushroom culture medium. At present, few reports focus on exploring the effects of seeding density, nitrogen fertilizers on JUNCAO growth and their overwintering performance. To close the above-mentioned research gaps, five typical types of JUNCAO were evaluated by investigating their grass yield, overwintering germination rates and nutrient adsorption condition. The results indicated that Pennisetum sp. showed the best overwintering performance. In addition, the optimized planting conditions for Pennisetum sp. include cultivation density (60 cm × 50 cm), oblique seeding using stem with double nodes, and 800 kg·ha−1 nitrogen fertilizer. This study gave good insights into low-temperature resisting performance and their overwintering characteristics of diverse JUNCAO species that favor for promoting the safe and efficient productions of the JUNCAO industry in subtropical areas.