Status of cellular immunity in rats under conditions of acute widespread petitonitis in the setting of diabetes mellitus

Abstract

Acute generalized peritonitis (AGP) occurs in patients with concomitant pathology, in particular at diabetes mellitus. The severity of peritonitis depends on the adequacy of the immune response. Purpose of research was to study the features of cellular arm of immune response in the organism of experimental animals with simulated AGP in the setting of diabetes mellitus (DM). 56 white rats were used in the experiment, which was divided into three groups: main group – 24 animals with simulated AWP in the setting of DM; comparison group – 24 animals with simulated peritonitis. Animals of the main group AGP were modelled by injecting 10 % of filtered fecal suspension into the abdominal cavity of tested rats at a dose of 0,5 ml per 100 g of body weight. Removal of material for histological examination was performed on 1, 3 and 7 days. Cellular immunity was determined by a method based on the interaction of fluorescently labeed monoclonal antibodies with lymphocyte surface antigens. In both animals, all indicators of cellular immunity gradually decreased from day 1 to day 7 of the experiment, but these changes were more significant in animals with DM. With AGP, the cellular immune response imbalance is more significant in animals with DM than in animals with isolated AGP, which is characterized by a marked statistically significant decrease in the level of CD3+ cells, CD4+ cells, CD16+ cells, and a moderate increase in CD8+ cells. The imbalance of cellular immunity deepens depending on the duration of the lesion. The level of CD3+ cells at 1, 3, and 7 day in animals with AGP in the setting of DM was 53,77%, 60,48%, and 62,1 %, respectively, lower than the level in the group with animals with AGP. The prolonged imbalance of cellular immunity indices in experimental animals with simulated AGP in the setting of DM indicates not only the occurrence of secondary immunodeficiency, but also significant depletion of the body’s immune forces compared to animals with AGP.

The concept of the comorbidity model in predicting laparoscopic cholecystectomy results in patients with acute cholecystitis

“…Thus, the impact of comorbid pathology on clinical manifestations, diagnosis, prognosis, and treatment of many diseases is multifaceted and individual. The combination of diseases, age, and medical pathomorphism significantly changes the clinical picture and the course of the main nosology, in our case AC, the nature and severity of complications, worsens the patient's quality of life, limits or complicates the treatment and diagnostic process [ 5 – 8 ].…”

Імунний Статус І Динаміка Показників Cиндрому Ендогенної Інтоксикації При Експериментальному Гострому Поширеному Перитоніті На Тлі Стрептозотоциніндукованого Цукрового Діабету

“…Перебіг перитоніту залежить від стану імунної системи, адекватності імунної відповіді, яка є важливим критерієм у прогресуванні запального процесу в очеревині на тлі супутнього цукрового діабету. Ряд досліджень було спрямовано на вивчення клітинної та гуморальної ланок імунітету при ГПП [10][11][12][13]. Проте зв'язок між станом імунної системи та вираженням ендогенної інтоксикації при цій поєднаній патології, що являє собою багатокомпонентний загальний імунодепресивний стан, залишається недостатньо висвітленим, що і стало тригером дослідження.…”