Status of ESBL Producing Bacteria Isolated from Skin Wound at a Tertiary Care Hospital in Bangladesh

Yasmin, Taslima; Yusuf, Abdullah; Sayam, Mohammed Abdun Nur; Haque, Rezwana; Mowla, Golam

doi:10.4236/aid.2015.54022

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Notably, our results for most frequently isolated species, namely, S. aureus 23.7% and CoNS 15.8%, revealed to be different from those of other studies, where Klebsiella species (88.88%) were the most common ESBL producers from the skin wound infections, E. coli (61.5%) and Pseudomonas species (100.0%) (Yasmin et al, 2015).…”

Section: Discussioncontrasting

confidence: 99%

Prevalence of Extended Spectrum Beta Lactamase Producing Bacteria in Two Surgical Wards of a General Hospital

Zandersone

Medvedja

Jezupovs

et al. 2019

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Colonisation of gastrointestinal tract by extended spectrum beta lactamase (ESBL)-producing Gram-negative bacteria is a source for infections. The present work is a prospective study in Latvia aimed to determine the role of two surgical profile wards in transmission of ESBL-producing bacteria. Differences between hospital wards were not analysed due to low number of patients. We have also determined a correlation between the duration of hospitalisation and risk of ESBL colonisation. Tests for ESBL-producing bacteria were made twice for 136 patients — upon admission and upon discharge from the hospital. Of them, 21 (15.4%) patients already were ESBL-positive at the time of admission and 115 (84.6%) patients were ESBL-negative. Upon discharge from hospital, 45 (33.1%) patients were ESBL-positive, one patient was ESBL-negative, and 25 (18.4%) of ESBL-positive cases had emerged during hospitalisation. In total, 46 (33.8%) from 136 patients were ESBL-positive and ESBL was positive in 51 bacterial isolates. On discharge from hospital, the newly acquired ESBL-positive bacterial isolates were K. pneumoniae (n = 18), E. coli (n = 7) and P. mirabilis (n = 3). The prevalence of ESBL-positive E. coli from all detected E. coli was 7.0% and from all Klebsiella spp. — 88.9% in gut flora. Analysis of patientassociated wound infections did not show correlation between the ESBL-producing gut microbiota and the bacterial species involved in wound infection.

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Section: Discussioncontrasting

confidence: 99%

Prevalence of Extended Spectrum Beta Lactamase Producing Bacteria in Two Surgical Wards of a General Hospital

Zandersone

Medvedja

Jezupovs

et al. 2019

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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“…were highly resistant to tetracycline, rifampin, nalidixic acid, and 100% sensitive to imipenem. Yasmin et al (2015) [45] also found that Klebsiella isolates were amoxicillin and nalidixic acid resistant [46]. In contrast, we found the isolates of our study were mostly imipenem, meropenem and amikacin sensitive.…”

Section: Discussionsupporting

confidence: 39%

Prevalence and molecular characterization of antibiotic resistance and associated genes in Klebsiella pneumoniae isolates: A clinical observational study in different hospitals in Chattogram, Bangladesh

et al. 2021

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Objective This study was performed to investigate the prevalence of multidrug resistance and molecular characterization of Klebsiella pneumoniae (KPN) from clinical isolates in the southern region of Bangladesh. Additional analysis of the prevalence of blaNDM-1, blaSHV-11, uge genes of KPN was also carried out among these clinical isolates. Method The study was carried out using 1000 clinical isolates collected from two different hospitals of Chattogram. A drug susceptibility test was performed by the disk diffusion method to detect KPN’s response to 16 antibiotics. The presence of antibiotic-resistant and (or) virulent genes blaNDM-1, blaSHV-11, uge were investigated using the PCR technique. Isolates having blaNDM-1, blaSHV-11, uge gene were further validated by sequencing followed by phylogenetic analysis. Phylogenetic relationships among these isolates were determined by Clustal omega and MEGA7. Result A total of 79%, 77%, 74.9%, 71%, 66% and 65% isolates exhibited resistance against cefuroxime, cefixime, cefotaxime, ceftazidime, cefepime and ceftriaxone respectively. The frequency of resistance to other antibiotics varied from 26.5% to 61.8%. PCR analysis showed that 64% of strains harbored blaNDM-1 gene, and 38% strains harbored blaSHV-11 gene. Moreover, 47% of samples were carrying uge gene, and 19% of samples carried blaNDM-1, blaSHV-11, uge genes together. Conclusion In this study, we’ve analysed the pattern of expression as well as prevalence of blaNDM-1, blaSHV-11, and uge genes in Klebsiella isolates. Upon molecular and statistical analysis, we found a high prevalence of multi-drug resistance KPN strains in the isolates. The Klebsiella isolates were confirmed to harbor multiple ESBL genes and 64% of the isolates were found to be producing NDM-1. As multidrug resistance is an alarming issue, continuous surveillance and routine clinical detection of resistant bacteria and plasmids are necessary to prevent catastrophic public health incidents.

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Status of ESBL Producing Bacteria Isolated from Skin Wound at a Tertiary Care Hospital in Bangladesh

Cited by 2 publications

References 20 publications

Prevalence of Extended Spectrum Beta Lactamase Producing Bacteria in Two Surgical Wards of a General Hospital

Prevalence of Extended Spectrum Beta Lactamase Producing Bacteria in Two Surgical Wards of a General Hospital

Prevalence and molecular characterization of antibiotic resistance and associated genes in Klebsiella pneumoniae isolates: A clinical observational study in different hospitals in Chattogram, Bangladesh

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