Status of pyrethroid resistance and mechanisms in Brazilian populations of Tuta absoluta

Silva, Wellington Marcos da; Berger, Madeleine; Bass, Chris; Balbino, Valdir Queiroz; Amaral, Marcelo H.; Campos, Mateus Ribeiro de; Siqueira, Herbert A.A.

doi:10.1016/j.pestbp.2015.01.011

Cited by 50 publications

(39 citation statements)

References 39 publications

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“…The resistance ratios at LC 50 in comparison with the most susceptible population from Brazil (Campos et al., ) varied up to 12‐fold in resistance to chlorpyrifos. Deltamethrin leaf dip bioassays of the Zanjan and the Rasht populations showed 10‐fold and 13‐fold resistance, respectively, in comparison with the Tiangua population from Brazil (Silva et al., ). The comparisons of bioassay results between different laboratories are difficult even when the same methodology is used.…”

Section: Discussionmentioning

confidence: 99%

“…Silva et al. indicated no significant differences between β‐esterase and small differences in α‐esterase in Brazilian population of T. absoluta (Silva et al., ). Our study showed significant correlation between both esterase activities and the P450 activity to cypermethrin and deltamethrin.…”

Section: Discussionmentioning

confidence: 99%

“…Insecticide resistance in the tomato leaf miner has been reported in several countries against many insecticides from different chemical classes (Lietti, Botto, & Alzogaray, ; Roditakis, Papachristos, & Roditakis, ; Roditakis et al., ; Silva et al., ; Siqueira et al., ). Pyrethroid resistance in T. absoluta has been reported to be associated with the presence of the mutations L1014F, M918T and T929I in the sodium channel (Silva et al., ). Mutations were found in high frequencies in field strains from Europe and South America.…”

Section: Introductionmentioning

confidence: 99%

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Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran

Zibaee

Mahmood

Esmaeily

et al. 2017

J Applied Entomology

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The tomato leafminer, Tuta absoluta (Meyrich) (Lepidoptera: Gelechiidae), is a serious pest of tomato crops worldwide. The intensive use of chemical pesticides to control it has led to the selection of resistant populations. This study investigated the resistance of T. absoluta populations to pyrethroid and the organophosphate insecticides from ten regions of Iran. The resistance ratios at LC 50 for chlorpyrifos and diazinon varied among populations from 4.3 to 12 and from 1.4 to 9.0, respectively. The resistance ratios of the pyrethroids cypermethrin, deltamethrin and permethrin varied from 1.3 to 3.7, 2.7 to 13 and 1.2 to 4.3, respectively. Inclusion of synergists in toxicological bioassays and the variation observed in the activity of esterases, glutathione S-transferase and cytochrome P450-dependent monooxygenase suggest the existence of metabolically based resistance. Esterase and P450 biochemical assays were positively correlated with deltamethrin, and cypermethrin tolerance and diazinon tolerance correlated with esterase activity. The genes encoding the organophosphate and pyrethroid target sites acetylcholinesterase (ace1) and sodium channel (kdr) were partly sequenced. The genotyping revealed mutations in high frequencies in all populations leading to an A201S substitution in ace1 and three substitutions in the sodium channel gene L1014F, M918T, T929I. In summary, our results indicate the presence of organophosphate and pyrethroid resistance in Iranian T. absoluta populations with involvement of both detoxification enzymes and target site alterations. Most likely the populations of T. absoluta imported to Iran were resistant upon arrival. K E Y W O R D Sacetylcholinesterase, insecticide resistance, knock-down resistance, metabolic resistance, target site mutation, Tuta absoluta

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran

Zibaee

Mahmood

Esmaeily

et al. 2017

J Applied Entomology

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“…This insect invaded Europe in 2006 and spread to northern Africa in 2007 (Urbaneja et al, 2013), where it caused extensive economic losses to growers; multiple efforts have since been made to control this pest (Desneux et al, 2011). To aggravate this situation, resistance to insecticides has been reported for T. absoluta , making the development of alternative means of controlling this pest even more urgent (Urbaneja et al, 2013; Campos et al, 2014; Silva et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-A and Arginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet for T. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of dsRNA by leaflets and the second was based on “in planta-induced transient gene silencing” (PITGS), a well-established method for silencing plant genes, used here for the first time to deliver in planta-transcribed dsRNA to target insect genes. Tuta absoluta larvae that fed on leaves containing dsRNA of the target genes showed an ∼60% reduction in target gene transcript accumulation, an increase in larval mortality and less leaf damage. We then generated transgenic ‘Micro-Tom’ tomato plants that expressed hairpin sequences for both genes and observed a reduction in foliar damage by T. absoluta in these plants. Our results demonstrate the feasibility of RNAi as an alternative method for controlling this critical tomato pest.

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“…This may be even more important when considering a crop system such as tomatoes, where pest insect control interventions are intensively carried out with synthetic insecticide applications. However, in some cases, the desired outcome was not achieved due to control failures and pest resistance to insecticides (Campos, Silva, Silva, Silva, & Siqueira, ; Campos et al., ; Gontijo et al., ; Roditakis et al., ; Silva, Assis, Ribeiro, & Siqueira, ; Silva et al., , ; Siqueira, Guedes, & Picanço, ). Therefore, the management practices adopted aim at conserving and increasing the role natural control agents play as agroecosystem components, being able to help to reduce losses due to pest attack.…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation in natural mortality factors of Tuta absoluta (Lepidoptera: Gelechiidae) in open‐field tomato cultivation

Bacci

Silva

Martins

et al. 2018

J Applied Entomology

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The seasonal variation in natural mortality of phytophagous insects is determined by the relative importance of biotic and abiotic factors in agroecosystems. Knowledge regarding these factors throughout the year represents a key concern for IPM programmes. Seasonal population fluctuations of tomato pinworm, Tuta absoluta, led to an investigation of its natural mortality factors during the rainy season when the population level is low and during the dry season when population peaks occur. The aim of this study was to verify the seasonal variation in T. absoluta mortality factors in tomato crops. Immature stages of T. absoluta were obtained from laboratory‐rearing in the laboratory. These were taken to the field and monitored over two years. The mortality causes for each stage of insect development from egg to adult were assessed daily. Multiple biotic and abiotic mortality factors affected the immature T. absoluta stages such as rainfall, physiological disturbances, diseases, parasitoids and predators. The key T. absoluta mortality factor during summer–spring was predation. In addition, larvae predation correlated positively with temperature, wind velocity, photoperiod and rainfall. Nevertheless, during winter–fall, the key mortality factor was parasitism. Therefore, the critical stage for mortality was 3rd‐ and 4th‐instar larvae, being more vulnerable to natural control factors. Finally, the results showed the importance of vertical and horizontal action on natural mortality factors.

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Status of pyrethroid resistance and mechanisms in Brazilian populations of Tuta absoluta

Cited by 50 publications

References 39 publications

Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran

Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Seasonal variation in natural mortality factors of Tuta absoluta (Lepidoptera: Gelechiidae) in open‐field tomato cultivation

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