Status of the application of exogenous enzyme technology for the development of natural plant resources

Yuan, Bin; Zhou, Shuhua; Liu, Changwei; Zhang, Sheng; Li, Jiayin; Liu, Ailing

doi:10.1007/s00449-020-02463-w

Cited by 8 publications

(3 citation statements)

References 130 publications

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“…Camellia originated in Southeast Asia, and 80% of the species of this genus come from China. Theaceae species have high ornamental value and high economic value, such as medicine, food and drink ( Yuan et al, 2021 ). Tea is water soaked with Camellia leaves, known as “oriental magic water”, which is one of three most famous drinks in the world ( Balentine et al, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Codon Bias in the Chloroplast Genomes of Theaceae Species

et al. 2022

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Theaceae species are dicotyledonous angiosperms with extremely high ornamental and economic value. The chloroplast genome is traditionally used to study species evolution, expression of chloroplast genes and chloroplast transformation. Codon usage bias (CUB) analysis is beneficial for investigations of evolutionary relationships and can be used to improve gene expression efficiency in genetic transformation research. However, there are relatively few systematic studies of the CUB in the chloroplast genomes of Theaceae species. In this study, CUB and nucleotide compositions parameters were determined by the scripts written in the Perl language, CodonW 1.4.2, CU.Win2000, RStudio and SPSS 23.0. The chloroplast genome data of 40 Theaceae species were obtained to analyse the codon usage (CU) characteristics of the coding regions and the influence of the source of variation on CUB. To explore the relationship between the CUB and gene expression levels in these 40 Theaceae plastomes, the synonymous codon usage order (SCUO) and measure independent of length and composition (MILC) values were determined. Finally, phylogenetic analysis revealed the genetic evolutionary relationships among these Theaceae species. Our results showed that based on the chloroplast genomes of these 40 Theaceae species, the CUB was for codons containing A/T bases and those that ended with A/T bases. Moreover, there was great commonality in the CUB of the Theaceae species according to comparative analysis of relative synonymous codon usage (RSCU) and relative frequency of synonymous codon (RFSC): these species had 29 identical codons with bias (RSCU > 1), and there were 19 identical high-frequency codons. The CUB of Theaceae species is mainly affected by natural selection. The SCUO value of the 40 Theaceae species was 0.23 or 0.24, and the chloroplast gene expression level was moderate, according to MILC values. Additionally, we observed a positive correlation between the SCUO and MILC values, which indicated that CUB might affect gene expression. Furthermore, the phylogenetic analysis showed that the evolutionary relationships in these 40 Theaceae species were relatively conserved. A systematic study on the CUB and expression of Theaceae species provides further evidence for their evolution and phylogeny.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Codon Bias in the Chloroplast Genomes of Theaceae Species

et al. 2022

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“…Нині ароматизатори становлять більше чверті світового ринку харчових добавок. Попит на натуральні ароматизатори постійно зростає, тоді як синтетичні все менше затребувані [3]. Людина оцінює аромат суб'єктивно, більшою мірою ірраціонально, але обізнаність щодо походження та природи ароматизатора є важливим фактором при виборі продукту харчування.…”

Section: вступunclassified

Application of Aroma Precursors in Food Plant Raw Materials: Biotechnological Aspect

Dubova

Bezusov²,

Biloshytska

et al. 2022

Innov Biosyst Bioeng

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The article is devoted to the analysis of the main factors accompanying the use of aroma precursors, in particular, of a lipid nature, in food raw materials. The prerequisites for the impact on the precursors of aroma with the help of plant enzymes are given. The purpose of the article is to analyze the biotechnological aspect, which is based on enzymatic reactions with aroma precursors and enzymes of plant origin. Features of the mechanism of action of lipid precursors are highlighted, their diversity causing various characteristic reactions is analyzed, and possible end products of reactions with certain odors are noted. The attention is paid to the issue of the status of the naturalness of flavor precursors in food products, which varies in different countries. A scheme of factors influencing the formation of aroma from lipid precursors has been developed. The influence of pigments of carotenoid nature on the aroma is considered, namely: examples of instantaneous change of watermelon aroma to pumpkin one due to isomerization of carotenoids are given. The main factors of enzymatic formation of aroma from precursors of polyunsaturated fatty acids for their effective use by creating micromicelles are summarized. A way to overcome the barrier of interaction between lipid precursors of a hydrophobic nature and hydrophilic enzymes has been substantiated. It is proposed to accelerate enzymatic reactions under in vitro conditions and use the vacuum effect to overcome the barrier between enzymes and precursors. To explain the effect of vacuum in a system with enzymes, ideas about disjoining pressure and the reasonable expediency of its use are considered. A schematic process flow diagram for the restoration of aroma lost during the technological processing of raw materials is given; it demonstrates the factors for ensuring interfacial activation conditions for enzymes and aroma precursors.

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“…Biorefinery platform via enzymes for generation of industrially available commodities from these lignocellulosic wastes are consistent with the new paradigm of a circular, sustainable and low-carbon bioeconomy [ 2 ]. Lignocellulolytic enzymes take up more than 20% of sales of commercial enzyme around the worldwide [ 3 ]. Laccases (EC.1.10.3.2, benzenediol: O 2 oxidoreductase) widely distribute in fungi, bacteria, plants and insects, and have been overproduced by genetically modified strains [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The feasibility of sodium hydroxide pretreatment of rice straw for solid substrate preparation to enhance laccase production by solid state fermentation

Wang

Gao

et al. 2023

BMC Biotechnol

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Background Currently, broad industrial application of laccases is commonly restricted by the high-cost related production. Solid state fermentation (SSF) using agricultural waste is an attractively economic strategy for laccase production, yet its efficiency is low. Pretreatment of cellulosic substrate might be a vital breakpoint to solve the problem in solid state fermentation (SSF). In this study, sodium hydroxide pretreatment was involved to prepare solid substrates from rice straw. Fermentability of solid substrates in terms of carbon resource supply, accessibility and water retention value, and their influence on performance of SSF were analyzed. Results The results showed that sodium hydroxide pretreatment provided desirable solid substrates with higher enzymatic digestibility and optimal water retention value, which further facilitated the homogeneity of mycelium growth, laccase distribution and nutrition utilization during SSF. The pretreated rice straw (1 h) with diameter less than 0.085 cm gave the maximum laccase production of 2912.34 U/g, which was 7.72 times higher than the control. Conclusion Hence, we proposed that enough balance between nutrition accessibility and structure support was a must for rational design and preparation of solid substrate. Additionally, sodium hydroxide pretreatment of lignocellulosic waste might be an ideal step to enhance the efficiency and lower the production cost in SSF.

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Status of the application of exogenous enzyme technology for the development of natural plant resources

Cited by 8 publications

References 130 publications

Comparative Analysis of Codon Bias in the Chloroplast Genomes of Theaceae Species

Comparative Analysis of Codon Bias in the Chloroplast Genomes of Theaceae Species

Application of Aroma Precursors in Food Plant Raw Materials: Biotechnological Aspect

The feasibility of sodium hydroxide pretreatment of rice straw for solid substrate preparation to enhance laccase production by solid state fermentation

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