Steady-state and time-resolved emission studies of Thioflavin-T

Naik, L. R.; Naik, A. B.; Pal, Haridas

doi:10.1016/j.jphotochem.2009.03.016

Cited by 30 publications

(32 citation statements)

References 19 publications

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“…In the presence of PSS, the absorption maximum is shifted to 418 nm. Similar shift was observed for ThT spectra in less polar environment (DMSO) (Naik et al 2009). PSS contains hydrophobic benzene group which can be responsible for maximum position shift.…”

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confidence: 81%

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Polyanion-induced circular dichroism of Thioflavin T

Fedunova¹,

Huba²,

Bágeľová³

et al. 2013

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Abstract. Thioflavin T (ThT) is amyloid specific fluorescence dye possessing the properties of molecular rotor. We have shown that Thioflavin T forms complexes with non-peptide polyanions heparin, polyadenylate and polystyrene sulphonate by means of absorption spectroscopy. In the presence of chiral polyanions -heparin and polyadenylate -induced optical activity of ThT occurs whereas interaction with achiral polystyrene sulphonate (PSS) does not lead to production of induced circular dichroism signal. The positively charged ThT forms centre for ordered binding of chiral polyanion. Similarly, complexation of structurally different chromophore 9-aminoacridine with polyanions has led to induction of optical activity only in the presence of chiral ones. We suggest that, primarily, the optical activity of environment plays important role in inducing optical activity of achiral compounds.

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confidence: 81%

“…2A illustrates the absorption spectra of ThT at the presence of various polyanions. The absorption band of free ThT at 412 nm corresponds to ThT monomer (Naik et al 2009). In the complex with polyadenylate, narrower absorption peak occurs with maximum at 412 nm and shoulder at 455 nm.…”

mentioning

confidence: 99%

Polyanion-induced circular dichroism of Thioflavin T

Fedunova¹,

Huba²,

Bágeľová³

et al. 2013

gpb

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“…[13][14][15][16][17][18][19] Steady-state fluorescence experiments reveal strong a viscosity dependence of the fluorescence quantum yield. [20][21][22] Quantum chemical calculations suggest that the S 1 state of the molecule is an intramolecular charge-transfer (ICT) state with the mutually twisted benzothiazole moiety and the dimethylaniline group. [23] Time-resolved fluorescence experiments with femtosecond time resolution reveal the ultrafast rotor behavior, which is strongly viscosity dependent.…”

Section: Introductionmentioning

confidence: 99%

Ultrafast Twisting Dynamics of Thioflavin‐T: Spectroscopy of the Twisted Intramolecular Charge‐Transfer State

Ghosh¹,

Palit²

2014

ChemPhysChem

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Understanding the excited-state properties of thioflavin-T (ThT) has been of immense importance, because of its efficient amyloid-sensing ability related to neurodegenerative disorders. The excited-state dynamics of ThT is studied by using sub-pico- and nanosecond time-resolved transient absorption techniques as well as density functional theory (DFT)/time-dependent DFT calculations. Barrierless twisting around the central C-C bond between two aromatic moieties is the dominant process that contributes to the ultrafast dynamics of the S1 state. The spectroscopic properties of the intramolecular charge-transfer state are characterized for the first time. The energetics of the S0 and S1 states has also been correlated with the experimentally observed spectroscopic parameters and structural dynamics. A longer-lived transient state populated with a very low yield has been characterized as the triplet state.

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“…In the excited state it undergoes a twisted intramolecular charge-transfer (TICT) state and this charge transfer is facilitated in low viscosity solvents. [4,5] These TICT state formations take place through ultrafast torsional motion along the CÀC bond that connects the benzothiazole ring and dimethylaniline moieties. Several researchers extensively studied the photophysical properties of ThT in different media.…”

Section: Introductionmentioning

confidence: 99%

Torsional Dynamics of Thioflavin T in Room‐Temperature Ionic Liquids: An Effect of Heterogeneity of the Medium

2013

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The ultrafast excited-state dynamics of a fibril binding dye, thioflavin T (ThT), has been studied in two room-temperature ionic liquids (RTILs): 1-Butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide and methyltrioctylammonium bis(trifluoromethylsulfonyl)imide. Previously, in most studies, it was observed that the excited-state dynamics of the ThT dye were dependent on the viscosity of the medium. In our study, by using RTILs, we have demonstrated that the excited-state dynamics of ThT are not only viscosity dependent, but also dependent on the heterogeneous nature of the medium. The effect of structural heterogeneity present in neat RTILs on the excited-state dynamics of ThT was observed. For both RTILs, excitation wavelength dependency on the emission properties of ThT was observed.

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Steady-state and time-resolved emission studies of Thioflavin-T

Cited by 30 publications

References 19 publications

Polyanion-induced circular dichroism of Thioflavin T

Polyanion-induced circular dichroism of Thioflavin T

Ultrafast Twisting Dynamics of Thioflavin‐T: Spectroscopy of the Twisted Intramolecular Charge‐Transfer State

Torsional Dynamics of Thioflavin T in Room‐Temperature Ionic Liquids: An Effect of Heterogeneity of the Medium

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