Steady-state fluorescence study on release of camptothecin from agar hydrogel

Liu, J.; Chen, L.; Li, L.; Hu, Xiao; Cai, Yiyu

doi:10.1016/j.ijpharm.2004.08.010

Cited by 28 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The calibration curve was obtained with at a CPT concentration varying from 3 ng/ml to 100 ng/ml. After 10x dilution using PBS, the fluorescence intensity of the test samples (three replicates for each sample) was collected and the CPT concentration was determined using the calibration curve [34, 35]. …”

Section: Methodsmentioning

confidence: 99%

Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells

Gurski¹,

Jha²,

Zhang³

et al. 2009

Biomaterials

239

183

View full text Add to dashboard Cite

The current investigation aimed to develop a biomimetic, three-dimensional (3D) culture system for poorly adherent bone metastatic prostate cancer cells (C4-2B) for use as an in vitro platform for anti-cancer drug screening. To this end, hyaluronic acid (HA) derivatives carrying complementary aldehyde (HAALD) and hydrazide (HAADH) groups were synthesized and characterized. In situ encapsulation of C4-2B cells was achieved by simple mixing of HAALD and HAADH in the presence of the cells. Unlike two-dimensional (2D) monolayer culture in which cells adopt an atypical spread morphology, cells residing in the HA matrix formed distinct clustered structures which grew and merged, reminiscent of real tumors. Anti-cancer drugs added to the media surrounding the cell/gel construct diffused into the gel and killed the embedded cells. The HA hydrogel system was used successfully to test the efficacy of anti-cancer drugs including camptothecin, docetaxel, and rapamycin, alone and in combination, including specificity, dose and time responses. Responses of cells to anti-neoplastics differed between the 3D HA hydrogel and 2D monolayer systems. We suggest that the data obtained from 3D HA systems is superior to that from conventional 2D monolayers as the 3D system better reflects the bone metastatic microenvironment of the cancer cells.

show abstract

Section: Methodsmentioning

confidence: 99%

Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells

Gurski¹,

Jha²,

Zhang³

et al. 2009

Biomaterials

239

183

View full text Add to dashboard Cite

show abstract

“…The presence of a conjugated pyridine moiety in CPT allows its concentration to be accurately determined by spectrofluorometer [18, 22, 23]. No CPT precipitation was expected under the experimental conditions employed.…”

Section: Resultsmentioning

confidence: 99%

“…The dry powder was weighted accurately before being thoroughly dissolved in 1 mL of THF/DMSO (v/v, 4/1). The CPT concentration in the organic solvent was determined by a FluoroMax-3 spectrofluorometer at an excitation wavelength of 374 nm and an emission wavelength of 432 nm [18, 22, 23]. The encapsulation efficiency (as a % of total) was calculated by dividing the amount of CPT loaded into the particles with the amount of CPT initially added during the particle preparation.…”

Section: Methodsmentioning

confidence: 99%

Amphiphilic Block Co-polyesters Bearing Pendant Cyclic Ketal Groups as Nanocarriers for Controlled Release of Camptothecin

Wang

Gurski

Zhong

et al. 2011

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

Amphiphilic block copolymers consisting of hydrophilic poly(ethylene glycol) and hydrophobic polyester bearing pendent cyclic ketals were synthesized by ring-opening copolymerization of ε-caprolactone (CL) and 1,4,8-trioxaspiro-[4,6]-9-undecanone (TSU) using α-hydroxyl, ω-methoxy, polyethylene glycol as the initiator and stannous octoate as the catalyst. Compositional analyses indicate that TSU was randomly distributed in the hydrophobic blocks. When the TSU content in the copolymers increased, the polymer crystallinity decreased progressively and the glass transition temperature increased accordingly. Hydrophobic, anticancer drug, camptothecin (CPT), was successfully encapsulated in the block copolymer nanoparticles. The CPT encapsulation efficiency and release kinetics were strongly dependent on the copolymer composition and crystallinity. CPT release from nanoparticles constructed from copolymers containing 0, 39 and 100 mol% TSU in the hydrophobic block followed the same trend, with an initial burst of ~40% within one day followed by a moderate and slow release lasting up to 7 days. At a TSU content of 14 mol%, CPT was released in a continuous and controlled fashion with a reduced initial burst and a 73% cumulative release by day 7. In vitro cytoxicity assay showed that the blank nanoparticles were not toxic to the cultured bone metastatic prostate cancer cells (C4-2B). Compared to the free drug, the encapsulated CPT was more effective in inducing apoptotic responses in C4-2B cells. Modulating the physical characteristics of the amphiphilic copolymers via copolymerization offers a facile method for controlling the bioavailability of anticancer drugs ultimately increasing effectiveness and minimizing toxicity.

show abstract

“…DB-67 possesses a characteristic intense fluorescence, allowing accurate detection of concentration in aqueous solution even though the drug is only sparingly soluble [38,39]. The fluorescence excitation/emission spectrum of a 10 lg ml À1 DB-67 solution prepared in 1 N NaOH is shown in Fig.…”

Section: In Vitro Drug Releasementioning

confidence: 99%

LDI–glycerol polyurethane implants exhibit controlled release of DB-67 and anti-tumor activity in vitro against malignant gliomas

et al. 2008

View full text Add to dashboard Cite

Steady-state fluorescence study on release of camptothecin from agar hydrogel

Cited by 28 publications

References 29 publications

Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells

Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells

Amphiphilic Block Co-polyesters Bearing Pendant Cyclic Ketal Groups as Nanocarriers for Controlled Release of Camptothecin

LDI–glycerol polyurethane implants exhibit controlled release of DB-67 and anti-tumor activity in vitro against malignant gliomas

Contact Info

Product

Resources

About