Steady-State Pharmacokinetic Properties of Tamsulosin in Healthy Male Volunteers

Seong, Sook Jin; Lee, Hae Won; Lee, Joomi; Lim, Myung-Seop; Kim, Eun Hee; Park, Sung Min; Gwon, Mi Ri; Yoon, Young Cheol

doi:10.12793/jkscpt.2013.21.2.130

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Silodosin (Recordati, Milan, Italy), tamsulosin (Cayman, Ann Arbor, MI, USA), and naftopidil (Cayman) were procured and used. Silodosin and tamsulosin were dissolved in ethanol and naftopidil in methanol (Merck, Kenilworth, NJ, USA) and stored as stock solutions: silodosin (1 mM), tamsulosin (400 nM), and naftopidil (200 µM) [ 15 16 17 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of α1D-adrenoceptor blocker for the reduction of ureteral contractions

et al. 2023

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Purpose Urolithiasis is a common urinary tract disease with growing prevalence. Alpha1-adrenoceptors (α1-ARs) are abundant in ureteral smooth muscle, distributed with different α1-AR subtypes. α1D-AR is the most widely distributed in the ureter. However, the effect of α1D-AR blockade on ureteric contraction remains unknown. Materials and Methods We dissected smooth muscle tissues (3 mm×3 mm) from the rat bladder and human ureter, tied silk strips on both tissue ends, and measured contraction in an organ bath chamber. Contraction activity in ureteral smooth muscle cells (USMCs) was immunocytochemically examined using primary rat and human USMC cultures. Results Using the organ bath system, we determined the inhibitory effects of silodosin, tamsulosin, and naftopidil. Naftopidil significantly decreased contractility of rat bladder tissue; similar results were observed in human ureteral tissue. To confirm ex vivo experimental results in vitro , we examined the phosphorylation of myosin light chain (MLC), a marker of contractility, in a primary human USMC culture. The examined drugs decreased phospho-MLC levels in human USMCs; however, naftopidil profoundly increased MLC dephosphorylation. Conclusions We studied the effects of naftopidil, an α1D-AR inhibitor, on the ureter. Compared with alpha-blockers, naftopidil significantly relaxed ureteral smooth muscle. Therefore, naftopidil could be an effective therapy for patients with ureteral stones.

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Section: Methodsmentioning

confidence: 99%