Steady‐State Spectrofluorometry

Valeur, Bernard; Berberan‐Santos, Mário Nuno

doi:10.1002/9783527650002.ch9

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…However, these correction methods often require complicated experimental setups, significant time for data collection, and do not provide notable reduction in errors. Therefore, it has been suggested that front-face measurements (such as the one shown in Figure 1b) offer significant advantages over these other techniques; the front-face measurement geometry avoids the excitation and emission paths passing through the entire sample [33], which makes correcting for IFE simpler. It has recently been shown that it is extremely difficult to completely eliminate the IFE from any measurements, and the IFE can only be minimised by use of front-face measurements on thin-film samples [29].…”

Section: The Inner Filter Effect (Ife)mentioning

confidence: 99%

Analysing the Photo-Physical Properties of Liquid Crystals

Hobbs,

Mattsson,

Nagaraj

2024

Crystals

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Intrinsically fluorescent liquid crystals are highly sought after for a variety of applications. Most of the measurements of photo-physical properties of liquid crystals are made in dilute solutions, which is mainly due to the relative ease of both these measurements and the interpretation of data. The fluorescence spectra depend on a number of parameters including the concentration in liquid crystal solutions, the device geometry, and the mesophase in which the spectra have been measured. Working with neat, or concentrated, liquid crystal samples adds experimental complexities such as the inner filter effect (IFE), which affects the collection of data, interpretation of the results, and accuracy of the conclusions. In this paper, we present a systematic study of the photo-physical properties of both a model reference material, Nile red, and a nematic liquid crystal, 4-cyano-4′-pentylbiphenyl (5CB). The influence on the emission spectra of an increasing solute concentration is investigated and discussed. Moreover, a detailed investigation of the influence of the used device geometry, as well as the choice of appropriate data fitting methodologies, are presented.

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Section: The Inner Filter Effect (Ife)mentioning

confidence: 99%

Analysing the Photo-Physical Properties of Liquid Crystals

Hobbs,

Mattsson,

Nagaraj

2024

Crystals

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Affinity Protein-Based FRET Tools for Cellular Tracking of Chitosan Nanoparticles and Determination of the Polymer Degree of Acetylation

et al. 2014

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Chitosan (CS) is a family of linear polysaccharides with diverse applications in medicine, agriculture, and industry. Its bioactive properties are determined by parameters such as the degree of acetylation (DA), but current techniques to measure the DA are laborious and require large amounts of substrate and sophisticated equipment. It is also challenging to monitor the fate of chitosan-based nanoparticles (CS-NPs) in vitro because current tools cannot measure their enzymatic or chemical degradation. We have developed a method based on the Förster resonance energy transfer (FRET) that occurs between two independent fluorescent proteins fused to a CS-binding domain, who interact with CS polymers or CS-NPs. We used this approach to calibrate a simple and rapid analytical method that can determine the DA of CS substrates. We showed unequivocally that FRET occurs on the surface of CS-NPs and that the FRET signal is quenched by enzymatic degradation of the CS substrate. Finally, we provide in vitro proof-of-concept that these approaches can be used to label CS-NPs and colocalize them following their interactions with mammalian cells.

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Multimodal Integrated Sensor Platform for Rapid Biomarker Detection

Al-Rawhani

Mitra

Barrett

et al. 2020

IEEE Trans. Biomed. Eng.

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Precision metabolomics and quantification for costeffective, rapid diagnosis of disease are key goals in personalized medicine and point-of-care testing. Presently, patients are subjected to multiple test procedures requiring large laboratory equipment. Microelectronics has already made modern computing and communications possible by integration of complex functions within a single chip. As More than Moore technology increases in importance, integrated circuits for densely patterned sensor chips have grown in significance. Here, we present a versatile single CMOS chip forming a platform to address personalized needs through on-chip multimodal optical and electrochemical detection that will reduce the number of tests that patients must take. The chip integrates interleaved sensing subsystems for quadruplemode colorimetric, chemiluminescent, surface plasmon resonance and hydrogen ion measurements. These subsystems include a photodiode array and a single photon avalanche diode array, with some elements functionalized to introduce a surface plasmon resonance mode. The chip also includes an array of ion sensitive field effect transistors. The sensor arrays are distributed uniformly over an active area on the chip surface in a scalable and modular design. Bio-functionalization of the physical sensors yields a highly selective simultaneous multiple-assay platform in a disposable format. We demonstrate its versatile capabilities through quantified bioassays performed on-chip for glucose, cholesterol, urea and urate, each within their naturally occurring physiological range.

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Steady‐State Spectrofluorometry

Cited by 3 publications

References 11 publications

Analysing the Photo-Physical Properties of Liquid Crystals

Analysing the Photo-Physical Properties of Liquid Crystals

Affinity Protein-Based FRET Tools for Cellular Tracking of Chitosan Nanoparticles and Determination of the Polymer Degree of Acetylation

Multimodal Integrated Sensor Platform for Rapid Biomarker Detection

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