Stearoyl-CoA desaturase (<i>scd1a</i>) is epigenetically regulated by broodstock nutrition in gilthead sea bream (<i>Sparus aurata</i>)

Perera, Erick; Türkmen, Serhat; Simó‐Mirabet, Paula; Zamorano, M. J.; Xu, Hanlin; Naya-Català, Fernando; Izquierdo, Marisol; Pérez‐Sánchez, Jaume

doi:10.1080/15592294.2019.1699982

Cited by 31 publications

(14 citation statements)

References 84 publications

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“…Therefore, the up-regulation of scd1b and elovl6 enhances the biosynthesis of MUFA, which in turn increases the unsaturation index of FA membrane phospholipids. This is reinforced by the recent observation of Perera et al (2019) that scd1a is epigenetically regulated in gilthead sea bream by broodstock nutrition with alpha-linolenic acid (ALA, 18:3n-3) enriched diets.…”

Section: Discussionmentioning

confidence: 95%

“…When revisiting the gene expression profile of gilthead sea bream fed isolipidic diets with a maximal replacement of FO by vegetable oils or semisynthetic diets formulated to be deficient in n-3 LC-PUFA, the most responsive hepatic enzymes of lipid metabolism are scd1 and elovl6, and secondly fads2 (Benedito-Palos et al, 2016). This will contribute to mitigate the signs of deficiencies in n-3 LC-PUFA, though it is very important to limit hepatic lipogenesis to avoid the lipotoxic effects of excessive fat accumulation (Perera et al, 2019). Indeed, scd1 is the rate limiting enzyme in the synthesis of MUFA, especially oleic acid (OA, 18:1n-9) and palmitoleic acid (16:1n-7) from stearoyl-CoA and palmitoyl-CoA, respectively.…”

Section: Discussionmentioning

confidence: 99%

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The Use of Defatted Tenebrio molitor Larvae Meal as a Main Protein Source Is Supported in European Sea Bass (Dicentrarchus labrax) by Data on Growth Performance, Lipid Metabolism, and Flesh Quality

et al. 2021

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ObjectiveThis study aims to determine the maximal inclusion level of defatted (d-) Tenebrio molitor larvae meal (TM) able to replace dietary fishmeal (FM) without compromising growth performance, general metabolism, and flesh quality traits in European sea bass, and to evaluate the major underlying physiological mechanisms.Materials and MethodsFish (55 ± 2 g) were fed with diets containing increasing levels of dTM: 0, 40, 80 and 100% (CTRL, TM40, TM80, and TM100, respectively) to replace FM. After 10 weeks of feeding, the growth performance, nutrient and energy balance, intestinal integrity, plasma metabolites and the expression of genes related to growth and nutrient metabolism, in liver and muscle were determined. The fatty acids (FA) profile, textural properties and color were also evaluated in muscle.ResultsProtein and lipids digestibility remained unaltered up to 80% dTM inclusion. Growth performance parameters were similar among dietary treatments. The dTM inclusion increased the hepatosomatic index in fish fed TM100. Muscle eicosapentaenoic acid, docosahexaenoic acid and n-3 long-chain polyunsaturated FA levels were maintained up to 80% dTM inclusion, but total cholesterol and non-esterified FA increased with dietary dTM inclusion. In liver, the expression of elongation of very long-chain FA protein 6 (elovl6) and FA desaturase 2 (fads2) did not change in fish fed TM40 and TM80, but elovl6 decreased whilst fads2 increased in fish fed TM100 when compared to those fed CTRL. The expression of cholesterol 7 alpha-monooxygenase (cyp7a1) decreased with dietary dTM inclusion. In muscle, the expression of myoblast determination protein-2 (myod2) decreased in fish fed TM80 and TM100.ConclusionIt is feasible to substitute dietary FM by dTM up to 80% in European sea bass without detrimental effects on nutrient digestibility, growth performance and associated genetic pathways, whilst assuring fillet nutritional value for human consumption.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

The Use of Defatted Tenebrio molitor Larvae Meal as a Main Protein Source Is Supported in European Sea Bass (Dicentrarchus labrax) by Data on Growth Performance, Lipid Metabolism, and Flesh Quality

et al. 2021

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“…However, in fish, these relationships remain in an infancy state, reinforcing the value of this study where, for the first time in a typical marine fish, a possible link between enzymes of lipid metabolism and gut microbiota was evidenced. Here, this can be exemplified by the lipogenic scd1a and scd1b enzymes, which becomes especially interesting in the case of scd1a given that a recent study revealed reliable epigenetic mechanisms (changes in DNA methylation rates of the scd1a promoter) by which the nutrition of parents can shape the s cd1a gene expression in the gilthead sea bream offspring (Perera et al, 2020 ). The extent to which this can be favored by changes in the gut microbiota remains unknown, but interestingly, the up-regulation of scd1 genes in the fish fed with the non-PAP diet was associated with OTUs assigned to Serratia nematodiphila (NoPAP, 1.02%) and Brevinema andersonii (NoPAP 0.1%; CTRL and PAP <0.01%), giving these taxa a role as intestinal SCFA producers in humans (Parada Venegas et al, 2019 ) and enhancers of fatty acid metabolism in insects (Zhou et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (Sparus aurata) Juveniles: Insights in Fish Feeds for Increased Circularity and Resource Utilization

et al. 2021

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New types of fish feed based on processed animal proteins (PAPs), insect meal, yeast, and microbial biomasses have been used with success in gilthead sea bream. However, some drawback effects on feed conversion and inflammatory systemic markers were reported in different degrees with PAP- and non-PAP-based feed formulations. Here, we focused on the effects of control and two experimental diets on gut mucosal-adherent microbiota, and how it correlated with host transcriptomics at the local (intestine) and systemic (liver and head kidney) levels. The use of tissue-specific PCR-arrays of 93 genes in total rendered 13, 12, and 9 differentially expressed (DE) genes in the intestine, liver, and head kidney, respectively. Illumina sequencing of gut microbiota yielded a mean of 125,350 reads per sample, assigned to 1,281 operational taxonomic unit (OTUs). Bacterial richness and alpha diversity were lower in fish fed with the PAP diet, and discriminant analysis displayed 135 OTUs driving the separation between groups with 43 taxa correlating with 27 DE genes. The highest expression of intestinal pcna and alpi was achieved in PAP fish with intermediate values in non-PAP, being the pro-inflammatory action of alpi associated with the presence of Psychrobacter piscatorii. The intestinal muc13 gene was down-regulated in non-PAP fish, with this gene being negatively correlated with anaerobic (Chloroflexi and Anoxybacillus) and metal-reducing (Pelosinus and Psychrosinus) bacteria. Other inflammatory markers (igm, il8, tnfα) were up-regulated in PAP fish, positively correlating the intestinal igm gene with the inflammasome activator Escherichia/Shigella, whereas the systemic expression of il8 and tnfα was negatively correlated with the Bacilli class in PAP fish and positively correlated with Paracoccus yeei in non-PAP fish. Overall changes in the expression pattern of il10, galectins (lgals1, lgals8), and toll-like receptors (tlr2, tlr5, tlr9) reinforced the anti-inflammatory profile of fish fed with the non-PAP diet, with these gene markers being associated with a wide range of OTUs. A gut microbiota-liver axis was also established, linking the microbial generation of short chain fatty acids with the fueling of scd1- and elovl6-mediated lipogenesis. In summary, by correlating the microbiome with host gene expression, we offer new insights in the evaluation of fish diets promoting gut and metabolism homeostasis, and ultimately, the health of farmed fish.

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“…In livestock fish, special emphasis on epigenetic regulation has been focused on the nutritional programming of lipid metabolism 14 – 16 , and differential promoter DNA methylation supports adaptive offspring changes in the gene expression of the delta 9 fatty acid desaturase ( scd1a ) in gilthead sea bream ( Sparus aurata ) fed plant-based diets 17 . Also, as reviewed by Jonsson and Jonsson 18 , there is now evidence that the offspring performance of fish is affected by the temperature during embryo development or by the temperature experienced by parents prior to egg fertilization 19 , 20 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream

Naya-Català

Simó‐Mirabet

Calduch-Giner

et al. 2021

Sci Rep

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A customized PCR-array was used for the simultaneous gene expression of the Gh/Igf system and related markers of muscle growth, and lipid and energy metabolism during early life stages of gilthead sea bream (60–127 days posthatching). Also, transcriptional reprogramming by mild hypoxia was assessed in fingerling fish with different history trajectories on O2 availability during the same time window. In normoxic fish, the expression of almost all the genes in the array varied over time with a prompted liver and muscle tissue-specific differentiation, which also revealed temporal changes in the relative expression of markers of the full gilthead sea bream repertoire of Gh receptors, Igfs and Igf-binding proteins. Results supported a different contribution through development of ghr and igf subtypes on the type of action of GH via systemic or direct effects at the local tissue level. This was extensive to Igfbp1/2/4 and Igfbp3/5/6 clades that clearly evolved through development as hepatic and muscle Igfbp subtypes, respectively. This trade-off is however very plastic to cope changes in the environment, and ghr1 and igfbp1/3/4/5 emerged as hypoxic imprinting genes during critical early developmental windows leading to recognize individuals with different history trajectories of oxygen availability and metabolic capabilities later in life.

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Stearoyl-CoA desaturase (scd1a) is epigenetically regulated by broodstock nutrition in gilthead sea bream (Sparus aurata)

Cited by 31 publications

References 84 publications

The Use of Defatted Tenebrio molitor Larvae Meal as a Main Protein Source Is Supported in European Sea Bass (Dicentrarchus labrax) by Data on Growth Performance, Lipid Metabolism, and Flesh Quality

The Use of Defatted Tenebrio molitor Larvae Meal as a Main Protein Source Is Supported in European Sea Bass (Dicentrarchus labrax) by Data on Growth Performance, Lipid Metabolism, and Flesh Quality

Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (Sparus aurata) Juveniles: Insights in Fish Feeds for Increased Circularity and Resource Utilization

Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream

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