2015
DOI: 10.1016/j.celrep.2015.02.007
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STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons

Abstract: In the axons of cultured hippocampal neurons, actin forms various structures, including bundles, patches (involved in the preservation of neuronal polarity), and a recently reported periodic ring-like structure. Nevertheless, the overlaying organization of actin in neurons and in the axon initial segment (AIS) is still unclear, due mainly to a lack of adequate imaging methods. By harnessing live-cell stimulated emission depletion (STED) nanoscopy and the fluorescent probe SiR-Actin, we show that the periodic s… Show more

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Cited by 285 publications
(411 citation statements)
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“…Recent studies by subdiffraction fluorescence microscopy have revealed a periodic distribution of ␤II and ␤IV spectrins and F-actin in axons, some dendrites (Xu et al, 2013;Zhong et al, 2014;D'Este et al, 2015;Leterrier et al, 2015;D'Este et al, 2016;He et al, 2016;Sidenstein et al, 2016), and even in dendritic spines (Bär et al, 2016). Periodicity of ␤III spectrin was noted only in occasional small regions of dendrites (Zhong et al, 2014).…”
Section: Discussionmentioning
confidence: 99%
“…Recent studies by subdiffraction fluorescence microscopy have revealed a periodic distribution of ␤II and ␤IV spectrins and F-actin in axons, some dendrites (Xu et al, 2013;Zhong et al, 2014;D'Este et al, 2015;Leterrier et al, 2015;D'Este et al, 2016;He et al, 2016;Sidenstein et al, 2016), and even in dendritic spines (Bär et al, 2016). Periodicity of ␤III spectrin was noted only in occasional small regions of dendrites (Zhong et al, 2014).…”
Section: Discussionmentioning
confidence: 99%
“…NR appear to present a similar architecture (33,34). The periodic cytoskeleton lattice was also observed in distal axons, where actin rings alternate with ␤II-spectrin (32)(33)(34). In that case, actin rings were proposed to be connected by typical head-to-tail ␤II-spectrin/␣-spectrin tetramers.…”
Section: Discussionmentioning
confidence: 97%
“…The nanoscale architecture of AIS was recently resolved, showing that the actin/␤IV-spectrin/AnkG submembranous cytoskeleton is organized as a periodic lattice of proteins that alternate between actin rings and ␤IV-spectrin⅐AnkG com- plexes (32)(33)(34)(35). NR appear to present a similar architecture (33,34).…”
Section: Discussionmentioning
confidence: 99%
“…As contiguous SRCPs, a Spectrin tetramer is about 180-190 nm long, which is confirmed by super-resolution microscopy. 15,16 Moreover, the physiological resting length of spectrin can undergo a 3-fold extension, which can be explained by a compact supercoil model. 17 Excluding the linker regions between SRs, a giant isoform of Nesprin-1 possesses 74 SRs and MSP300 at least 78 SRs 18 (our unpublished data).…”
Section: Nesprins Are Required For Tensional Homeostasis and Force Trmentioning
confidence: 99%
“…For example factors controlling MT polarity around the myonucleus and their dynamic behavior are yet to be characterized. Furthermore, the architectural features, such as oligomerization patterns and in vivo elastic behavior of tandem SRs in large number should be further characterized by approaches such as nanoscale microscopy 15,16 and 2-dimensional infrared spectroscopy (2DIR). We believe and hope that the present data would encourage scientists of bioengineering background to further address the bioarchitecture of myonuclei in more quantitative approaches.…”
Section: "Faster Higher Stronger:" Breaking Bottlenecks In Muscle Nmentioning
confidence: 99%