Steinernema kandii n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from northern Benin

Abstract

SummaryTwo nematode isolates from the genusSteinernemawere collected in northern Benin. Morphological, morphometric, molecular and cross-hybridisation studies placed these nematodes into a new species,Steinernema kandiin. sp., within thebicornutum-group. Phylogenetic analyses based on both ITS and D2-D3 regions of 28S rDNA revealed thatS. kandiin. sp. is different from all knownSteinernemaspecies and sister toS. abbasi(97.3-97.6% ITS nucleotide similarity) andS. bifurcatum(98.3-98.4% D2-D3 similarity).Steinernema kandiin. sp. can be separated from other members of thebicornutum-group by the greater infective juvenile (IJ) max. body diam. of 35 (27-48)μm (type isolate). It differs fromS. abbasiby the greater IJ body length 707 (632-833)μm (type isolate), EP distance 55 (52-60)μm (type isolate), spicule length 67 (57-75)μm (type isolate) and the occurrence of one pair of genital papillae at the cloacal aperture.

Molecular characterization of Insect Parasitic nematodes, and assonated Symbiotic bacteria as Ecotoxic effect of Invasive insect larvae

“…al., 2018;Srivastava et al, 2022) is signi cantly closer to S. feltiae and tree sequences of S. feltiae populations and this pair was outgroup to the C. elegans, and H. bacteriophora population and this was outgroup with A. xianyangenisis(Nguyen et al, 2007;Godjo et al, 2018;Srivastava et al, 2022). However, in the D2 -D3 area, S. feltiae and H. bacteriophora clearly distinguished themselves from S. abbasi, and similar results were obtained in the ITS region as well.…”

Steinernema shorin. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from India

“…The steinernematid nematodes collected within the present study possess infective larvae with two horn-like structures on the labial region, which is a typical trait of species of the “ bicornutum” group. Presently, this group includes 12 described species: S. riobrave Cabanillas, Poinar and Raulston 1994 (from Texas, USA); S. bicornutum Tallósi Peters and Ehlers 1995 (from Yugoslavia); S. abbasi Elawad, Ahmad and Reid 1997 (from Oman); S. ceratophorum Jian, Reid and Hunt 1997 (from Northeast China); S. pakistanense Shahina, Anis, Reid, Rowe and Maqbool 2001 (from Pakistan); S. yirgalemense Nguyen, Tesfamariam, Gozel, Gaugler and Adams 2004 (from Ethiopia); S. bifurcatum Fayyaz, Yan, Qui, Han, Gulsher, Khanum and Javed 2014 (from Pakistan); S. papillatum San-Blas, Portillo, Nermut′, Půža and Morales-Montero 2015 (from Venezuela); S. biddulphi Çimen, Půža, Nermut′, Hatting, Ramakuwela and Hazir 2016 (from South Africa); S. goweni San-Blas, Morales-Montero, Portillo, Nermut′ and Půža 2016 (from Zulia State, Venezuela); S. ralatorei Grifaldo-Alcantara, Alatorre-Rosas, Segura-León and Hernandez-Rosas 2017 (from a sugarcane area in Mexico), and S. kandii Godjo, Afouda, Baimey, Couvreur, Zadji, Houssou, Bert, Willems and Decraemer 2019 (from northern Benin).…”

First record of native entomopathogenic nematodes from Montana agroecosystems

“…Therefore, search for additional potential EPN species is being carried out in different parts of the world on a regular basis. (Malan et al, 2016;Cimen et al, 2016;Majić et al, 2018;Stock et al, 2019;Godjo et al, 2019). Indigenous EPNs have been used successfully as biological control agents to suppress various insect populations (Shapiro-Ilan et al, 2002).…”

“…The rapid killing of the insect host by EPNs and their feasibility of mass production ( Ehlers, 2001 ) has increased interest in searching for and using EPNs in integrated pest management systems ( Georgis et al, 2006 ). Therefore, search for additional potential EPN species is being carried out in different areas on a regular basis ( Cimen et al, 2016 ; Malan et al 2016 ; Majić et al, 2018 ; Godjo et al, 2019 ; Stock et al, 2019 ). Indigenous EPNs have been used successfully as biological control agents to suppress various insect populations ( Shapiro-Ilan et al, 2002 ).…”