Steinernema tbilisiensis sp. n. (Nematoda: Steinernematidae)—A New Species of Entomopathogenic Nematode from Georgia

Gorgadze, Oleg; Lortkhipanidze, Manana; Ogier, Jean-Claude; Tailliez, Patrick; Burjanadze, Medea; Microorganismes-Insectes, Interactions

doi:10.17265/2161-6256/2015.04.005

JAST-A

2015

DOI: 10.17265/2161-6256/2015.04.005

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Steinernema tbilisiensis sp. n. (Nematoda: Steinernematidae)—A New Species of Entomopathogenic Nematode from Georgia

Oleg Gorgadze

Manana Lortkhipanidze²,

Jean-Claude Ogier

et al.

Abstract: Abstract:A new species of entomopathogenic nematode (EPN), Steinernema tbilisiensis sp. n. is described. The species was isolated from soil samples of the deciduous forest located in the Tbilisi area. Morphological and morphometric data as well as phylogenetic analysis show that S. tbilisiensis sp. n. belongs to the group S. affine/intermedium. S. tbilisiensis sp. n. has been attributed to the group S. affine/intermedium on the basis of spicule and gubernaculum structure. The new species differs from other spe… Show more

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Cited by 3 publications

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“…*Gorgadze[20][21][22] and n/a: Not applicable https://doi.org/10.3923/rjp.2024.01.08 | Page 2 68-70%.…”

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confidence: 99%

Local Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae) Against the Cydalima perspectalis (Lepidoptera: Crambidae) in Georgia

Gorgadze,

Kokhia,

Lortkipanidze

et al. 2024

Res. J. Parasitol

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Backround and Objective: Cydalima perspectalis is a major invasive species of Buxus colchica common in Georgia. It was decided to investigate the mortality of C. perspectalis using local entomopathogenic nematodes (EPN). Materials and Methods:To study the efficiency of local EPNs, was invaded the pest with the above mentioned nematodes and studied their effect on them. Use of identified effective species in the field experiments. These species of nematodes were first tested on C. perspectalis. The studies were carried out both in the laboratory as well in field conditions. Results: In the laboratory experiments, two different doses of a suspension of nematodes were used: 50 and 100 IJs/against of these five species, the highest insect mortality was due to the action of Heterorhabditis sp. (100%), Steinernema gurgistana (100%) and Steinernema thesami (97.4%). These three highly virulent species were used in the field trials at doses of 1000 and 2000 Ijs LG 1 water. In this case, Heterorhabditis sp. was less effective (56.5%) than S. thesami (85.5%) and S. gurgistana (78.1%). Conclusion: Studies showed that the local species of these nematodes used in the experiments (S. thesami and S. gurgistana) are characterized by the high pathogenicity of this pest.

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“…*Gorgadze[20][21][22] and n/a: Not applicable https://doi.org/10.3923/rjp.2024.01.08 | Page 2 68-70%.…”

mentioning

confidence: 99%

Local Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae) Against the Cydalima perspectalis (Lepidoptera: Crambidae) in Georgia

Gorgadze,

Kokhia,

Lortkipanidze

et al. 2024

Res. J. Parasitol

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First report of entomopathogenic nematode Steinernema affine (Nematoda: Steinernematidae) in Syria and its virulence against Galleria mellonella L. (Lepidoptera: Pyralidae)

Mai

Allouf

Ahmad

2022

Egypt J Biol Pest Control

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Background Entomopathogenic nematodes (EPNs) have a great potential as a biocontrol agent for a wide range of insect pests. EPNs have attracted much interest around the world to study their distribution, virulence, and usage in the IPM programs. This research was conducted to identify the steinernematids M.22 and M.313, which were isolated during the survey of EPNs in different agricultural and ecological systems, in the Syrian coastal region between 2017 and 2020. Additionally, to evaluate the virulence of these isolates against the last instar larvae of the greater wax moth, Galleria mellonella (Linnaeus 1758) (Lepidoptera: Pyralidae), three bioassays were carried out at 18 °C. The exposure time assay was performed by applying different times (15, 30, 45, and 60 min) using a specified concentration (400 IJs/Larva). The concentration–response and one-on-one assays were conducted by using different concentrations of the infective juveniles (IJs) (5, 15, 25, and 40 IJs/L) and an individual juvenile, respectively, for 72 h of exposure. LC50 and LT50 values were calculated. Results The steinernematids M.22 and M.313 were isolated from 2 of 821 soil samples (0.24%). The positive isolates were identified as Steinernema affine (Bovien, 1937), based on morphological, morphometric, and molecular characterization. Steinernema affine was recovered in the walnut orchard and grassland in AL-Kadmous area of Tartus governorate, Syria. The soil type was silty loamy and silty, PH was slightly acidic, and organic matter content was 6 and 4.1%. In the exposure time assay, the mortality ratio increased gradually by increasing time, M.22 and M.313 achieved the highest mortality ratios at 60 min, which were 90.16 and 91.49%, respectively. In the concentration–response assay, complete mortality was achieved at the concentration of 40 IJs/L for both isolates. Low mortality ratios were observed in the assay of one-on-one, 20 and 23.6%, respectively. The LC50 and LT50 values were calculated and resulted in (11.7 IJs/L, 33.47 min) and (11.5 IJs/L, 32.43 min), respectively. Nonsignificant difference between both isolates was recorded. Conclusions The results showed that the isolates were identical and identified as Steinernema affine. This is the first report of S. affine in Syria. It could be an effective biological control agent.

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Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Labaude

Griffin

2018

Insects

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Entomopathogenic nematodes from the two genera Steinernema and Heterorhabditis are widely used as biological agents against various insect pests and represent a promising alternative to replace pesticides. Efficacy and biocontrol success can be enhanced through improved understanding of their biology and ecology. Many endogenous and environmental factors influence the survival of nematodes following application, as well as their transmission success to the target species. The aim of this paper is to give an overview of the major topics currently considered to affect transmission success of these biological control agents, including interactions with insects, plants and other members of the soil biota including conspecifics.

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Steinernema tbilisiensis sp. n. (Nematoda: Steinernematidae)—A New Species of Entomopathogenic Nematode from Georgia

Cited by 3 publications

References 20 publications

Local Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae) Against the Cydalima perspectalis (Lepidoptera: Crambidae) in Georgia

Local Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae) Against the Cydalima perspectalis (Lepidoptera: Crambidae) in Georgia

First report of entomopathogenic nematode Steinernema affine (Nematoda: Steinernematidae) in Syria and its virulence against Galleria mellonella L. (Lepidoptera: Pyralidae)

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

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