Stem Bark of Canarium odontophyllum Miq. (Dabai) as Potential Source of Antimicrobial Agent

Basri, Dayang Fredalina; Mustari, Noor Fazdilah; Alamin, Zafira Ayushah Zainul; Zin, Noraziah M.

doi:10.17576/jskm-2017-1501-01

Cited by 5 publications

(12 citation statements)

References 8 publications

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“…From the MTT assay, acetone extract showed a higher cytotoxic effect compared with methanol extract whereas aqueous extract was not cytotoxic at 24 h of treatment. In the previous study, the same pattern of data was seen by all three extracts but at much higher IC 50 values [ 10 ]. This may be because of the difference in the range of concentration used.…”

Section: Discussionsupporting

confidence: 78%

“…It is said that plant-derived polyphenolic compounds act as antitumor compounds and have apoptosis-inducing properties in cancer cells [ 31 ]. Based on the previous study [ 10 ], acetone extract from stem bark of C. odontophyllum was found to have phenolic compounds and other phytochemicals such as saponin, terpenoid and tannin. A study found that acetone extract from stem bark of Cephaltaxus griffithii Hook f. induced apoptosis towards HeLa cells [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…The pulp extract from C. odontophyllum fruit was found to inhibit the growth of Candida glabrata [ 9 ]. The leaf and shell extracts from C. odontophyllum were shown to have antimicrobial activity against a wide range of pathogenic bacteria [ 10 ] whereas both the leaf and stem bark of C. odontophyllum demonstrated promising anticancer property [ 11 ]. However, previous study merely reported preliminary screening of cytotoxic activity against human colorectal carcinoma HCT 116 cell line attributed to the presence of flavonoid, tannin, saponin, terpenoid and phenolic compound [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Basri¹,

Alamin²,

Chan³

2015

BMC Complement Altern Med

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Background Canarium odontophyllum Miq. is a plant species widely known as ‘dabai’ and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line.MethodThe IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200 μg/ml at treatment time of 24, 48 and 72 h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48 h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30 min of IC10 and IC25 treatment.ResultsAcetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72 h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48 h, however at 72 h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48 h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively.ConclusionsAcetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Basri¹,

Alamin²,

Chan³

2015

BMC Complement Altern Med

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“…Dabai fruit is recognized as Sibu olive because of its similar appearance, flavor, and texture with olive [ 1 ]. Interestingly, the dabai fruit fractions (pulp and seed) possess various biological activities such as antimicrobial (seed) [ 2 ], anti-fungal (pulp) [ 3 ], anti-Alzheimer’s (pulp and seed) [ 4 ], and anti-hyperglycemic (pulp) [ 5 ]. Phytochemicals identified from dabai fruit (pulp with peel), were phenolic acids (ellagic, vanillic acids, ethyl gallate), flavonoids (catechin, epicatechin, epicatechin gallate, epigallocatechin gallate, apigenin), anthocyanins (malvidin-3,5-di- O -glucoside, cyanidin-3- O -glucoside, cyanidin-3- O -rutinoside and peonidin-3- O -glucoside), and anthocyanidins (cyanidin, pelargonidin and delphinidin) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effect of Supercritical Carbon Dioxide Extracted Dabai Pulp Oil and Its Defatted Pulp

et al. 2021

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All food scientists must utilize plants for their application as functional foods to reduce hypercholesterolemia incidence through diet. Canarium odontophyllum (dabai) is a novel source for new healthy oil and functional foods. In this work, we evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO2) extracted dabai pulp oil (DPO) and defatted dabai pulp (DDP) against hypercholesterolemia elicited by a high-cholesterol diet in rats. Our results show that DPO and DDP supplementation exerted beneficial hypocholesterolemic effects against the high-cholesterol diet-fed rat. Nevertheless, supplementation with DDP revealed superior total cholesterol, low-density lipoprotein, and HMG-CoA reductase lowering efficacy (p < 0.05). Supplementation of either DPO or DDP did not significantly affect AST and ALT levels than normal rats (p > 0.05). Therefore, DDP and DPO are considered as having no toxicological significance. The histological section of rats treated with DPO and DDP showed improved steatosis in hepatocytes. HPLC analysis revealed that DPO and DDP contained syringic acid, which plays an important role in the beneficial effect. In conclusion, our results support the hypocholesterolemic and hepatoprotective effects of DPO and DDP in the hypercholesterolemic rats model.

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“…The leaves from C. odontophyllum were found to have antimicrobial activity [8]. Recently, the acetone bark extract from C. odontophyllum was also reported to demonstrate bactericidal effect against S. aureus ATCC 25923 from minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays [9]. The present study, therefore, aims to investigate the antimicrobial activity of C. odontophyllum acetone stem bark extract against the resistant strain of S. aureus, namely MRSA Mu50 strain using time-kill assay (TKA) and post-antibiotic effect (PAE) assessments.…”

Section: Introductionmentioning

confidence: 99%

Time-kill assay and post-antibiotic effect of acetone extract from the stem bark of Canarium odontophyllum against Methicillin-resistant Staphylococcus aureus Mu50 strain

2019

jabb

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Canarium odontophyllum Miq. is an indigenous fruit found in Sarawak, Malaysia. Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that causes to hospital (health-care-acquired MRSA [HA-MRSA]) and community (CA-MRSA) infections worldwide. Vancomycin has been the therapeutic drug of choice against MRSA, but unfortunately this pathogen has developed some degree of resistance to vancomycin. This research aimed to evaluate the antimicrobial activity of the stem bark extract of C. odontophyllum against MRSA Mu50 strain. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of extract and vancomycin against MRSA were determined using broth microdilution method and streak plate method. The rate of killing by the extract against Mu50 strain was determined using time-kill assay (TKA) at ×1 MIC, ×2 MIC, ×4 MIC, and ×8 MIC of the extract. The post-antibiotic effect (PAE) time of extract ×10 MIC against MRSA was also investigated. The extract exhibited bacteriostatic effect against MRSA Mu50 strain with MIC and MBC values of 1.563 mg/ml and 3.125 mg/ml, respectively. From TKA analysis, the extract was not capable of killing the Mu50 strain at ×1 MIC and ×2 MIC, but it displayed bactericidal activity at higher concentrations tested. Interestingly, the acetone stem bark extract of C. odontophyllum at ×4 MIC showed comparable time-killing kinetic with the standard antibiotic in the study. The PAE time of the extract was 3.6 ± 0.51 h against MRSA Mu50 compared to vancomycin at 2.4 ± 0.68 h. In conclusion, the stem bark acetone extract from C. odontophyllum demonstrated concentration-dependent bactericidal effect with prolonged PAE time against MRSA Mu50 strain.

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Stem Bark of Canarium odontophyllum Miq. (Dabai) as Potential Source of Antimicrobial Agent

Cited by 5 publications

References 8 publications

Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Hepatoprotective Effect of Supercritical Carbon Dioxide Extracted Dabai Pulp Oil and Its Defatted Pulp

Time-kill assay and post-antibiotic effect of acetone extract from the stem bark of Canarium odontophyllum against Methicillin-resistant Staphylococcus aureus Mu50 strain

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