Stem cell-based therapies for fertility preservation in males: Current status and future prospects

Liu, Hanchao; Xie, Yizhen; Deng, Chao; Liu, Guihua

doi:10.4252/wjsc.v12.i10.1097

Cited by 12 publications

(5 citation statements)

References 212 publications

(199 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the LCs in the organoids possessed endocrine functionality and LH responsiveness. FSH was also shown to increase the levels of Inhibin b, which may be an important factor in the self-renewal of SSCs [35]. These data demonstrate that our organoids can sustain long-term and durable endocrine function with preserved gonadotropin responsiveness, providing an effective model for studying testis endocrine function in vitro.…”

Section: Discussionmentioning

confidence: 52%

In vitro reconstitution of the hormone-responsive testicular organoids from murine primary testicular cells

et al. 2022

View full text Add to dashboard Cite

Increasing rates of male infertility require more experimental models to understand the mechanisms underlying male infertility. In vitro organoids hold unprecedented promise for this purpose; however, the development of organoids with tissue architecture similar to that of the testis in vivo remains a challenge. Here, we generated testicular organoids derived from testicular cells by combining hanging drop culture and rotation a culture system. Our results indicated that testicular cells could self-assemble into spheroid organoids with tubule-like structures in hanging drop culture. The organoids can subsequently be cultured and maintained in a rotation culture systems. These established organoids have gene expression profiles similar to those of adult testis tissue, produce testosterone with preserved gonadotropin responsiveness, and exhibit sensitivity to reproductive toxicants. More importantly, each testicular organoid can be generated from only 2000 cells, and they maintain their proliferative ability after freezing and thawing. These features make it possible to obtain fresh primary testis cells from a testicular biopsies taken from patients or endangered wild species, and to build individual-specific biobanks. These findings will help enable the exploration of self-organization process of testicular cells and provide an experimental model for reproductive biology research, pharmacotoxicology testing, and regenerative medicine.

show abstract

Section: Discussionmentioning

confidence: 52%

In vitro reconstitution of the hormone-responsive testicular organoids from murine primary testicular cells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Similar to MSC Exos, Exos isolated from induced pluripotent stem cells (iPSCs) could recover testicular function and support protective signals against damaged spermatogenesis. [90] Phosphorylation of tyrosine residues is a critical event that can control the activity of sperms and thus the fertility rate. It was found that seminal plasma Exos can phosphorylate tyrosine to improve sperm capacitation and acrosomal reaction.…”

Section: Therapeutic Effects Of Exos On the Reproductive Systemmentioning

confidence: 99%

“…Similar to MSC Exos, Exos isolated from induced pluripotent stem cells (iPSCs) could recover testicular function and support protective signals against damaged spermatogenesis. [ 90 ]…”

Section: Therapeutic Effects Of Exos On the Reproductive Systemmentioning

confidence: 99%

Exosomes as Theranostic Agents in Reproduction System

Izadpanah,

Yalameha,

Sani

et al. 2023

Advanced Biology

View full text Add to dashboard Cite

Exosomes (Exos), belonging to extracellular vesicles, are cell‐derived nano‐sized vesicles with the potential to carry different kinds of biological molecules. Many studies have proved the impacts of exosomal cargo on several biological processes in female and male reproductive systems. It is also hypothesized that changes in exosomal cargo are integral to the promotion of certain pathological conditions, thus Exos can be used as valid biomarkers for the diagnosis of infertility and other abnormal conditions. Here, efforts are made to collect some recent data related to the physiological significance of Exos in the reproductive system, and their potential therapeutic effects. It is anticipated that the current review article will lay the groundwork for elucidating the source and mechanisms by which Exos control the reproductive system additionally supplying fresh methods and concepts for the detection and treatment of disorders associated with fertility for future studies.

show abstract

“…Currently, there are not many references that exclude the possibility of reoccurrence of cancer by autotransplantation, thus re-implantation strategies for real-time clinical application are still under study. SSC cells are maintained in their natural niche and can only be proposed if the presence of tumor cells is excluded (Liu et al 2020). Xenografts have shown full spermatogenesis in several animal species but never in humans (Goossens et al 2008).…”

Section: Nanotechnology and Sperm Lifementioning

confidence: 99%

Male Fertility Preservation: A boon for young cancer survivors

2022

View full text Add to dashboard Cite

Diagnosis of any ailment especially cancer is found to be pivotal to evaluating the type of treatment that needs to be administered to man. It aids in subsequent prognosis and timely recovery in patients. When concerned with male cancer survivors, the emphasis on their fertility health is always an issue. As the numbers of survivors are increasing day by day due to the advanced medical and technological approaches, man could look with confidence to a life of ease from cancer. To review and compile all the feasible as well as relevant information about the preservation of male fertility from published resources. Reputed databases were searched for content based on specific keywords like “fertility preservation after cancer treatment", "methods of male gamete preservation", "methods of semen collection for preservation", "fertility preservation", "erectile dysfunction" and "testicular cancer and fertility". The year of publication for articles under study was restricted from 2016-2021 in most of the databases. It was found that oncologists generally recommended preservation of the male fertility before the commencement of the cancer treatment procedures. Preservation of fertility among young men should be considered in all patients before initiating any kind of prognosis related to the disease.

show abstract

Stem cell-based therapies for fertility preservation in males: Current status and future prospects

Cited by 12 publications

References 212 publications

In vitro reconstitution of the hormone-responsive testicular organoids from murine primary testicular cells

In vitro reconstitution of the hormone-responsive testicular organoids from murine primary testicular cells

Exosomes as Theranostic Agents in Reproduction System

Male Fertility Preservation: A boon for young cancer survivors

Contact Info

Product

Resources

About