Stem cell-delivery therapeutics for periodontal tissue regeneration

Chen, Fa‐Ming; Sun, Hao; Lü, Hong; Yu, Qing

doi:10.1016/j.biomaterials.2012.05.048

Cited by 266 publications

(260 citation statements)

References 201 publications

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“…In this context, dental tissues have also been investigated as niches of MSCs, and many toothderived SCs (TDSCs) have been identified and characterized, including dental pulp SCs (DPSCs) [44][45][46][47][48] , SCs from human exfoliated deciduous teeth (SHED) [49][50][51][52][53] , periodontal ligament SCs (PDLSCs), dental follicle progenitor cells (DFPCs) [54][55][56] , SCs from apical papilla (SCAP) [19,[56][57][58][59] , and periodontal ligament of deciduous teeth SCs (DePDL) [50,51,[60][61][62] (Figure 1). Dental tissues are an accessible source of MSCs that can be obtained with limited morbidity and without additional risks to the donor, as extracted/exfoliated teeth represent a waste product of dental procedures [13,[63][64][65] . However, the properties of these TDSCs and their feasibility for regenerating tissues still need to be investigated in greater detail.…”

Section: Introductionmentioning

confidence: 99%

Tooth-derived stem cells: Update and perspectives

Saito¹,

Silvério²,

Casati³

et al. 2015

WJSC

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Section: Introductionmentioning

confidence: 99%

Tooth-derived stem cells: Update and perspectives

Saito¹,

Silvério²,

Casati³

et al. 2015

WJSC

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“…[5][6][7] Previous studies have indicated that the regenerative capacity of PDL is closely associated with mesenchymal stem cells (MSCs) that have been recognized to reside within human PDL tissue. 8,9 PDL stem cells (PDLSCs) represent a population of somatic stem cells of mesenchymal origin, and they were identified as MSCs in 2004. 10 PDLSCs are committed to several development lineages, and possess an ability for self-renewal and multipotent differentiation, such as osteoblasts, adipocytes, neurocytes and chondrocytes, 11,12 and play an important role in maintaining homeostasis and promote the regeneration of periodontal tissue.…”

Section: Introductionmentioning

confidence: 99%

IL-7 suppresses osteogenic differentiation of periodontal ligament stem cells through inactivation of mitogen-activated protein kinase pathway

Cao

Fan²,

Yonghe³

et al. 2016

Organogenesis

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ABSTRACT. Periodontal ligament stem cells (PDLSCs) are tissue-specific mesenchymal stem cells (MSCs), having an important role in regenerative therapy for teeth loss. Interleukin-7 (IL-7) is a key cytokine produced by stromal cells including MSCs, and exhibits specific roles for B and T cell development and osteoblasts differentiation of multiple myeloma. However, the effect of IL-7 on osteogenic differentiation of PDLSCs remains unclear. Therefore, in the present study we determined whether IL-7 affects the proliferation and osteogenic differentiation of PDLSCs in vitro and explored the associated signaling pathways for IL-7-mediated cell differentiation. The results demonstrated that the isolated human PDLSCs possessed MSCs features, highly expressing CD90, CD44, CD105, CD29 and CD73, and almost did not expressed CD34, CD45, CD11b, CD14 and CD117. IL-7 could not significantly affect the proliferation of PDLSCs, but it decreased their osteogenic differentiation and inhibited alkaline phosphatase (ALP) activity. The results of quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) and Western blotting exhibited that the expression levels of Runx-2, SP7 and osteocalcin (OCN) were significantly reduced by IL-7. Further studies indicated that IL-7 did not significantly change JNK, ERK1/2 and p38 protein production, but markedly suppressed their phosphorylation levels. These data suggest that IL-7 inhibits the osteogenic differentiation of PDLSCs probably via inactivation of mitogen-activated protein kinase (MAPK) signaling pathway.

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“…To further increase the effectiveness of this methodology, the use of different scaffolds in association with stem cells with osteoblast-like activity has been introduced [2,3]. Mesenchymal stem cells (MSCs) have been proved to be effective with such developed biomaterials, since they are capable to induce new bone formation in critical-size defects of animal models after differentiation towards osteoblastic phenotype [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Histomorphometric Evaluation of Bone Regeneration Induced by Biodegradable Scaffolds as Carriers for Dental Pulp Stem Cells in a Rat Model of Calvarial "Critical Size" Defect

Annibali¹,

Quaranta²,

Scarano³

2016

J Stem Cell Res Ther

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Objective: The aim of this study was to test specific stem cells that could enhance bone formation in combination\ud with specific scaffolds.\ud Methods: Dental Pulp Stem Cells (DPSCs) were seeded with Granular Deproteinized Bovine Bone (GDPB) or Beta-Tricalcium Phosphate (ß-TCP) in a rat model of calvarial "critical size" defect. DPSCs were isolated from permanent human teeth, obtained and characterized using specific stem cells markers (Nanog and Oct-4) by real time-PCR and immunofluorescence. Cells were differentiated for 10-15 days towards the osteoblastic phenotype with 100μM L-ascorbic acid, added every day in culture medium and 20 vol. percentage of FBS in α-MEM medium. Osteogenic commitment was evaluated with real time-PCR by measuring the expression of specific markers (osteonectin and runx2). When a sufficient cell number was obtained, DPSCs were trypsinized, washed in culture medium and seeded onto the GDPB and ß-TCP scaffold sat a density of 0.5-1×106 cells/scaffold. Two bilateral critical-size circular defects (5 mm diameter; 1 mm thickness) were created from the parietal bone of the 8 athymic T-cell deficient nude rats. One cranial defect for each rat was filled with the scaffold alone and the other defect with the scaffold seeded with stem cells. After 12 weeks post-surgery animals were euthanized and histomorphometric analysis was performed. Differences between groups were analyzed by one-way analysis of variance (ANOVA) followed by Fisher's Protected Least Significant Difference (PLSD) post-hoc test. A p-value <0.05 was considered statistically significant.\ud Results: GDPB group presented higher percentage of lamellar bone than that of GDPB/DPSC, ß-TCP alone had lower levels as compared to ß-TCP/DPSC. The addition of stem cells significantly increased woven bone formation in both scaffold-based implants, although still higher in GDPB based implants.\ud Conclusion: Our findings indicate that GDPB and ß-TCP used as scaffold to induce bone regeneration may benefit from adding DPSC to tissue-engineered constructs

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Stem cell-delivery therapeutics for periodontal tissue regeneration

Cited by 266 publications

References 201 publications

Tooth-derived stem cells: Update and perspectives

Tooth-derived stem cells: Update and perspectives

IL-7 suppresses osteogenic differentiation of periodontal ligament stem cells through inactivation of mitogen-activated protein kinase pathway

Histomorphometric Evaluation of Bone Regeneration Induced by Biodegradable Scaffolds as Carriers for Dental Pulp Stem Cells in a Rat Model of Calvarial "Critical Size" Defect

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