Stem cell differentiation with consistent lineage commitment induced by a flash of ultrafast-laser activation in vitro and in vivo

Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser

“…Insert: the mice on the stage with anesthesia during the photoactivation experiment. This figure was reused from Tang et al. (2022) .…”

“…The differentiation of those photoactivated BCs to club cells could be finally induced in the in vivo in the damaged airway environment. The mechanism of this protocol is, as we proposed, the pathways ERK and Wnt/β-catenin initiated simultaneously by Ca 2+ and weak photodamage elicit the laser-induced differentiation ( Tang et al., 2022 ). It could be expected this method could probably work on differentiation of various types of stem cells if their differentiation is related to these two pathways.…”

“…This protocol also works for human umbilical cord mesenchymal stem cells (hUC-MSCs) and GNPs in vitro . According to the mechanism of this protocol we proposed, the pathways ERK and Wnt/β-catenin initiated simultaneously by Ca 2+ and weak photodamage mediated the photoactivated differentiation ( Tang et al., 2022 ). Theoretically, this method could be applied to stem cell types that share similar differentiation pathways.…”

“…This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo . For complete details on the use and execution of this protocol, please refer to Tang et al. (2022) .…”

“…For complete details on the use and execution of this protocol, please refer to Tang et al. (2022) .…”