Stem cell therapy serves as effective treatment for tissue regeneration. Nevertheless, stem cells from bone marrow and peripheral blood are still lacking autologous and developmental homologous properties. Dental pulp stem cells (DPSCs) from autologous deciduous tooth are derived from neural crest, in coincidence with maxillofacial tissues, thus attracting great interest in in situ maxillofacial regenerative medicine. However, insufficient number of seed cells and heterogenous alteration of cell phenotypes retard further exploration of DPSC‐based tissue engineering. Electric stimulation has recently attracted great interest in tissue regeneration. In this study, we fabricated a novel DPSC‐loaded conductive hydrogel microspheres integrated with wireless electric generator. Application of exogenous electric cues can promote stemness maintaining and heterogeneity suppression for unpredictable differentiation of encapsulated DPSCs. Further investigations observed that electric signal fine‐tunes regenerative niche by improvement on DPSC‐mediated paracrine pattern, evidenced by enhanced angiogenic behavior and upregulated anti‐inflammatory macrophage polarization. By wireless electric stimulation on implanted conductive hydrogel microspheres, loaded DPSCs facilitates the construction of immuno‐angiogenic niche at early stage of tissue repair, and further contributes to advanced autologous mandibular bone defect regeneration. Overall, a conductive microsphere is provided as stem cell carrier that facilitates the stemness maintaining and paracrine behavior of DPSCs for modulation on regenerative niche. Application of integrated charging‐conducting cell carrier on DPSC‐based tissue engineering exhibits promising translational and therapeutic potential for autologous maxillofacial tissue regeneration, and paves the way for autologous and homologous stem cell‐based tissue engineering.This article is protected by copyright. All rights reserved
