Stem Cell Therapies for Tissue Regeneration and Wound Healing: Strategies to Enhance Therapeutic Effectiveness

Mihalceanu, Silvia; Aitzetmüller, Matthias M.; Machens, Hans‐Günther; Duscher, Dominik

doi:10.1007/978-3-030-19958-6_18

Cited by 3 publications

(2 citation statements)

References 100 publications

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“…Im Gegensatz zu den oben genannten klinischen Parametern hängt die Überlebensrate des Fett-Grafts zu einem großen Teil von dem Überleben der im Fett enthaltenen adipogenen Stammzellen (= Adipose-derived Stem Cells = ADSCs) ab. Diese wirken einerseits über die Sekretion von pro-regenerativen Zytokinen, die das Überleben der benachbarten Zellen positiv beeinflussen und andererseits über asymmetrische Teilung (Differenzierung) zu benötigten Zelltypen [11]. Grundsätzlich…”

Section: Grundlagenunclassified

Der Einfluss von Gewinnung und Verarbeitung auf das Regenerationspotenzial von Fettstammzellen und die Adipozytenvitalität

Klietz

Kückelhaus

Wiebringhaus

et al. 2021

Handchir Mikrochir Plast Chir.

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ZusammenfassungDer autologe Lipotransfer stellt eine etablierte Innovation der Plastischen Chirurgie dar. Als Methode zur Mammarekonstruktion und -augmentation bietet er eine klinisch immer bedeutender werdende Alternative zu den konventionellen Verfahren wie der Lappenchirurgie oder der Verwendung von Silikonimplantaten.Der Erfolg oder Misserfolg des autologen Lipotransfers hängt größtenteils vom Überleben der im Fett befindlichen Fettstammzellen ab.Diese Überlebensrate ist wiederum von einem komplexen Zusammenspiel der Zwischenschritte bei der Fettabsaugung und potenzieller Aufbereitung des Fettgewebes abhängig.Dieses Review beleuchtet die entscheidenden Prinzipien zur Erzielung optimaler Ergebnisse.

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Section: Grundlagenunclassified

Der Einfluss von Gewinnung und Verarbeitung auf das Regenerationspotenzial von Fettstammzellen und die Adipozytenvitalität

Klietz

Kückelhaus

Wiebringhaus

et al. 2021

Handchir Mikrochir Plast Chir.

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“…An increasing amount of effort has been made to develop stem cell-based therapies for tissue regeneration. 9 Transplantation of endogenous and exogenous stem cells, which has been widely explored, is not available clinically because of limited cell lifespan, immune rejection, tumorigenic potential, and pathogen transfer. 10 To overcome these limitations, our laboratory was the first to develop scaffolds capable of triggering autologous stem cell responses.…”

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Biomolecule‐releasing bioadhesive for glenoid labrum repair through induced host progenitor cell responses

Nguyên

Vaish

et al. 2022

Journal Orthopaedic Research

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Glenoid labral tears occur with repetitive dislocation events and are common injuries observed in shoulder arthroscopic procedures. Although surgery can restore shoulder anatomy, repair is associated with poor clinical outcomes, which may be attributed to the poor regenerative capability of glenoid labral fibrocartilage. Thus, this study was designed to assess whether in situ tissue regeneration via biomolecule‐stimulated recruitment of progenitor cells is a viable approach for the regeneration of labral tears. We developed a click chemistry‐based bioadhesive to improve labral repair and reduce local inflammatory responses due to trauma. Additionally, we previously identified the presence of progenitor cells in the human labrum, which can be recruited by platelet‐derived growth factor (PDGF). Thus, we hypothesized that PDGF‐releasing adhesives could induce the regenerative responses of progenitor cells at the injury site to improve labral healing. In a rat glenoid labral tear model, we evaluated the effect of PDGF‐releasing adhesives on promoting progenitor cells to participate in labral tear healing. After 3 and 6 weeks, the labrum was histologically analyzed for inflammatory responses, progenitor cell recruitment, proliferation, and extracellular matrix (ECM) production (collagen and glycosaminoglycan). Our results showed that adhesives alone considerably reduced local inflammatory responses and labral tissue dissolution. PDGF‐releasing adhesives significantly increased progenitor cell recruitment, proliferation, and ECM production. These results demonstrate that by accelerating autologous progenitor cell responses, PDGF‐releasing adhesives represent a novel clinically relevant strategy to improve the healing of glenoid labral tears.

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Erythropoetin can partially restore cigarette smoke induced effects on Adipose derived Stem Cells

Kükrek

Aitzetmüller

Vodiškar

et al. 2021

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INTRODUCTION: Adipose derived Stem Cells (ASCs) have been proven to play a key role in tissue regeneration. However, exposure to large amounts of cigarette smoke can drastically diminish their function. Erythropoetin (EPO), can modulate cellular response to injury. Therefore, we investigated the ability of EPO to restore the regenerative function and differentiation capacity of ASCs. MATERIAL AND METHODS: Human ASCs were isolated from abdominoplasty samples using standard isolation procedures. Cell identity was established by means of Fluorescence Activated Cell Scanning. Subsequently, isolated ASCs were cultivated with cigarette smoke extract both with and without EPO. Parameters investigated included cellular metabolic activity, adipogenic and osteogenic differentiation capacity, and in vitro wound closure capacity. For further enhancing wound closure, EPO was combined with Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) or Stromal Derived Factor-1 alpha (SDF-1 a). RESULTS: Cigarette smoke reduces adipogenic differentiation, the osteogenic differentiation capacity as well as the in vitro wound healing ability of human derived ASCs. EPO did not change metabolic activity of ASCs significantly. The addition of EPO could partially restore their function. The combination of EPO with GM-CSF or SDF-1 did not result in a synergistic effect regarding wound healing ability. CONCLUSION: Exposure to cigarette smoke significantly reduced the regenerative potential of ASCs. Treatment of ASCs exposed to cigarette smoke with EPO has the potential to partially restore their function.

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Stem Cell Therapies for Tissue Regeneration and Wound Healing: Strategies to Enhance Therapeutic Effectiveness

Cited by 3 publications

References 100 publications

Der Einfluss von Gewinnung und Verarbeitung auf das Regenerationspotenzial von Fettstammzellen und die Adipozytenvitalität

Der Einfluss von Gewinnung und Verarbeitung auf das Regenerationspotenzial von Fettstammzellen und die Adipozytenvitalität

Biomolecule‐releasing bioadhesive for glenoid labrum repair through induced host progenitor cell responses

Erythropoetin can partially restore cigarette smoke induced effects on Adipose derived Stem Cells

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