Stem cells and endometrial hyperplasia

Abstract

Endometrial hyperplasia is characterized by a proliferation of endometrial glands that may progress to or coexist with endometrial carcinoma. Previous studies proved the presence of endometrial stem cells that may have a role in endometrial regeneration and may be responsible for proliferative disorders as in endometrial hyperplasia and endometrial carcinoma. The presentstudy carried out to evaluate the potentiality of Umbilical cord blood Hematopoietic CD34+ and Mesenchymal stem cells to regress endometrial hyperplastic cells proliferation in vitro under certain condition. The Study carried out on 40 Umbilical cord blood samples collected from term delivering women, mononuclear cell were separated. Endometrial samples from the 40 cases (20normal and 20 cases with endometrial hyperplasia) were collected by dilatation and curettage or by curetting endometrium after hysterectomy operation. Then treatment of the hyperplastic cells with the Mesenchymal stem cells extract and CD34+ Hematopoietic stem cells extract was done. Results showed that both CD34+ Hematopoietic stem cells extract and mesenchymal stem cellsextract both had decreased the rate of growth of endometrial hyperplastic cell culture reaching near to the rate of growth of normal endometrial primary cell culture but the mesenchymal stem cells extract had a higher degree of control making endometrial growth rate nearer to the rate of growth of normal endometrial primary cell culture.