Stem cells derived from “inflamed” and healthy periodontal ligament tissues and their sheet functionalities: a patient‐matched comparison

Tang, Hao-Ning; Xia, Yu; Yu, Yang; Wu, Ruixue; Gao, Lina; Chen, Fa‐Ming

doi:10.1111/jcpe.12501

Cited by 83 publications

(87 citation statements)

References 46 publications

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“…The combined data suggest that cell responses to a hypoxic stimulus may vary from cell type to cell type. While previous work indicated that an inflammatory stimulus causes an increased proliferation rate of PDLSCs Tang et al 2016;Zheng et al 2015), such an increase has not been detected in this particular research; our data suggest that the 24-h inflammatory pretreatment has no obvious effect on PDLSC colony formation and proliferation (Fig. 3).…”

Section: Discussioncontrasting

confidence: 81%

“…In parallel, methods for PDLSC isolation and production are developing very quickly, from rapid and safe cell propagation/expansion (Iwata et al 2010;Jung et al 2013;Trubiani et al 2015;Wu et al 2016) to optimal cell material production (Wei et al 2012;Gao et al 2013;Iwata et al 2015). Nevertheless, major challenges still exist, particularly challenges related to producing large numbers of cells from clinically limited PDL tissues and manufacturing/banking robust cell materials, ideally in an off-the-shelf format Tang et al 2016;Wu et al 2016). Great efforts have been and are still being made in this arena to either avoid long-term ex vivo manipulation, maintain cell properties during extensive ex vivo handling and/or modify/engineer cell behavior toward a target purpose (Sharma et al 2011;Thirumala et al 2013).…”

Section: Discussionmentioning

confidence: 97%

“…Despite being a promising stem cell reservoir, the generation of sufficient amounts of stem cells from the limited PDL tissues remaining on the root surface of a removed tooth remains an elusive goal Tang et al 2016); therefore, long-term ex vivo culture and expansion to reach the required population size are necessary. However, PDLSC proliferation remains slow under the current culture conditions.…”

Section: Introductionmentioning

confidence: 99%

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Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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In this study, we extensively screened the in vitro and in vivo effects of PDLSCs following short-term inflammatory and/or hypoxic pretreatments. We found that the 24-h hypoxic pretreatment of PDLSCs significantly enhanced cell migration and improved cell surface CXCR4 expression. In addition, hypoxia-pretreated PDLSCs exhibited improved cell colony formation and proliferation. Cells that were dually stimulated also formed more colonies compared to untreated cells but their proliferation did not increase. Importantly, the hypoxic pretreatment of PDLSCs enhanced cell differentiation as determined by elevated RUNX-2 and ALP protein expression. In this context, the inflammatory stimulus impaired cell OCN protein expression, while dual stimuli led to decreased RUNX-2 and OCN mRNA levels. Although preconditioning PDLSCs with inflammatory and/or hypoxic pretreatments resulted in no differences in the production of matrix proteins, hypoxic pretreatment led to the generation of thicker cell sheets; the inflammatory stimulus weakened the ability of cells to form sheets. All the resultant cell sheets exhibited clear bone regeneration following ectopic transplantation as well as in periodontal defect models; the amount of new bone formed by hypoxia-preconditioned cells was significantly greater than that formed by inflammatory stimulus- or dual-stimuli-treated cells or by nonpreconditioned cells. The regeneration of new cementum and periodontal ligaments was only identified in the hypoxia-stimulus and no-stimulus cell groups. Our findings suggest that PDLSCs that undergo short-term hypoxic pretreatment show improved cellular behavior in vitro and enhanced regenerative potential in vivo. The preconditioning of PDLSCs via combined treatments or an inflammatory stimulus requires further investigation.

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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“…The effects of different pretreatments on cell immunomodulatory activity were evaluated by detecting the proliferation/apoptosis rate of peripheral blood mononuclear cells (PBMNCs). After co‐culture with pretreated cells, PBMNCs were subjected to analysis of the proliferation/apoptosis rate; the methods for this analysis were described in detail previously . Briefly, density‐gradient centrifugation was used to extracted PBMNCs from fresh blood derived from three systemically healthy donors.…”

Section: Methodsmentioning

confidence: 99%

Hypoxia and low‐dose inflammatory stimulus synergistically enhance bone marrow mesenchymal stem cell migration

Yin

et al. 2016

Cell Proliferation

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Objectives: Cell migration is necessary for numerous physiological cell processes.Although either inflammatory or hypoxic stimuli of certain dose and duration have positive influence on cell migration, their combination has not been shown to result in a synergistic effect. Materials and methods:In this study, we investigated combined effects of hypoxia and low-dose inflammatory stimulus (one-tenth of that of a previously used concentration) on migration of human bone marrow-derived mesenchymal stem cells (BMMSCs). Results:Our results from real-time PCR, Western blot analysis and an immunofluorescence assay, showed that dual stimulation up-regulated CXCR4 expression. Based on tablet scratch experimentation and transwell assay, the dual stimuli exhibited greater positive effects on cell migration than a single inflammatory or hypoxic stimulus. When effects of various pre-treatments on cell proliferation, differentiation and immunosuppression were screened, cells subjected to the hypoxic stimulus or dual stimuli had increased cell proliferation, while short-term inflammatory stimulus and/or hypoxic stimulus had no negative effect on cell differentiation and immunosuppression.Conclusions: These findings suggest that the combination of hypoxia and low-dose inflammatory stimuli enhances the potential of BMMSCs to migrate, thus identifying cell pre-treatment conditions that could enhance future stem cell-based therapeutics.

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“…The cell‐to‐cell coordination process required to regenerate the lost periodontium needs stem cell populations that have not been altered by the inflammatory processes that originally destroyed the tissues. Indeed, it has been reported that stem cells from inflamed sites behave differently to those obtained from healthy sites . Regenerative interventions should only be attempted after a health‐associated homeostasis between the host and the resident microbiota has been restored.…”

Section: Future Challengesmentioning

confidence: 99%

A brief history of periodontics in the United States of America: Pioneers and thought‐leaders of the past, and current challenges

Armitage

2019

Periodontology 2000

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This paper summarizes historical events in periodontology in the United States over the past 200 years. The contributions of some of the key thought‐leaders of the past are highlighted. Throughout the 20th century, the evolution of thought, leading to the views currently held regarding the pathogenesis and treatment of periodontal diseases, was significantly influenced by: (1) major changes in health‐care education; (2) the emergence of periodontics as a specialty of dentistry; (3) the publication of peer‐reviewed journals with an emphasis on periodontology; (4) formation of the National Institute of Dental and Craniofacial Research (NIDCR); and (5) expansion of periodontal research programs by the NIDCR. The two major future challenges facing periodontal research are development of a better understanding of the ecological complexities of host‐microbial interactions in periodontal health and disease, and identification of the relevant mechanisms involved in the predictable regeneration of damaged periodontal tissues.

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Stem cells derived from “inflamed” and healthy periodontal ligament tissues and their sheet functionalities: a patient‐matched comparison

Abstract: These data provide additional evidence that I-PDLSCs are functionally compromised compared with H-PDLSCs. Nonetheless, their dominant abundance in the available tissues indicates that stem cells derived from damaged teeth extracted due to periodontitis warrant further exploration.

Cited by 83 publications

References 46 publications

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Hypoxia and low‐dose inflammatory stimulus synergistically enhance bone marrow mesenchymal stem cell migration

A brief history of periodontics in the United States of America: Pioneers and thought‐leaders of the past, and current challenges

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