Stem cells derived from testis show promise for treating a wide variety of medical conditions

Nayernia, Karim

doi:10.1038/cr.2007.96

Cited by 15 publications

(13 citation statements)

References 13 publications

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“…New research has shown the derivation of pluripotent stem cells from neonatal and adult mouse testis that can differentiate to all cell types (Guan et al 2007;Nayernia 2007). These studies prove that ES-like cells were generated without genetic manipulation but under specific culture conditions (De Rooij and Mizrak 2008).…”

Section: Discussionmentioning

confidence: 99%

Alteration in genes expression patterns during in vitro differentiation of mouse spermatogonial cells into neuroepithelial-like cells

et al. 2012

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Pluripotent stem cells derived from testis is a new, natural, and unlimited source for cell therapy in regenerative medicine and represent a possible alternative to replacing of all cells in the body. Here, we designed a simple co-culture system of spermatogonia cells with Sertoli cells for the generation of embryonic stem-like cells from mouse testis. The importance of our simple method will be clear when we compared it with other complex and time-consuming methods. Embryonic stem-like colonies with sharp border confirmed by real-time PCR, immunocytochemistry and flow cytometry assessments. Embryonic stem-like colonies were immunopositive for pluripotency markers. Transition of spermatogonia cells to embryonic stem-like cells was accompanied by extensive changes in gene expression. These changes included significant increase in pluripotency genes expression and significant decrease in germ cellspecific genes expression. Also, we proved the differentiation capacity of embryonic stem-like cells to neuroepithelial-like cells which were immunoreactive to Nestin and Neurofilament 68. Evaluation of genes expression during in vitro differentiation into neuroepithelial-like cells showed high-level expression of Nestin whether this gene approximately has no expression in undifferentiated embryonic stem-like cells. Also, expression of pluripotency genes has significantly decreased in neuroepithelial-like cells compared with embryonic stem-like cells. This study shows that embryonic stem-like cells derived from testis are capable to differentiate into neuroepitheliallike cells that may provide a cellular reservoir usable for neurodegenerative disorders.

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Section: Discussionmentioning

confidence: 99%

Alteration in genes expression patterns during in vitro differentiation of mouse spermatogonial cells into neuroepithelial-like cells

et al. 2012

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“…The ability of SSCs to transdifferentiate into mature hepatocytes is quite remarkable, illustrating the high plasticity of these unipotent stem cells, capable of reverting to pluripotency under specific conditions, representing potential clinical use in the treatment of liver diseases (Zhang et al 2013b). SSCs were proposed to have two potential therapeutic uses, one as a source of reprogrammable pluripotent stem cells that could be used for tissue regeneration (Nayernia 2007), the other as a reservoir of stem cells for the regeneration of spermatogenesis following cancer therapies (Valli et al 2014).…”

Section: Ra-induced Decreases In Mirc1 and Mirc3 In Thy1mentioning

confidence: 99%

Mammalian gonocyte and spermatogonia differentiation: recent advances and remaining challenges

Manku¹,

Culty²

2015

Reproduction

127

105

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The production of spermatozoa relies on a pool of spermatogonial stem cells (SSCs), formed in infancy from the differentiation of their precursor cells, the gonocytes. Throughout adult life, SSCs will either self-renew or differentiate, in order to maintain a stem cell reserve while providing cells to the spermatogenic cycle. By contrast, gonocytes represent a transient and finite phase of development leading to the formation of SSCs or spermatogonia of the first spermatogenic wave. Gonocyte development involves phases of quiescence, cell proliferation, migration, and differentiation. Spermatogonia, on the other hand, remain located at the basement membrane of the seminiferous tubules throughout their successive phases of proliferation and differentiation. Apoptosis is an integral part of both developmental phases, allowing for the removal of defective cells and the maintenance of proper germ-Sertoli cell ratios. While gonocytes and spermatogonia mitosis are regulated by distinct factors, they both undergo differentiation in response to retinoic acid. In contrast to postpubertal spermatogenesis, the early steps of germ cell development have only recently attracted attention, unveiling genes and pathways regulating SSC self-renewal and proliferation. Yet, less is known on the mechanisms regulating differentiation. The processes leading from gonocytes to spermatogonia have been seldom investigated. While the formation of abnormal gonocytes or SSCs could lead to infertility, defective gonocyte differentiation might be at the origin of testicular germ cell tumors. Thus, it is important to better understand the molecular mechanisms regulating these processes. This review summarizes and compares the present knowledge on the mechanisms regulating mammalian gonocyte and spermatogonial differentiation.

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“…In fact, these multipotent cells resemble embryonic stem (ES) cells in their differentiation capacity and in the morphology and growth characteristics of the colonies they form in culture, and, as such, in this review they will be referred to as ES-like cells. These findings have brought excitement and enthusiasm to the field, and several reports have commented on the possible uses of these SSC-derived ES-like cells, including in regenerative medicine (Cyranoski, 2006;de Rooij, 2006;Kanatsu-Shinohara and Shinohara, 2006;Nayernia, 2007;Nayernia, 2008). However, the ways in which SSCs are transformed into ES-like cells and how these cells are induced to differentiate differ considerably between the various groups that are studying SSCs.…”

Section: Introductionmentioning

confidence: 99%

Deriving multipotent stem cells from mouse spermatogonial stem cells: a new tool for developmental and clinical research

Rooij

Mizrak

2008

Development

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In recent years, embryonic stem (ES) cell-like cells have been obtained from cultured mouse spermatogonial stem cells (SSCs). These advances have shown that SSCs can transition from being the stem cell-producing cells of spermatogenesis to being multipotent cells that can differentiate into derivatives of all three germ layers. As such, they offer new possibilities for studying the mechanisms that regulate stem cell differentiation. The extension of these findings to human SSCs offers a route to obtaining personalized ES-like or differentiated cells for use in regenerative medicine. Here, we compare the different approaches used to derive ES-like cells from SSCs and discuss their importance to clinical and developmental research.

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Stem cells derived from testis show promise for treating a wide variety of medical conditions

Cited by 15 publications

References 13 publications

Alteration in genes expression patterns during in vitro differentiation of mouse spermatogonial cells into neuroepithelial-like cells

Alteration in genes expression patterns during in vitro differentiation of mouse spermatogonial cells into neuroepithelial-like cells

Mammalian gonocyte and spermatogonia differentiation: recent advances and remaining challenges

Deriving multipotent stem cells from mouse spermatogonial stem cells: a new tool for developmental and clinical research

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