Stem cells for organoids

Qian, Shutong; Mao, Jiayi; Liu, Zhimo; Zhao, Binfan; Zhao, Qing; Lu, Bolun; Zhang, Liucheng; Mao, Xiyuan; Cheng, Liying; Cui, Wenguo; Zhang, Yuguang; Sun, Xiaoming

doi:10.1002/smmd.20220007

Cited by 21 publications

(21 citation statements)

References 140 publications

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“…Immunohistochemistry: According to the method reported previously, the immunohistochemistry of the proximal left tibias after decalcification and the colons of rats were performed and assessed. [51,56,57] In brief, the sections were incubated with NLRP3 (1:200, Servicebio, China), Caspase-1 (1:300, Servicebio, China), IL-1𝛽 (1:100, Servicebio, China), IL-18 (1:200, Servicebio, China), Occludin (1: 600, Servicebio, China), and ZO-1 (1: 600, Servicebio, China), respectively. All images were captured by a LSCM (Zeiss, Germany).…”

Section: Methodsmentioning

confidence: 99%

Neuropeptide Y‐Mediated Gut Microbiota Alterations Aggravate Postmenopausal Osteoporosis

Chen,

Lv,

Liang

et al. 2023

Advanced Science

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Postmenopausal osteoporosis (PMO) is often accompanied by neuroendocrine changes in the hypothalamus, which closely associates with the microbial diversity, community composition, and intestinal metabolites of gut microbiota (GM). With the emerging role of GM in bone metabolism, a potential neuroendocrine signal neuropeptide Y (NPY) mediated brain‐gut‐bone axis has come to light. Herein, it is reported that exogenous overexpression of NPY reduced bone formation, damaged bone microstructure, and up‐regulated the expressions of pyroptosis‐related proteins in subchondral cancellous bone in ovariectomized (OVX) rats, but Y1 receptor antagonist (Y1Ra) reversed these changes. In addition, it is found that exogenous overexpression of NPY aggravated colonic inflammation, impaired intestinal barrier integrity, enhanced intestinal permeability, and increased serum lipopolysaccharide (LPS) in OVX rats, and Y1Ra also reversed these changes. Most importantly, NPY and Y1Ra modulated the microbial diversity and changed the community composition of GM in OVX rats, and thereby affecting the metabolites of GM (e.g., LPS) entering the blood circulation. Moreover, fecal microbiota transplantation further testified the effect of NPY‐mediated GM changes on bone. In vitro, LPS induced pyroptosis, reduced viability, and inhibited differentiation of osteoblasts. The study demonstrated the existence of NPY‐mediated brain‐gut‐bone axis and it might be a novel emerging target to treat PMO.

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Section: Methodsmentioning

confidence: 99%

Neuropeptide Y‐Mediated Gut Microbiota Alterations Aggravate Postmenopausal Osteoporosis

Chen,

Lv,

Liang

et al. 2023

Advanced Science

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“…Nowadays, engineered microbes have served as significant therapeutic agents for various therapeutic applications [113][114][115][116][117]. Several internal and external stimuli can activate the colonization and payload release of these administered bacteria [118,119].…”

Section: Engineered Bacteriamentioning

confidence: 99%

Emerging diagnostic and therapeutic technologies based on ultrasound-triggered biomaterials

et al. 2023

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Ultrasound (US) is a kind of acoustic waves with frequency higher than 20 kHz. Learning from the echo detection ability of bats and dolphins, scientists applied US for clinical imaging by sending out US waves and detecting echo with shifted intensities and frequencies from human tissue. US has long been played a critical role in noninvasive, real-time, low cost and portable diagnostic imaging. With the in-depth study of US in multidisciplinary fields, US and US-responsive materials has shown practical value in not only disease diagnosis, but also disease treatment. In this review, we introduce the lately proposed and representative US-responsive materials for biomedical applications, including diagnostic and therapeutic applications. We focused on US-mediated physiochemical therapies, such as sonodynamic therapy, high-intensity focused US ablation, sonothermal therapy, thrombolysis, etc., and US-controlled delivery of chemotherapeutics, gas, gene, protein, and bacteria. We also conclude the current challenges facing the clinical translation of the smart US-responsive materials and prospect future development of US medicine.

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“…Not only that, but iPSC can also avoid the ethical problems of ESC extraction from patients. [86] In the last decade or so, with the breakthrough discovery of iPSCs, researchers have differentiated them into a variety of cells with different functions and phenotypes. iPSC gradually showed great potential for applications in the direction of drug testing, disease modeling, and regenerative medicine [87,88] (Figure 7D, E).…”

Section: From Scalable Ipsc To Bone Organoidmentioning

confidence: 99%

“…[104] In contrast to iPSC, ASC-derived organoids are mainly composed of mature cells, which tend to maintain high genetic stability after long-term culture. [86] However, the differentiation potential of ASC is inferior to that of iPSC, and the derived organoids are often only transformed into a single epithelial cell type. Boonekamp et al [105] used epithelial stem cells, determined optimal culture conditions, and successfully established a human epidermal organoid model (Figure 10Ci) with the promotion of cytokines such as fibroblast growth factor (FGF).…”

Section: From Multidirectional Differentiated Adult Stem Cells To Bon...mentioning

confidence: 99%

A Review of Advanced Biomaterials and Cells for the Production of Bone Organoid

Zhang

et al. 2023

Small Science

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Rapid advancements in traditional bone tissue engineering have led to innovation in bone repair models and the resolution of insurmountable clinical issues like graft scarcity. The pathophysiological process of treating bone disease, however, is a multidimensional and multimodal regenerative regulatory mechanism that includes numerous immune, inflammatory, or metabolic responses related to the graft or the organism itself. Based on a 3D in vitro cell culture system that is remarkably identical to the body's bone tissue, the bone organoid is a biomimicking bone organ environment. It can accurately mimic the actual repair and regeneration condition in vivo because it shares the same physiological function, structure, morphology, and metabolic process as endogenous bone tissue. As a disruptive regenerative medicine technology, it has wide application prospects in the fields of organ development, gene editing, disease modeling, and precision therapy. Herein, the development process and physiological basis of different cell‐based bone organoids are reviewed, the current status of the application of different materials, cells, and construction methods for building bone organoids is described, and the prospects and challenges for the development of bone organoids in future medical fields is discussed.

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Stem cells for organoids

Cited by 21 publications

References 140 publications

Neuropeptide Y‐Mediated Gut Microbiota Alterations Aggravate Postmenopausal Osteoporosis

Neuropeptide Y‐Mediated Gut Microbiota Alterations Aggravate Postmenopausal Osteoporosis

Emerging diagnostic and therapeutic technologies based on ultrasound-triggered biomaterials

A Review of Advanced Biomaterials and Cells for the Production of Bone Organoid

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