Stem cells for the replacement of inner ear neurons and hair cells

Martinez-Monedero, Rodrigo

doi:10.1387/ijdb.072372rm

Cited by 35 publications

(18 citation statements)

References 84 publications

(71 reference statements)

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“…13 The cochear modiolus is also a target structure for future cell replacement therapy of the inner ear. 14,15 To our knowledge, ours is theˆrst report evaluating enhancement of the cochlear modiolus by diagnostic imaging.…”

Section: Discussionmentioning

confidence: 92%

“…Because human sensory epithelium and spiral ganglion neurons do not regenerate after injury or aging, cell replacement is highly anticipated for treating hearing loss and other disorders of the inner ear. 14,15 Stem cells and embryonic neurons would be transplanted to the scala tympani through the round window membrane, the most accessible route from outside the inner ear. In an animal model, implanted cells were found near spiral ganglions after migrating through pores in the cochlear modiolus.…”

Section: Discussionmentioning

confidence: 99%

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MR Imaging of the Cochlear Modiolus after Intratympanic Administration of Gd-DTPA

et al. 2010

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Purpose: We evaluated whether enhancement of the cochlear modiolus could be visualized 24 hours after intratympanic injection of gadolinium diethylenetriamine penta-acetic acid (Gd-DTPA) using a 3-dimensional real inversion recovery sequence combined with a 32-channel head coil at 3 tesla. Intratympanic injection of Gd-DTPA has been reported for visualizing endolymphatic hydrops in Meniere's disease, and its use has shown communication between the cochlear perilymph and cerebrospinal ‰uid in the internal auditory canal. Although the cochlear modiolus has been considered the route for this communication, this has not been conˆrmed through direct visualization of its enhancement.Materials and Methods: We qualitatively and quantitatively evaluated the presence of contrast enhancement in the modiolus in 19 patients with clinically suspected endolymphatic hydrops or hearing loss who underwent imaging as described above.Results: The contrast ratio (CR) between the cochlear modiolus and cerebellar white matter on the injected side was 1.09±1.23, and that on the non-injected side was -0.48± 0.38 (Pº0.01). In all subjects, the CR value was larger on the injected than non-injected side, and enhancement of the cochlear modiolus was also recognized visually.Conclusions: Intratympanic Gd-DTPA can be administered to visualize enhancement of the cochlear modiolus and may thereby reveal its functional anatomy.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

MR Imaging of the Cochlear Modiolus after Intratympanic Administration of Gd-DTPA

et al. 2010

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“…Similar results were previously obtained by Diensthuber et al (2009) for cultures of cochlear epithelial cells of different mice lineages (BALB/c and Math-1/nuclear green fluorescence protein (nGFP) transgenic mice) using similar culture medium. Media containing DMEM-High glucose and DMEM-F12 (1:1) have been proven to be successful for culturing rodent cochlea cells and were suitable for the applied purposes (Chao et al 2013;White et al 2012;Diensthuber et al 2009;Yerukhimovich et al 2007;Savary et al 2007;Martinez-Monedero and Edge 2007;Doetzlhofer et al 2004;Li et al 2003). In our adherent cultures, cochlear epithelial cells have typical fibroblast-like morphology, as observed for other mesenchymal stem cell cultures, including multipotentiality (Favaron et al 2014;Chao et al 2013;Wenceslau et al 2012).…”

Section: Discussionmentioning

confidence: 76%

Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

et al. 2017

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Hearing loss caused by the damage of cochlea sensory cells or neurons is a common human disease, but also affects dogs and other animals. To test their progenitor nature as potential value for future therapies, we characterized cells derived from the cochlear epithelium in dog fetuses. In total, 8 fetuses of 35-40 days of gestation, derived from castration campaigns, were investigated. Cells were analysed by the MTT colorimetric assay and in regard to cell cycle, differentiation capacities, immunophenotypes and qPCR analysis. In culture, cells had a fibroblast-like morphology. Phenotypic immunocharacterization showed positive staining for mesenchymal stem cell and pluripotency markers and were negative for hematopoietic cell markers. Cells possessed differentiation capacity for the three main cell lineages: osteogenic, adipogenic and chondrogenic, altogether indicating their nature as mesenchymal stem cells. Thus, cells derived from fetal cochlear tissues indeed may provide valuable sources of progenitor cells for cell therapy of canine deafness and other diseases.

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“…Neural stem cell marker nestin is expressed in the developing and mature mammalian inner ear (4,6). Despite ethical and immunological concerns transplanted stem cells hold considerable interest for regenerating auditory neurons and even hair cells (7).…”

Section: Introductionmentioning

confidence: 99%

Differentiation of human neural progenitor cell-derived spiral ganglion-like neurons: a time-lapse video study

Edin¹,

Liu²,

Boström³

et al. 2014

Acta Oto-Laryngologica

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Differentiation of human neural progenitor cell-derived spiral ganglion-like neurons: a time-lapse video study.Acta 134 (5) Objective: To establish an in vitro model for the human auditory nerve to replace and complement in vivo animal experiments and ultimately human in vivo transplantation.Methods: Human neural progenitors were differentiated under conditions developed for in vitro survival of human primary spiral ganglion culture with media containing growth factors associated with inner ear development. Differentiation was documented using time-lapse video microscopy. Time-dependent marker expression was evaluated using immunocytochemistry with fluorescence-and laser confocal microscopy.Results: Within 14 days of differentiation, neural progenitors adopted neural phenotype and expressed spiral ganglion-associated markers.

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Stem cells for the replacement of inner ear neurons and hair cells

Cited by 35 publications

References 84 publications

MR Imaging of the Cochlear Modiolus after Intratympanic Administration of Gd-DTPA

MR Imaging of the Cochlear Modiolus after Intratympanic Administration of Gd-DTPA

Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

Differentiation of human neural progenitor cell-derived spiral ganglion-like neurons: a time-lapse video study

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