Stem cells from adipose tissue improve the time of wound healing in rats

Abstract

PURPOSE:To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS:Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS:All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions.CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.

Risk factors and complications after body‐contouring surgery and the amount of stromal vascular fraction cells found in subcutaneous tissue

“…Knowledge about the stromal vascular fraction (SVF) and thus, adipose‐derived stem cell (ASC) physiology is of paramount interest in regenerative medicine and plastic surgery since many potential uses for clinical application have been described. Mostly, translational research puts its focus and efforts on applications of multipotent ASCs that are widely known to be important in wound healing . These cells make up for about two‐thirds of the CD45‐negative cells within the SVF, which is thus representative for the amount of ASCs in the tissue, and there is no difference in cell yield between superficial and deep subcutaneous fat layers .…”

“…Mostly, translational research puts its focus and efforts on applications of multipotent ASCs that are widely known to be important in wound healing. 1,2 These cells make up for about two-thirds of the CD45-negative cells within the SVF, which is thus representative for the amount of ASCs in the tissue, and there is no difference in cell yield between superficial and deep subcutaneous fat layers. 3 Recent literature suggests that the therapeutic effect of ASCs is partially mediated by the secretion of cytokines, and thus they are able to stimulate local fibroblasts of the wound bed 4,5 to promote wound healing and angiogenesis.…”