Stem cells from amniotic fluid – Potential for regenerative medicine

Loukogeorgakis, Stavros; Coppi, Paolo De

doi:10.1016/j.bpobgyn.2015.08.009

Cited by 58 publications

(49 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…α‐SMA was also highly expressed, correlating with a study by Lee et al This marker is commonly used as a marker of myofibroblast formation and is also expressed by stromal fibroblastic cells, whilst it also upregulates fibroblast contractile activity . Many studies also report on the feasibility of AF‐MSCs in the differentiation toward myogenic fate by targeted differentiation . By demonstrating the expression of intracellular markers, we suggest the inclusion of this analysis when characterizing MSCs from different sources and species, in addition to characterization by surface markers.…”

Section: Discussionsupporting

confidence: 84%

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

View full text Add to dashboard Cite

Over the years, there has been much confusion in defining molecular markers of mesenchymal stem cells (MSCs) for other than human species due to a lack of species-specific antibodies. Therefore, the aim of our study was to define rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs) and to reflect upon the current identification of AF-MSCs by providing a summary of detected surface markers in different species. The expression of rAF-MSC surface markers was analyzed at the protein and mRNA level. Flow cytometry analyses showed that rAF-MSCs were positive for CD29 and CD44, low positive for CD90, but negative for CD73, CD105, and CD166. Interestingly, RT-PCR (reverse transcription-polymerase chain reaction) exposed a discprepancy between transcribed mRNA and protein expression, as the cells expressed mRNA of all MSC markers: CD29, CD44, CD73, CD90, CD105, and CD166. Our results also confirmed the mesenchymal nature of isolated cells by morphology, ultrastructure, and intracellular marker expression profile. In addition, the expression of few pluripotency markers was also detected. We also found that passaging did not affect apoptosis and viability. Similarly, changes in karyotype were not observed during passaging. In conclusion, the provided characteristics may be used as a comprehensive set of criteria to define and characterize rAF-MSCs, required for the identification of these cells in preclinical investigations. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1601-1613, 2017.

show abstract

Section: Discussionsupporting

confidence: 84%

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…Despite presenting an intermediate phenotype between adult mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs), SS- and RS-hAFSCs are routinely cultivated in conditions that support the adhesion and expansion of multipotent somatic fetal and adult stem cells (MT culture conditions), i.e. on uncoated plastic dishes either in Chang medium, DMEM or α-MEM supplemented with various concentration of FBS 3 . Whilst hAFSCs proliferate and maintain a stable phenotype in MT culture conditions, little is known about the optimal requirement of these cells to maintain their original in vivo phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Human amniotic stem cells (hAFSCs) hold great therapeutic potential in regenerative medicine because they can be isolated from mid-trimester and term amniotic fluid samples without ethical restrictions, and are developmentally more primitive than adult stem cells 1–3 .…”

Section: Introductionmentioning

confidence: 99%

“…either in Dulbecco’s Modified Eagle’s Medium (DMEM), α-minimum essential medium (α-MEM) or Chang medium B and C supplemented with 5–15% fetal bovine serum (FBS), designed to sustain the phenotype of adult mesenchymal stem cells (MSCs) 3, 5, 7 . However, hAFSCs have been described as having a privileged intermediate phenotype between embryonic stem cells (ESCs) and adult MSCs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production

Hau

Ranzoni

Vlahova

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.

show abstract

“…Recently, researchers have identified the amniotic fluid as an untapped source of stem cells that are multipotent, possess immunomodulatory properties and do not have the ethical and legal limitations of embryonic stem cells (Park et al, 2011;Loukogeorgakis and De Coppi, 2016). Due to their ease of isolation, high proliferation rate, pluripotency features, low immunogenicity and inability to form tumors, AFMSCs are a promising type of stem cell for allogeneic cell transplantations in diseases of mesenchymal origin (Phermathai et al, 2010;Colosimo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Kováč¹,

Vašíček²,

Kulíková³

et al. 2017

JCEA

View full text Add to dashboard Cite

The isolation of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) has been already shown in human and several other species including rabbit. However, prior to the preclinical research on various animal models it is desirable to define AF-MSCs by a panel of surface protein markers. Therefore, the aim of this preliminary study was to detect the expression of several protein markers on the surface of AF-MSCs isolated from local breed of Nitra rabbit. Amniotic fluid was collected from humanely sacrificed rabbits (n = 3) and AF-MSCs were cultured to a third passage. Flow cytometry was used to detect surface protein marker expression and for viability testing. Rabbit AF-MSCs (rAF-MSCs) were also analyzed by transmission electron microscopy to define the ultrastructure. rAF-MSCs showed both sufficient viability (more than 80%) and low apoptosis rates at third passage and highly expressed CD29 (88.17 ± 7.17%) and CD44 (80.00 ± 2.28%). However, a dim expression of CD90 (17.24 ± 1.31%) and negative expression of CD73 (1.21 ± 0.56% and 4.41 ± 1.46%), CD105 (1.67 ± 0.37%) and CD166 (0.96 ± 2.26%) was observed. Additionally, ultrastructure analysis revealed eccentrically located nucleoli, an abundance of thin pseudopodia on cells' surfaces and proved the presence of typical mesenchymal stem cell features. In conclusion, this set of data contributes to more detailed information on rAF-MSCs, which were previously proposed feasible for preclinical stem cell research and as a suitable source for the cryopreservation of animal genetic resources in gene bank.

show abstract

Stem cells from amniotic fluid – Potential for regenerative medicine

Cited by 58 publications

References 66 publications

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production

Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit

Contact Info

Product

Resources

About