Stem Cells Giving Rise to Extraembryonic Tissues

Григорьева, Э. В.; Shevchenko, Alexander I.; Zhelezova, A. I.; Shilov, A. A.; Mazurok, Nina A.; Dyban, P. A.; Dyban, A. P.; Zakian, Suren M.

doi:10.1007/s10517-011-1179-1

Cited by 2 publications

(1 citation statement)

References 77 publications

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“…Previously, we have undertaken numerous attempts to obtain PSCs of common vole species of the genus Microtus from early pre-implantation blastocysts and germinal cells [ 30 ]. Multipotent cell lines of the pre-implantation embryo, such as trophoblast stem cells and extraembryonic endoderm cells, have been derived [ 25 , 31 - 33 ]. However, PSCs of common voles have not been obtained yet.…”

Section: Introductionmentioning

confidence: 99%

Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal

et al. 2015

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Every year, the list of mammalian species for which cultures of pluripotent stem cells (PSCs) are generated increases. PSCs are a unique tool for extending the limits of experimental studies and modeling different biological processes. In this work, induced pluripotent stem cells (iPSCs) from the hybrids of common voles Microtus levis and Microtus arvalis, which are used as model objects to study genome organization on the molecular-genetic level and the mechanisms of X-chromosome inactivation, have been generated. Vole iPSCs were isolated and cultured in a medium containing cytokine LIF, basic fibroblast growth factor (bFGF), ascorbic acid, and fetal bovine serum. Undifferentiated state of vole iPSCs is maintained by activation of their endogenous pluripotency genes – Nanog, Oct4, Sox2, Sall4, and Esrrb. The cells were able to maintain undifferentiated state for at least 28 passages without change in their morphology and give rise to three germ layers (ectoderm, mesoderm and endoderm) upon differentiation.

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Section: Introductionmentioning

confidence: 99%

Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal

et al. 2015

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Optimization of teratoma formation assay

Kizilova

2016

Genes & Cells

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Teratoma formation assay is necessary to estimate in vivo pluripotency of stem cells especially stem cell lines of human origin. Nevertheless convenient, valid and universal “standards” to analyze stem cell derived tumors have not been developed yet. New protocol for monitoring teratoma growth, morphological and histological analyzes of tumor samples is proposed in this paper. This protocol is oriented on review of tumors morphology and histology per se. The list-describer includes 17 obligate and 12 facultative diagnostic sell types and 7 diagnostic cell complexes. The protocol takes into account complicity and heterogeneity of teratoma structure and allows detect different morphological features of malignization process inside stem cell derived tumors in situ. The protocol was successfully applied for teratoma formation test which has been performed for 52 stem cell lines of different species origin (mouse, rat, аmerican mink and human). 326 stem cell derived tumors were completely described, reviewed and analyzed.

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Stem Cells Giving Rise to Extraembryonic Tissues

Cited by 2 publications

References 77 publications

Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal

Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal

Optimization of teratoma formation assay

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