Stem Cells in Fetal Tissue (The Kidney as a Model)

Pleniceanu, Oren; Dekel, Benjamin

doi:10.1007/978-1-4471-4171-6_9

Cited by 1 publication

(1 citation statement)

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“…However, to date, the knowledge about development of renal parenchyma is rather rudimentary. While numerous publications exist dealing with the formation of the kidney anlage and the induction of individual nephrons, sound information about successive development such as tube formation, polarization, spatial growth and physiological maturation of individual segments is limited [8]. The situation is further complicated as renal parenchyma does not derive from a single stem/progenitor cell lineage, but originates by interactions between nephrogenic mesenchymal and epithelial stem/progenitor cells.…”

Section: Introductionmentioning

confidence: 99%

The Interstitial Interface within the Renal Stem/Progenitor Cell Niche Exhibits an Unique Microheterogeneous Composition

Minuth

Denk²

2013

IJMS

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Repair of parenchyma by stem/progenitor cells is seen as a possible alternative to cure acute and chronic renal failure in future. To learn about this therapeutic purpose, the formation of nephrons during organ growth is under focus of present research. This process is triggered by numerous morphogenetic interactions between epithelial and mesenchymal cells within the renal stem/progenitor cell niche. Recent data demonstrate that an astonishingly wide interstitial interface separates both types of stem/progenitor cells probably controlling coordinated cell-to-cell communication. Since conventional fixation by glutaraldehyde (GA) does not declare in transmission electron microscopy the spatial separation, improved contrasting procedures were applied. As a consequence, the embryonic cortex of neonatal rabbit kidneys was fixed in solutions containing glutaraldehyde in combination with cupromeronic blue, ruthenium red or tannic acid. To obtain a comparable view to the renal stem/progenitor cell niche, the specimens had to be orientated along the cortico-medullary axis of lining collecting ducts. Analysis of tissue samples fixed with GA, in combination with cupromeronic blue, demonstrates demasked extracellular matrix. Numerous braces of proteoglycans cover, as well, the basal lamina of epithelial stem/progenitor cells as projections of mesenchymal stem/progenitor cells crossing the interstitial interface. Fixation with GA containing ruthenium red or tannic acid illustrates strands of extracellular matrix that originate from the basal lamina of epithelial stem/progenitor cells and line through the interstitial interface. Thus, for the first time, improved contrasting techniques make it possible to analyze in detail a microheterogeneous composition of the interstitial interface within the renal stem/progenitor cell niche.

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Section: Introductionmentioning

confidence: 99%