Stem Cells Loaded with Nanoparticles as a Drug Carrier for In Vivo Breast Cancer Therapy

Cao, Binrui; Yang, Mingying; Zhu, Ye; Qu, Xuewei; Mao, Chuanbin

doi:10.1002/adma.201401550

Cited by 101 publications

(77 citation statements)

References 38 publications

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“…Migration of pre-osteoclasts (RAW264.7) to GCTB microenvironment was tested using the transwell system (24-well plates) with 8-mm pore filters (BD Biosciences) by following our previously reported protocols [56,57]. In brief, RAW264.7 cells (5 × 10 4 ) were placed in the top chamber of the transwell, and GCTB cells (5 × 10 4 ) cultured with Mg alloy extracts were placed in the bottom chamber of the transwell as the chemoattractant.…”

Section: Methodsmentioning

confidence: 99%

Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone

Wang

Zhu

et al. 2018

Acta Biomaterialia

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In clinics, giant cell tumors of bone (GCTB) are removed by surgery. However, the resultant defects in bone still contain aggressive and metastatic GCTB cells that can recruit osteoclasts to damage bone, leading to new GCTB tumor growth and bone damage after tumor surgery. Hence, it is of high demand in developing a material that can not only fill the bone defects as an implant but also inhibit GCTB in the defect area as a therapeutic agent. More importantly, the molecular and cellular mechanism by which such a material inhibits GCTB growth has never been explored. To solve these two problems, we prepared a new biomaterial, the Mg-Sr alloys that were first coated with calcium phosphate and then loaded with a tumor-inhibiting molecule (Zoledronic acid, ZA). Then, by using a variety of molecular and cellular biological assays, we studied how the ZA-loaded alloys induced the death of GCTB cells (derived from patients) and inhibited their growth at the molecular and cellular level. At the cellular level, our results showed that ZA-loaded Mg-Sr alloys not only induced apoptosis and oxidative stress of GCTB cells, and suppressed their induced pre-osteoclast recruitment, but also inhibited their migration. At the molecular level, our data showed that ZA released from the ZA-loaded Mg-Sr alloys could significantly activate the mitochondrial pathway and inhibit the NF-κB pathway in the GCTB cells. Both mechanisms collectively induced GCTB cell death and inhibited GCTB cell growth. This work showed how a biomaterial inhibit tumor growth at the molecular and cellular level, increasing our understanding in the fundamental principle of materials-induced cancer therapy. This work will be interesting to readers in the fields of metallic materials, inorganic materials, biomaterials and cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone

Wang

Zhu

et al. 2018

Acta Biomaterialia

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“…Hypoxic‐treated neural stem cells showed the overexpression of CXCR4 and could enhance the tumor migration ability 23. There were numerous studies utilized the homing ability of stem cells for tumor targeting 24, 25, 26, 27, 28. In addition, it has been reported that iron oxide nanoparticles with the released iron ions could increase the CXCR4 expression on the mesenchymal stem cell (MSC) surface and promote the migration ability 29.…”

Section: Introductionmentioning

confidence: 99%

A Light‐Triggered Mesenchymal Stem Cell Delivery System for Photoacoustic Imaging and Chemo‐Photothermal Therapy of Triple Negative Breast Cancer

Feng

Yang

et al. 2018

Advanced Science

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Targeted therapy is highly challenging and urgently needed for patients diagnosed with triple negative breast cancer (TNBC). Here, a synergistic treatment platform with plasmonic–magnetic hybrid nanoparticle (lipids, doxorubicin (DOX), gold nanorods, iron oxide nanocluster (LDGI))–loaded mesenchymal stem cells (MSCs) for photoacoustic imaging, targeted photothermal therapy, and chemotherapy for TNBC is developed. LDGI can be efficiently taken up into the stem cells with good biocompatibility to maintain the cellular functions. In addition, CXCR4 on the MSCs is upregulated by iron oxide nanoparticles in the LDGI. Importantly, the drug release and photothermal therapy can be simultaneously achieved upon light irradiation. The released drug can enter the cell nucleus and promote cell apoptosis. Interestingly, light irradiation can control the secretion of cellular microvehicles carrying LDGI for targeted treatment. A remarkable in vitro anticancer effect is observed in MDA‐MB‐231 with near‐infrared laser irradiation. In vivo studies show that the MSCs‐LDGI has the enhanced migration and penetration abilities in the tumor area via both intratumoral and intravenous injection approaches compared with LDGI. Subsequently, MSCs‐LDGI shows the best antitumor efficacy via chemo‐photothermal therapy compared to other treatment groups in the TNBC model of nude mice. Thus, MSCs‐LDGI multifunctional system represents greatly synergistic potential for cancer treatment.

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“…1, 2 To achieve the ability of drug carriers in controlling cancer cells, most of the previous studies have focused on developing cancer targeting technology. 3–5 In fact, it is also very important to avoid or decrease the side effect resulting from burst release of drug from drug carriers in designing drug delivery as the burst released drug can be absorbed within a short time in vivo and accumulated in adjacent tissues and metabolic organs ( i.e.…”

Section: Introductionmentioning

confidence: 99%

In situ protein-templated porous protein–hydroxylapatite nanocomposite microspheres for pH-dependent sustained anticancer drug release

Shuai

Yang

et al. 2017

J. Mater. Chem. B

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Silk sericin, a water-soluble glue-like protein, is extensively used as a biomaterial due to its biocompatibility, hydrophilicity, biodegradability, and adequate resource. In addition, hydroxyapatite-based drug carriers are functionally efficient for drug or gene delivery due to their biodegradability, biocompatibility and easy metabolism in vivo. Herein, for the first time, this study used sericin, from a wild silkworm called Antheraea pernyi (A. pernyi), as a template to nucleate hydroxylapatite (HAp) nano-needles and form porous sericin-HAp nanocomposite microspheres as an anticancer drug carrier. Specifically, A. pernyi sericin (AS) was incubated in 1.5× simulated body fluid to induce the formation of porous AS/HAp microspheres in situ. Doxorubicin (DOX) loading and release assays proved that the microspheres exhibited pH-dependent controlled and sustained release of DOX. In particular, the microspheres can selectively release DOX at a higher rate at the acidic conditions typical for tumor microenvironment than at the physiological conditions typical for normal tissues, which will potentially reduce the side effect of the cancer drugs in normal tissues. Cancer cell toxicity assay, cancer cell imaging and intracellular DOX distribution assay provided further evidence to support the pH-dependent controlled and sustained release of DOX to cancer cells from the microspheres. Our work has demonstrated a biomimetic strategy for the design and synthesis of silk protein-based drug carriers that can be potentially employed in drug delivery and regenerative medicine.

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Stem Cells Loaded with Nanoparticles as a Drug Carrier for In Vivo Breast Cancer Therapy

Cited by 101 publications

References 38 publications

Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone

Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone

A Light‐Triggered Mesenchymal Stem Cell Delivery System for Photoacoustic Imaging and Chemo‐Photothermal Therapy of Triple Negative Breast Cancer

In situ protein-templated porous protein–hydroxylapatite nanocomposite microspheres for pH-dependent sustained anticancer drug release

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