Stem cells of aquatic invertebrates as an advanced tool for assessing ecotoxicological impacts

Rosner, Amalia; Armengaud, Jean; Ballarin, Loriano; Barnay‐Verdier, Stéphanie; Cima, Francesca; Coelho, Ana Varela; Domart‐Coulon, Isabelle; Drobne, Damjana; Genevière, Anne-Marie; Kokalj, Anita Jemec; Kotlarska, Ewa; Lyons, Daniel Mark; Mass, Tali; Paz, Guy; Pazdro, Ksenia; Perić, Lorena; Ramšak, Andreja; Rakers, Sebastian; Rinkevich, Baruch; Sugni, Michela; Cambier, Sébastien

doi:10.1016/j.scitotenv.2020.144565

Cited by 27 publications

(24 citation statements)

References 371 publications

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“…A. armata exudate caused an induction in the activity of the neurotoxicity biomarker AChE, mainly in an intermediate concentration. Hormesis response after exposure to several contaminants has been observed for AChE activity, mainly in the freshwater crustacean Daphnia magna, e.g., [73], but also verified in in vitro testing with cell cultures [74]. This response may reveal the activation of a compensatory mechanism allowing for plasticity in response to moderate adverse environments [75].…”

Section: Discussionmentioning

confidence: 69%

How Does Mytilus galloprovincialis Respond When Exposed to the Gametophyte Phase of the Invasive Red Macroalga Asparagopsis armata Exudate?

Coelho

Oliveira

Pires

et al. 2021

Water

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Asparagopsis armata is classified as an invasive species in Europe. Through the exudation of secondary metabolites, this macroalga holds a chemical defence against consumers, with potential toxic effects to native rocky shore communities. This study aims to evaluate the potential impact of A. armata (gametophyte) exudate in a native species, the mussel Mytilus galloprovincialis, in terms of biochemical and organismal effects. The 96 h-LC50 was 3.667% and based on it, exudate concentrations (0.25; 0.5; 1; 2%) were determined to further sublethal experiments. These sublethal concentrations caused no oxidative damage in the digestive gland since lipid peroxidation and protein carbonylation were not affected. Nevertheless, there was a significant rise in the electron transport system activity and total glutathione content in muscle, suggesting an increased non-enzymatic antioxidant capacity and consequent energy consumption to cope with potential pro-oxidant compounds. This might have contributed to the observed decline in cellular energy allocation of the exposed mussels. At the organismal level, clearance capacity declined along the concentration gradient. Moreover, the number of functional byssuses decreased with increasing concentrations and a significant reduction in their attachment strength was observed. These findings suggest that the presence of A. armata may compromise M. galloprovincialis integrity in the invaded coastal areas.

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Section: Discussionmentioning

confidence: 69%

How Does Mytilus galloprovincialis Respond When Exposed to the Gametophyte Phase of the Invasive Red Macroalga Asparagopsis armata Exudate?

Coelho

Oliveira

Pires

et al. 2021

Water

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“…Immune and stem cells of invertebrate species have already been recognized as valuable models in ecotoxicology for both in vitro and in vivo studies. However, rarely the behavior of both cell type and their cross-talk in preserving and restoring homeostasis have been used as ecotoxicological endpoints ( 7 , 11 ).…”

Section: Links Between Stem Cells and Immunitymentioning

confidence: 99%

“…Recently, the potential application of aquatic invertebrate ASCs in ecotoxicology has been underlined in a very comprehensive review ( 7 ). The authors point out that, due to their lower genetic complexity, aquatic invertebrate ASCs represent a valuable and reliable tool for understanding fundamental biological processes, mode of action of pollutants and mechanisms of epigenetic toxicity.…”

Section: Links Between Stem Cells and Immunitymentioning

confidence: 99%

“…The assessment of myelotoxicity provides a broad measure of the potential impacts of chemicals on the growth and development of immune cells, where all immune-related cells develop from pluripotent, hematopoietic stem cells residing in the bone marrow. This is not the situation in aquatic invertebrates, where stem cell-based bioassays have not yet been adequately explored as ecotoxicological tools ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

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Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

et al. 2021

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The scopes related to the interplay between stem cells and the immune system are broad and range from the basic understanding of organism’s physiology and ecology to translational studies, further contributing to (eco)toxicology, biotechnology, and medicine as well as regulatory and ethical aspects. Stem cells originate immune cells through hematopoiesis, and the interplay between the two cell types is required in processes like regeneration. In addition, stem and immune cell anomalies directly affect the organism’s functions, its ability to cope with environmental changes and, indirectly, its role in ecosystem services. However, stem cells and immune cells continue to be considered parts of two branches of biological research with few interconnections between them. This review aims to bridge these two seemingly disparate disciplines towards much more integrative and transformative approaches with examples deriving mainly from aquatic invertebrates. We discuss the current understanding of cross-disciplinary collaborative and emerging issues, raising novel hypotheses and comments. We also discuss the problems and perspectives of the two disciplines and how to integrate their conceptual frameworks to address basic equations in biology in a new, innovative way.

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“…Often, quantifying the concentrations of xenobiotics present in water is costly and does not provide the real impact on the organisms that live in the environment (Schirmer et al, 2011). Thus, ecotoxicological tests with biomarkers have been used to assess the effects of toxic chemicals on different ecosystems (Adams & Greeley, 2000;Rosner et al, 2021). In addition, their responses can be used to estimate exposure to chemicals or resulting effects on aquatic organisms (Ramsdorf et al, 2012;Araujo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic response of Macrobrachium jelskii (Miers, 1877) exposed to water from urban and rural rivers in Bahia, Brazil

Mota

Winkaler

Oliveira

et al. 2021

RSD

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Several biomarker enzymes such as catalase (CAT) and glutathione S-transferase (GST) can be used to measure oxidative stress in animals caused by exposure to xenobiotics. The objective of the present study was to characterize different points of the Capivari (CP1 and CP2), Paraguaçu (PG1 and PG2) and Subaé (SB1 and SB2) Rivers, state of Bahia, in relation to the presence of xenobiotics, using CAT and GST as bioindicators in M. jelskii. The water-sampling sites were considered urban or rural and in all of them signs of environmental degradation were observed. Therefore, acute exposure tests (96h) were performed with water samples collected during the dry and rainy seasons. Results showed that the activity of CAT and GST in prawns exposed to water from CP1 and CP2 were very similar, while those exposed to water from PG1, PG2, SB1 and SB2 formed distinct groups of data. Significant increase in the activity of at least one of the analyzed enzymes in each sampling site was observed, when compared to animals in the control group. This demonstrated a possible oxidative stress in M. jelskii caused by the presence of xenobiotics in the water (e.g., domestic sewage, pesticides, oil, and heavy metals). Enzymatic activities were higher in animals from experiments carried out in the rainy season, except for the CAT activity of animals exposed to water from Subaé River. This study demonstrated the potential of M. jelskii as bioindicator and contributed to the knowledge of aspects of the antioxidant defense system of this species.

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Stem cells of aquatic invertebrates as an advanced tool for assessing ecotoxicological impacts

Cited by 27 publications

References 371 publications

How Does Mytilus galloprovincialis Respond When Exposed to the Gametophyte Phase of the Invasive Red Macroalga Asparagopsis armata Exudate?

How Does Mytilus galloprovincialis Respond When Exposed to the Gametophyte Phase of the Invasive Red Macroalga Asparagopsis armata Exudate?

Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

Enzymatic response of Macrobrachium jelskii (Miers, 1877) exposed to water from urban and rural rivers in Bahia, Brazil

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