Stem Cells

Bongso, Ariff; Lee, Eng Hin

doi:10.1142/5729

Cited by 25 publications

(7 citation statements)

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“…Mesenchymal stem cells can be used for cell therapy because they are easy to isolate, can be expanded widely without losing their differentiation ability, have low immunity, and are multipotent. The main role of mature stem cells is maintaining and repairing the tissue in which they are located [ 36 ]. Mesenchymal stem cells provide benefits through two mechanisms: (1) Mesenchymal stem cells differentiate to replace damaged cells to form new cytoarchitecture and (2) mesenchymal stem cells produce paracrine effects by releasing immunoregulators that support regeneration (tropic activity).…”

Section: Discussionmentioning

confidence: 99%

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

et al. 2021

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Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models. Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences. Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased. Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis.

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Section: Discussionmentioning

confidence: 99%

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

et al. 2021

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“…Human embryos for hESC derivation are typically donated at the cleavage stage [ 15 , 16 , 17 ], frequently 5–10 years after cryopreservation [ 10 , 22 ] or after the identification of poor morphology [ 23 , 24 ]. These embryos are often of reduced quality [ 23 , 24 , 25 , 26 ], and give rise to ESC lines with low efficiency as a result [ 6 , 7 ]. The aim of the present study was to examine the effect of insulin treatment on blastocyst development, outgrowth formation, ESC progenitor cell number and the derivation of primary ESC colonies from cleavage stage embryos using a mouse model, as a first step towards improving hESC isolation efficiencies from embryos which have a reduced quality.…”

Section: Discussionmentioning

confidence: 99%

“…As such human ESCs (hESCs) have potential applications in regenerative medicine. However, the efficiency with which hESCs can be derived is generally lower [ 6 , 7 ] than that reported for mice [ 8 , 9 ]. One key difference between mESCs and hESCs, is that mouse embryos for ESC derivation are usually cultured in vivo , while many human embryos used for ESC research are frozen assisted reproduction technology (ART) embryos donated often up to 5–10 years after they were produced [ 10 ] due to regulatory requirements.…”

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confidence: 99%

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Epiblast Cell Number and Primary Embryonic Stem Cell Colony Generation Are Increased by Culture of Cleavage Stage Embryos in Insulin

Campbell

Lane

Vassiliev

et al. 2013

Journal of Reproduction and Development

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Human embryos for hESC derivation are often donated at the cleavage stage and of reduced quality. Poor quality embryos have lower efficiency for hESC derivation. However, cleavage stage mouse embryos develop into higher quality expanded blastocysts if they are cultured with insulin, suggesting that this approach could be used to improve hESC derivation from poor quality cleavage stage embryos. The present study used a mouse model to examine this approach. In particular we examined the effect of insulin on the number of epiblast cells in blastocysts on days 4, 5 and 6 using Oct4 and Nanog co-expression. Second we examined the effect of insulin on the frequency with which outgrowths can be derived from these. Finally, we tested whether prior culture in the presence of insulin results in blastocysts with increased capacity to generate ESC colonies. Culture of cleavage stage embryos with insulin increased the number of Oct4 and Nanog positive cells in blastocysts at all time points examined. Prior culture with insulin had no effect on outgrowths generated from blastocysts plated on days 4 or 5. However, insulin treatment of blastocysts plated on day 6 resulted in increased numbers of outgrowths with larger epiblasts compared with controls. 13% of insulin treated day 6 blastocysts produced primary ESC colonies compared with 6% of controls. In conclusion, treatment with insulin can improve epiblast cell number in mice leading to an increase with which primary ESC colonies can be generated and may improve hESC isolation from reduced quality embryos donated at the cleavage stage.

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“…Several approaches have been applied to address these challenges including functionalization of the scaffolds with bioactive molecules [17,18], combination of various types of biomaterials [19,20], incorporation of chemical ions and molecules [21,22] and nanomaterials [23][24][25][26][27], or a combination of these [28,29]. 3D multifunctional scaffolds are increasingly favored because they can mimic the structure of the natural ECM and provide a condition for the spatial organization of cells and enhancing the cell-cell interactions [7,8,13,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

The fate of mesenchymal stem cells is greatly influenced by the surface chemistry of silica nanoparticles in 3D hydrogel-based culture systems

Darouie

Majd

Rahimi

et al. 2020

Materials Science and Engineering: C

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Stem Cells

Cited by 25 publications

References 0 publications

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

Epiblast Cell Number and Primary Embryonic Stem Cell Colony Generation Are Increased by Culture of Cleavage Stage Embryos in Insulin

The fate of mesenchymal stem cells is greatly influenced by the surface chemistry of silica nanoparticles in 3D hydrogel-based culture systems

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