stem fasciated, a Recessive Mutation in Sunflower (Helianthus annuus), Alters Plant Morphology and Auxin Level

Fambrini, Marco; Bonsignori, Elisa; Rapparini, Francesca; Cionini, G.; Michelotti, V.; Bertini, D.; Baraldi, Rita; Pugliesi, Claudio

doi:10.1093/aob/mcl153

Cited by 32 publications

(33 citation statements)

References 96 publications

(119 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ray flowers were fixed as previously described (Fambrini et al 2006). Paraffin-embedded tissues were sectioned using a rotary microtome (Reichert, Vienna, Austria).…”

Section: Histological Analysismentioning

confidence: 99%

A transposon-mediate inactivation of a CYCLOIDEA-like gene originates polysymmetric and androgynous ray flowers in Helianthus annuus

2011

Self Cite

View full text Add to dashboard Cite

In several eudicots, including members of the Asteraceae family, the CYCLOIDEA (CYC) genes, which belong to the TCP class of transcription factors, are key players for floral symmetry. The sunflower inflorescence is heterogamous (radiate capitulum) with sterile monosymmetric ray flowers located in the outermost whorl of the inflorescence and hermaphrodite polysymmetric disk flowers. In inflorescence of Heliantheae tribe, flower primordia development initiates from the marginal ray flowers while disk flowers develop later in an acropetal fashion in organized parastichies along a number found to be one of Fibonacci patterns. Mutants for inflorescence morphology can provide information on the role of CYC-like genes in radiate capitulum evolution. The tubular ray flower (turf) mutant of sunflower shows hermaphrodite ray flowers with a nearly polysymmetric tubular-like corolla. Here, we demonstrate that this mutation is caused by the insertion in the TCP motif of a sunflower CYC-like gene (HaCYC2c) of non-autonomous transposable element (TE), belonging to the CACTA superfamily of transposons. We named this element Transposable element of turf1 (Tetu1). The Tetu1 insertion changes the reading frame of turf-HaCYC2c for the encoded protein and leads to a premature stop codon. Although in Tetu1 a transposase gene is lacking, our results clearly suggest that it is an active TE. The excision of Tetu1 restores the wild type phenotype or generates stable mutants. Co-segregation and sequence analysis in progenies of F(2) and self-fertilized plants derived from reversion of turf to wild type clearly identify HaCYC2c as a key regulator of ray flowers symmetry. Also, HaCYC2c loss-of-function promotes the developmental switch from sterile to hermaphrodite flowers, revealing a novel and unexpected role for a CYC-like gene in the repression of female organs.

show abstract

“…Ray flowers were fixed as previously described (Fambrini et al 2006). Paraffin-embedded tissues were sectioned using a rotary microtome (Reichert, Vienna, Austria).…”

Section: Histological Analysismentioning

confidence: 99%

A transposon-mediate inactivation of a CYCLOIDEA-like gene originates polysymmetric and androgynous ray flowers in Helianthus annuus

2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…The plant growth conditions used to cultivate the inbred line F11 and the WC mutant were reported by Fambrini et al [60]. Briefly, plants were grown in the field in replicate rows (50 cm interrow spacing with 25 -30 cm between plants; ca.…”

Section: Experimental Partmentioning

confidence: 99%

Pollen Aroma Fingerprint of two Sunflower (Helianthus annuus L.) Genotypes Characterized by Different Pollen Colors

Bertoli

Fambrini

Doveri

et al. 2011

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

Samples of fresh pollen grains, collected from capitula in full bloom from two genotypes of sunflower (Helianthus annuus L.) and characterized by a different color, i.e., white-cream (WC) and orange (O), were analyzed by the HS-SPME (headspacesolid phase microextraction)/GC/MS technique. This study defined for the first time the fingerprint of the sunflower pollen, separated from the disc flowers, to define its contribution to the inflorescence aroma. In the GC/MS fingerprints of the WC and O genotypes, 61 and 62 volatile compounds were identified, respectively. Monoterpene hydrocarbons (34% in O vs. 28% in WC) and sesquiterpene hydrocarbons (37% in O vs. 31% in WC) were ubiquitous in all samples analyzed and represented the main chemical classes. α-Pinene (21% in O vs. 20% in WC) and sabinene (11% in O vs. 6% in WC) were the dominant volatiles, but also a full range of aliphatic hydrocarbons and their oxygenated derivatives gave a decisive contribution to the aroma composition (10% in O vs. 12% in WC). In addition, dendrolasin (3% in O vs. 4% in WC) and some minor constituents such as (E)-hex-2-en-1-ol (0.4% in O vs. 0.1% in WC) were pointed out not only for their contribution to the pollen scent, but also for their well-known role in the plant ecological relationships. Having evaluated two pollen morphs with different carotenoid-based colors, the study sought to highlight also the presence of some volatile precursors or derivatives of these pigments in the aroma. However, the pollen aroma of the two selected genotypes made a specific chemical contribution to the sunflower inflorescence scent without any influence on carotenoid derivatives.

show abstract

“…Changes in hormone metabolism also correlated with fasciation in hybrid Populus transformed with the Agrobacterium rhizogenes rolC gene, and fasciated tissues had a high level of free cytokinins (Nilsson et al 1996). It has also been shown that plants of Helianthus annuus with fasciated stems (STF) had higher endogenous free IAA levels (Fambrini et al 2006). The observed phenotype and the higher levels of auxin detected suggest that the STF gene is necessary for the proper initiation of primordia and for the establishment of a phyllotactic pattern through control of both shoot apical meristem arrangement and hormonal homeostasis (Fambrini et al 2006).…”

Section: Hormonal Content In Symptomless Versus Fasciated Tissuesmentioning

confidence: 94%

“…It has also been shown that plants of Helianthus annuus with fasciated stems (STF) had higher endogenous free IAA levels (Fambrini et al 2006). The observed phenotype and the higher levels of auxin detected suggest that the STF gene is necessary for the proper initiation of primordia and for the establishment of a phyllotactic pattern through control of both shoot apical meristem arrangement and hormonal homeostasis (Fambrini et al 2006). Furthermore, the increased hormonal levels point to a possible hormonal control mechanism of the development of fasciated shoot apical meristem phenotypes.…”

Section: Hormonal Content In Symptomless Versus Fasciated Tissuesmentioning

confidence: 99%

Molecular identification of phytoplasmas in fasciated cacti and succulent species and associated hormonal perturbation

Omar

Dewir

El-Mahrouk

2014

Journal of Plant Interactions

View full text Add to dashboard Cite

Fasciation, a frequent phenomenon in Cactaceae, has been attributed to various causes. The present study reports on phytoplasma-induced fasciation in Euphorbia coerulescens (Euphorbiaceae), Orbea gigantea (Asclepiadaceae), Opuntia cylindrica (Cactaceae), and Senecio stapeliiformis (Asteraceae). DNA was extracted from symptomless and fasciated tissues and amplified by nested PCR using universal primers P1/P7 followed by R16F2n/R16R2 produced amplicons of 1.2 Kb. The nucleotide sequence analyses of the amplicons indicated that fasciated plants were infected by phytoplasma. Phylogenetic analysis placed the cacti fasciation phytoplasmas in 16SrII group. The hormonal content of symptomless and fasciated tissues including indole-3-acetic acid (IAA), kinetin (Kin), N 6 -benzyladenine (BA), abscisic acid (ABA), and gibberellic acid (GA 3 ) was quantified by high performance liquid chromatography (HPLC). The results indicated that fasciation in O. gigantea was correlated with the accumulation of Kin and IAA increasing five and two times, respectively, as compared to symptomless tissue. However, there was no consistent pattern of hormones in other fasciated species (E. coerulescens, O. cylindrica, and S. stapeliiformis), suggesting that different plant species might have different mechanism to develop fasciation associated with phytoplasma infection.

show abstract

stem fasciated, a Recessive Mutation in Sunflower (Helianthus annuus), Alters Plant Morphology and Auxin Level

Abstract: The observed phenotype and the high level of auxin detected in stf plants suggest that the STF gene is necessary for the proper initiation of primordia and for the establishment of a phyllotactic pattern through control of both SAM arrangement and hormonal homeostasis.

Cited by 32 publications

References 96 publications

A transposon-mediate inactivation of a CYCLOIDEA-like gene originates polysymmetric and androgynous ray flowers in Helianthus annuus

A transposon-mediate inactivation of a CYCLOIDEA-like gene originates polysymmetric and androgynous ray flowers in Helianthus annuus

Pollen Aroma Fingerprint of two Sunflower (Helianthus annuus L.) Genotypes Characterized by Different Pollen Colors

Molecular identification of phytoplasmas in fasciated cacti and succulent species and associated hormonal perturbation

Contact Info

Product

Resources

About