Stem loop-mediated isothermal amplification test: comparative analysis with classical LAMP and PCR in detection of Entamoeba histolytica in Kenya

Mwendwa, Fridah; Mbae, Cecilia; Kinyua, Johnson; Mulinge, Erastus; Mburugu, Gitonga Nkanata; Njiru, Z. K.

doi:10.1186/s13104-017-2466-3

Cited by 16 publications

(13 citation statements)

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“…More recently, several research groups have reported the use of other dyes for end-point reads of LAMP assays, with potential applications for ZIKV. These include malachite green dye [72,73], hydroxynaphthol blue dye [74,75], evagreen dye [76,77], Goldview II dye [78], Gelred dye [79,80], SYTO fluorescent dye [81,82], and berberine dye [83].…”

Section: Step 3: Lamp Modes Of Outputmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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Section: Step 3: Lamp Modes Of Outputmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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“…The end products of LAMP gene amplification will be stem-loop DNA structures with several inverted repeats of the target and cauliflower-like structures with multiple loops which cause the gel electrophoresis image of these products to be in ladder-like patterns. This method has been used for detection of E. histolytica (Mwendwa et al 2017), S. aureus (Chen et al 2017a), V. parahaemolyticus (Siddique et al 2017), Clostridium perfringens (Radhika et al 2016), parasitic tapeworm Echinococcus spp. , and plant disease caused by Southern tomato virus (ElviraGonzalez et al 2017).…”

Section: Lamp End-point Detection Methodsmentioning

confidence: 99%

“…Other dyes such as malachite green dye (Sriworarat et al 2015;Lucchi et al 2016;Nzelu et al 2016), Picogreen dye (Batra et al 2015), Gelred dye (Wozniakowski et al 2013;Wassermann et al 2014), SYTO fluorescent dye (Liu et al 2013;Watts et al 2014;Mwendwa et al 2017) Zhou et al, 2014). The reaction between free calcein and magnesium ions intensified the fluorescence of LAMP product (ii.)…”

Section: Lamp End-point Detection Methodsmentioning

confidence: 99%

“…[Colour figure can be viewed at wileyonlinelibrary.com] instance, Salmonella typhi (Abdullah et al 2014;Fan et al 2015), Campylobacter jejuni and Campylobacter coli (Pham et al 2015), and Helicobacter pylori (Bakhtiari et al 2016;Yari et al 2016). Other successful examples of LAMP usage include Neisseria meningitidis (Lee et al 2015), Listeria monocytogenes (Birmpa et al 2015;Wang et al 2015b;Ye et al 2015), Entamoeba histolytica (Rivera and Ong 2013;Singh et al 2013;Mwendwa et al 2017), and human leptospirosis (Seesom et al 2015;Hsu et al 2017). LAMP was also efficiently formulated against several multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii (Kim et al 2016b).…”

Section: B3cmentioning

confidence: 99%

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Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

et al. 2018

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“…Широкое применение нашли интеркалирующие красители SYBR Green I [12,13], PicoGreen [14,15], Eva Green [16], Goldview II [17,18], SYTO [19,20], пропидиум йодид [21]. дешевым аналогом интеркалирующих красителей может являться алкалоид барберин, содержащийся в различных частях многих растений.…”

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Prospects For the Use of Loop Isothermal Amplification in the Diagnosis of Particularly Dangerous Infectious Diseases Caused by the Viruses of the Pathogenicity Group I

Карташов

Chub

Микрюкова

et al. 2020

Problemy Osobo Opasnykh Infektsii

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Dangerous viral infectious diseases pose a serious threat to human life and health, as their uncontrolled spread leads to the development of major outbreaks and epidemics. Rapid and accurate detection of the pathogen is an essential component of the fight against infectious diseases. This review is devoted to loop-mediated isothermal amplification (LAMP), which is one of the simplest and most reliable methods of molecular-genetic research that meets modern requirements. The simplicity of the analysis and registration of the obtained results, which is necessary under conditions with minimal laboratory capacities, makes it possible to consider this type of diagnostic technology as the most promising, which allows us to identify genetic markers (DNA or RNA) of pathogens of dangerous infectious diseases in the shortest possible time. Objective of the review is to summarize and systematize the data available to date on the use of LAMP for detecting RNA of dangerous infectious diseases caused by the Ebola,Marburg and Lassa viruses. The paper discusses the basic principles of the loop isothermal amplification reaction, the components that make up the reaction mixture and are used for the analysis, as well as methods for detecting the results obtained. When studying the information available in the literature sources about the advantages and disadvantages of LAMP, it is shown that in many cases, isothermal amplification is not inferior in sensitivity and specificity to the main molecular-genetic diagnostic methods currently used. Modifications that can be used for accelerated diagnostics of RNA-containing viruses are also considered.

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Stem loop-mediated isothermal amplification test: comparative analysis with classical LAMP and PCR in detection of Entamoeba histolytica in Kenya

Cited by 16 publications

References 40 publications

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

Prospects For the Use of Loop Isothermal Amplification in the Diagnosis of Particularly Dangerous Infectious Diseases Caused by the Viruses of the Pathogenicity Group I

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