Stenotrophomonas maltophilia healthcare-associated infections: identification of two main pathogenic genetic backgrounds

Corlouer, Camille; Lamy, Brigitte; Desroches, Marine; Ramos-Vivas, José; Mehiri-Zghal, E.; Lemenand, Olivier; Decousser, J.-W.; Aberanne, S.; Belmonte, Olivier; Blondiaux, Nicolas; Cattoir, Vincent; Dekeyser, S.; Delarbre, J.-M.; Jaouen, Anne-Christine; Laurens, E.; Duchêne, Éric; Pangon, B.; Plassart, Claire; Picot, Stéphane; Vachée, Anne

doi:10.1016/j.jhin.2017.02.003

Cited by 26 publications

(36 citation statements)

References 21 publications

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“…The genogroup organization is robust enough to be confirmed by different molecular methods, such as amplified fragment polymorphism (AFLP), gyrB restriction fragment length polymorphism (RFLP) analysis, and multilocus sequence typing (MLST) [3,4,7]. We recently applied this last method to a large sample of human strains responsible for health care-associated infections, and we underlined the high prevalence of genogroup 6 and, more surprisingly, genogroup 2 [8]. Unfortunately, the various previously published studies did not include any strains originating from animals, even though Sm is responsible for colonization and infection in animals, particularly in horses [1,4,5,7,9,10].…”

Section: Introductionmentioning

confidence: 99%

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Jayol¹,

Corlouer²,

Haenni³

et al. 2018

Eur J Clin Microbiol Infect Dis

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Stenotrophomonas maltophilia (Sm) is an archetypal environmental opportunistic bacterium responsible for health care-associated infections. The role of animals in human Sm infections is unknown. This study aims to reveal the genetic and phylogenetic relationships between pathogenic strains of Sm, both animal and human, and identify a putative role for animals as a reservoir in human infection. We phenotypically and genotypically characterized 61 Sm strains responsible for animal infections (mainly respiratory tract infections in horses) from a French nationwide veterinary laboratory network. We tested antimicrobial susceptibility and performed MLST and genogrouping using the concatenation of the seven housekeeping genes from the original MLST scheme. Excluding the eight untypeable strains owing to the lack of gene amplification, only 10 out of the 53 strains yielded a known ST (ST5, ST39, ST162, ST8, ST27, ST126, ST131). The genogroup distribution highlighted not only genogroups (genogroups 5 and 9) comprised exclusively of animal strains but also genogroups shared by human and animal strains. Interestingly, these shared genogroups were primarily groups 2 and 6, which have previously been identified as the two most frequent genogroups among human-pathogenic Sm strains, especially among respiratory pathogens. The antimicrobial susceptibility testing underlined the presence of acquired resistance: 18.8 and 7.5% of the tested isolates were resistant to the sulfonamide-trimethoprim combination and ciprofloxacin, respectively. Animal strains of Sm shared phylogenetic traits with some of the most successful human strains. The exact relationships between the human and animal strains, and the genetic support of these common traits, need to be determined.

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Section: Introductionmentioning

confidence: 99%

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Jayol¹,

Corlouer²,

Haenni³

et al. 2018

Eur J Clin Microbiol Infect Dis

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“…Antibiotic resistance is common in NF-GNB, resulting in inappropriate initial antibiotic treatment and poor outcomes 1,11,12,13 . Several outbreaks of infection have been described including a few reports of infections originating outside the hospital setting 14,15,16,17,18 .…”

Section: Discussionmentioning

confidence: 99%

Stenotrophomonas maltophilia isolates in a tertiary care hospital in Northeastern Brazil

Lemos

Paixão

Santos³

et al. 2019

BJHR

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Introduction: Stenotrophomonas maltophilia (S.maltophilia) has emerged as an important opportunistic, nosocomial pathogen capable of causing respiratory, bloodstream and urinary infections in a debilitated host. Prevention of acquisition and infection depends upon the application of modern infection-control practices. Methods: We conducted a retrospective and observational laboratory-based study on S. maltophilia to evaluate the distribution of these pathogen isolates between February 2008 and December 2012 in a 38-bed intensive care unit in northeastern Brazil. The study began by surveying all positive S. maltophilia cultures processed by the microbiology laboratory during the period of study. The cultures, originating from various types of biological material were processed by Vitek® System. The cultures were processed in protocol in accordance with CLSI-M100-S25. The incidence rate was calculated per 1,000 admissions. Results: A total of 100 sample isolates were tested positive for S. maltophilia. The isolates were obtained from 50.301 hospitalized patients (0.19%). A total of 8.572 positive cultures were detected in different biological materials. Ninety four (94%) isolates were from patients in adult intensive care. Seventy (70%) S. maltophilia isolates were from tracheal cultures. All S. maltophilia isolates were sensitive to Co-trimoxazole and levofloxacin. Conclusions: This study shows the importance of other analytical epidemiologic studies that correlate the presence of S. maltophilia incidence associated with the risk factor, and the outcomes, in our region.

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“…Ülkemizde gerçekleştirilen çalışmalarda TMP-SXT direncini % 4.8 ile % 15.2 aralığında tespit etmişlerdir (9,10,14,17,21,22) . Yurtdışı çalışmalarda TMP-SXT direncini Meksika'da (11) % 25; Çin'de (12) % 38.7, Fransa'da (5) % 5 olarak belirtmişlerdir.…”

Section: Discussionunclassified

“…Yurtiçi çalışmalarda, seftazidim direnci % 77.6 ile % 78 aralığında saptanmıştır (9,14,21) . Seftazidim direncini yurtdışı çalışmalarda Fransa'da % 70, Kuzey Amerika'da % 34.9, Asya-Pasifik bölgesinde de % 53.5 olarak tespit etmişlerdir (5,7) . Çalıştığımız S.maltophilia suşlarında en yüksek direnç % 70 ile seftazidime karşı saptanmıştır.…”

Section: Discussionunclassified

“…Corlouer ve ark. (5) siprofloksasine karşı Fransa'da % 17 direnç saptarken levofloksasin direnç oranı yurtdışı çalışmalarda Tayvan'da (23) % 8; Meksika'da (11) % 2.6; Çin'de (12) % 19.6 tespit etmişlerdir. Asya Pasifik bölgesinde gerçekleştirilen Antimikrobiyal Direnç Eğilimleri İzleme Çalışması'nda intraabdominal enfeksiyonlarla ilişkili S.maltophilia izolatlarında % 26.5 direnç oranıyla levofloksasine direnç geliştiği gözükmektedir (15) .…”

Section: Discussionunclassified

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Antibiotic Susceptibility of Stenotrophomonas maltophilia Isolated from Various Clinical Specimens

Arabacı¹,

Yanılmaz²,

Uzun³

2019

Ankem Derg

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Tigesiklin minimum inhibitör konsantrasyon (MİK) değeri 2 mg/l'nin üzerinde dirençli kabul edildiğinde tigesikline % 83 duyarlılık saptanmıştır. Çalışmamızda, seftazidime karşı saptanan direnç nedeniyle, üçüncü kuşak sefalosporinlerin hastanemizde S.maltophilia'nın ampirik tedavisinde kullanılamayacağı sonucuna varılmıştır. TMP-SXT ve levofloksasin direncimiz henüz korkulacak boyutlarda olmamasına rağmen artan direnç oranları açısından dikkat edilmesi gerektiği görülmüştür.

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Stenotrophomonas maltophilia healthcare-associated infections: identification of two main pathogenic genetic backgrounds

Cited by 26 publications

References 21 publications

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Stenotrophomonas maltophilia isolates in a tertiary care hospital in Northeastern Brazil

Antibiotic Susceptibility of Stenotrophomonas maltophilia Isolated from Various Clinical Specimens

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