Stenotrophomonas maltophilia interferes via the DSF-mediated quorum sensing system with Candida albicans filamentation and its planktonic and biofilm modes of growth

Rossi, Beatriz N. Passerini de; García, Carlos; Alcaraz, Eliana; Franco, Mirta

doi:10.1016/s0325-7541(14)70084-7

Cited by 26 publications

(24 citation statements)

References 36 publications

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“…fumigatus or S . maltophilia and other microorganisms [15, 32–35], but never between these two microorganisms. The originality of this study was to develop for the first time an in vitro model of mixed biofilm of A .…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm

et al. 2016

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BackgroundBiofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S. maltophilia and A. fumigatus by using analytical and quantitative approaches.Materials and MethodsAn A. fumigatus strain (ATCC 13073) expressing a Green Fluorescent Protein (GFP) and an S. maltophilia strain (ATCC 13637) were used. Fungal and bacterial inocula (105 conidia/mL and 106 cells/mL, respectively) were simultaneously deposited to initiate the development of an in vitro mixed biofilm on polystyrene supports at 37°C for 24 h. The structure of the biofilm was analysed via qualitative microscopic techniques like scanning electron and transmission electron microscopy, and fluorescence microscopy, and by quantitative techniques including qPCR and crystal violet staining.ResultsAnalytic methods revealed typical structures of biofilm with production of an extracellular matrix (ECM) enclosing fungal hyphae and bacteria. Quantitative methods showed a decrease of A. fumigatus growth and ECM production in the mixed biofilm with antibiosis effect of the bacteria on the fungi seen as abortive hyphae, limited hyphal growth, fewer conidia, and thicker fungal cell walls.ConclusionFor the first time, a mixed A. fumigatus—S. maltophilia biofilm was validated by various analytical and quantitative approaches and the bacterial antibiosis effect on the fungus was demonstrated. The mixed biofilm model is an interesting experimentation field to evaluate efficiency of antimicrobial agents and to analyse the interactions between the biofilm and the airways epithelium.

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Section: Discussionmentioning

confidence: 99%

Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm

et al. 2016

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“…; de Rossi et al . ). The ability to switch between yeast and hyphal forms is an important facet of C. albicans virulence, with the hyphal form having key roles in the infection process.…”

Section: Dsf Family Molecules As Potential Therapeuticsmentioning

confidence: 97%

“…DSF, BDSF and CDSF can all act to inhibit the yeastto-hyphal morphological transition in C. albicans (Boon et al 2008;He et al 2010;de Rossi et al 2014). The ability to switch between yeast and hyphal forms is an important facet of C. albicans virulence, with the hyphal form having key roles in the infection process.…”

Section: Dsf Family Molecules As Potential Therapeuticsmentioning

confidence: 99%

Diffusible signal factor-dependent quorum sensing in pathogenic bacteria and its exploitation for disease control

Dow

2016

J Appl Microbiol

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Summary Cell‐to‐cell signals of the diffusible signal factor (DSF) family are cis‐2‐unsaturated fatty acids of differing chain length and branching pattern. DSF signalling has been described in diverse bacteria to include plant and human pathogens where it acts to regulate functions such as biofilm formation, antibiotic tolerance and the production of virulence factors. DSF family signals can also participate in interspecies signalling with other bacteria and interkingdom signalling such as with the yeast Candida albicans. Interference with DSF signalling may afford new opportunities for the control of bacterial disease. Such strategies will depend in part on detailed knowledge of the molecular mechanisms underlying the processes of signal synthesis, perception and turnover. Here, I review both recent progress in understanding DSF signalling at the molecular level and prospects for translating this knowledge into approaches for disease control.

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“…Bioilms created by Candida sp. yeast can reach a thickness of 25-450 μm [1,5,10,11,17]. There are three [6,11] or four [1,18] phases of Candida bioilm formation.…”

Section: Candida Dimorphism and The Bioilm Formationmentioning

confidence: 99%

Candida Biofilms: Environmental and Clinical Aspects

Targalska¹,

Kunicka-Styczyńska²

2018

The Yeast Role in Medical Applications

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Candida spp. strains are characterized by their ability to form a bioilm structure on biotic and abiotic surfaces, causing signiicant problems in many industrial branches and threatening human health. Candida bioilm is a heterogeneous, spatially well-organized structure consisting of planktonic and mycelial yeast forms which are interdependent in the quorum sensing system and surrounded by an extracellular polysaccharide substance. Bioilm-forming microorganisms are characterized by high invasiveness, the ability to cause dangerous and diicult to treat infections. Furthermore, the cells in the bioilm, compared to planktonic forms, show reduced sensitivity to chemical compounds with antifungal activity and increased survival under unfavorable environmental conditions. The chapter focuses on the emergence of antifungal resistance with the development of bioilms. The work presents the examples of antibiotic resistance of a variety of Candida, showing that a group of strains expressing intermediate sensitivity or resistance to the tested antibiotics include both clinical and food-borne isolates. Similarities in enzymatic and biochemical proiles of diferent origin isolates are discussed. A substantial heterogeneity within Candida albicans group is also underlined. Simultaneously, the incidents of biochemical proiles conformity of some clinical and food-borne isolates are presented, which may be a result of Candida transmission via food.

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Stenotrophomonas maltophilia interferes via the DSF-mediated quorum sensing system with Candida albicans filamentation and its planktonic and biofilm modes of growth

Cited by 26 publications

References 36 publications

Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm

Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm

Diffusible signal factor-dependent quorum sensing in pathogenic bacteria and its exploitation for disease control

Candida Biofilms: Environmental and Clinical Aspects

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