Stenotrophomonas nematodicola sp. nov., isolated from Caenorhabditis elegans

Wei, Xuyang; Lei, Shengwei; Deng, Yang; Zhang, Yuqin; Lin, Zhang; Niu, Qiuhong

doi:10.1007/s00203-021-02586-y

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…Stenotrophomonas and Bacillus isolated with wild South American bacterivore nematodes have also been found as part of the microbiota of nematodes elsewhere ( 6 , 7 , 31 ), thus likely making the association of these bacteria with wild worms not incidental. However, whether these isolates comprise a preferred diet in the context of a natural environment or are part of the nematode microbiota needs further assessment.…”

Section: Discussionmentioning

confidence: 99%

The Assembly of Bacteria Living in Natural Environments Shapes Neuronal Integrity and Behavioral Outputs in Caenorhabditis elegans

Urquiza-Zurich

García-Angulo

Burdisso

et al. 2023

mBio

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Do behavioral choices depend on animals’ microbiota? To answer this question, we studied how different bacterial assemblies impact the life-history traits of the bacterivore nematode C. elegans using isolated bacteria found in association with wild nematodes in Chilean soil. We identified the first isolate, Iso1, as a novel species of Stenotrophomonas and isolate Iso2 as Bacillus pumilus .

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Section: Discussionmentioning

confidence: 99%

The Assembly of Bacteria Living in Natural Environments Shapes Neuronal Integrity and Behavioral Outputs in Caenorhabditis elegans

Urquiza-Zurich

García-Angulo

Burdisso

et al. 2023

mBio

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show abstract

“…Stenotrophomonas and Bacillus isolated with wild South American bacterivore nematodes, have been also found as part of the microbiota of nematodes elsewhere (7, 6, 24), thus likely making the association of these bacteria to the wild worms not incidental. However, whether these isolates comprise a preferred diet in the context of a natural environment, needs further assessment.…”

Section: Discussionmentioning

confidence: 99%

The assembly of wild natural isolates define neuronal integrity and life history traits of co-inhabiting C. elegans

Urquiza-Zurich

García-Angulo

Burdisso

et al. 2022

Preprint

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Bacterivore nematodes are the most abundant animals in the biosphere, largely contributing to global biogeochemistry. The effect of environmental microbes as source of associated microbiota and natural diet on their life history traits of nematodes is likely to impact the general health of the biosphere. Caenorhabditis elegans is a unique model to study the behavioral and physiological outputs of different available microbial diets. Nonetheless, most studies are on monoaxenic cultures of laboratory bacteria while the effect of natural microbiota isolates has only recently started to be reported. Here, we quantified physiological, phenotypical and behavioral traits of worms feeding on two bacteria that co-isolated with wild nematodes and tested how combinations of these isolates with other bacteria affected the traits measured. These bacteria were identified as a putative novel species of Stenotrophomonas denominated Stenotrophomonas sp. Iso1 and a strain of Bacillus pumilus designated Iso2. The isolates induced distinctive behaviors and development patterns that changed in mixes of the two bacteria and/or the pathogen Salmonella enterica. Focusing on the degeneration rate of the touch circuit of C. elegans we show that B. pumilus alone is protective while the mix with Stenotrophomonas sp. is degenerative. The analysis of the metabolite content of each isolate and their combination identified NAD+ as potentially neuroprotective. In vivo supplementation shows that NAD+ restores neuroprotection to the mixes and also to individual non-protective bacteria. The results highlight the need to study the physiological effects of bacteria resembling native diets in a multicomponent scenario rather than using single isolates.

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“…The first described specie of the Stenotrophomonas genus was S. maltophilia which was a Gramnegative bacterium originally named as Pseudomonas maltophilia, and later transferred in 1993 to the new genus Stenotrophomonas, which was composed solely by S. maltophilia. In 2001 this specie was moved to the genus Xanthomonas before it was finally moved back again in 2017 to its own genus when Stenotrophomonas pictorum was identified (Ryan et al, 2009;Wei et al, 2021). Currently, Stenotrophomonas is a genus comprising at least 19 validated species (https://lpsn.dsmz.de/genus/stenotrophomonas) (Parte et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Draft genome sequence of Stenotrophomonas goyisp. nov., a novel bacterium associated with the algaChlamydomonas reinhardtii

Torres¹,

Fakhimi

Dubini³

et al. 2023

Preprint

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Stenotrophomonas goyi sp. nov. has been isolated from a contaminated algal culture (Chlamydomonas reinhardtii). Its genome has been fully sequenced (4,487,389 base pairs) and a tentative annotation is provided (4,147 genes). The genome information suggests that S. goyi sp. nov. is unable to use sulfate and nitrate as sulfur and nitrogen sources, respectively. Growth tests have confirmed the dependence of the sulfur-containing amino acids methionine and cysteine. The potential biotechnological interest of this bacteria is discussed here and in a related research paper (Fakhimi et al., 2023b).

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Stenotrophomonas nematodicola sp. nov., isolated from Caenorhabditis elegans

Cited by 7 publications

References 26 publications

The Assembly of Bacteria Living in Natural Environments Shapes Neuronal Integrity and Behavioral Outputs in Caenorhabditis elegans

The Assembly of Bacteria Living in Natural Environments Shapes Neuronal Integrity and Behavioral Outputs in Caenorhabditis elegans

The assembly of wild natural isolates define neuronal integrity and life history traits of co-inhabiting C. elegans

Draft genome sequence of Stenotrophomonas goyisp. nov., a novel bacterium associated with the algaChlamydomonas reinhardtii

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