Step Section Analysis in Routine Dermatopathology practiceA study.

Sangeetha, R.

doi:10.9790/0853-0464147

Cited by 3 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…every 50 microns) creates a step section. [1] Deeper sections are sometimes used interchangeably with serial and step sections. [2] Journal of Advanced Oral Research / May-Aug 2013 / Vol.…”

Section: Serial Sections and Step Sectionsmentioning

confidence: 99%

“…However, by using step sections it is possible to subject the residual sections for special stains [3] and if needed or can be retained for future usage. [1] Bruecks et al used the time-motion, task analysis approach to assess the technical labour costs of prospective step sections verses retrospective step sections and concluded that the use of prospective step sections is essentially cost neutral and case turn-around time is improved by 9-45%. [2] RETROSPECTIVE AND PROSPECTIVE SECTIONS Traditionally, deeper levels are obtained, at the request of the pathologist, after the original slides have been reviewed-retrospective step sections.…”

Section: Advantages Of Step Verses Serial Sectionsmentioning

confidence: 99%

“…In some laboratories step sections are prepared prior to receipt of the slides by the histopathologist-prospective step sections. [1] Situations that warrant deeper sections are following:…”

Section: Advantages Of Step Verses Serial Sectionsmentioning

confidence: 99%

“…• Initial sections when obtained are superficial and undiagnostic [2] • To identify positive areas that could contribute to a proper diagnosis and rule out certain diagnosis [2,4] • To avoid diagnostic error and subsequent malpractice claims [5] • To enhance sensitivity & diagnostic accuracy [2,6,7] • Disclosing additional pathological findings & hidden malignancies [1,2] • Orientation & plane of sectioning of the tissue specimen pose problems [8,9] labelling of sections.) [1] • Increased supply costs for staining materials, glass slides, etc., and the costs for an Increased storage volume must be considered [1] • Delay in dispatching the report [2] The studies undertaken emphasizing its importance is sparse. Currently, standards for handling small oral biopsy specimens have not been established.…”

Section: Advantages Of Step Verses Serial Sectionsmentioning

confidence: 99%

See 3 more Smart Citations

Deep Sections, a Guiding Path for Accurate Diagnosis in Histopathology - A Retrospective Study

Paul

Rao

Nagaraja

2013

Journal of Advanced Oral Research

View full text Add to dashboard Cite

“…every 50 microns) creates a step section. [1] Deeper sections are sometimes used interchangeably with serial and step sections. [2] Journal of Advanced Oral Research / May-Aug 2013 / Vol.…”

Section: Serial Sections and Step Sectionsmentioning

confidence: 99%

Section: Advantages Of Step Verses Serial Sectionsmentioning

confidence: 99%

Section: Advantages Of Step Verses Serial Sectionsmentioning

confidence: 99%

Section: Advantages Of Step Verses Serial Sectionsmentioning

confidence: 99%

See 2 more Smart Citations

Deep Sections, a Guiding Path for Accurate Diagnosis in Histopathology - A Retrospective Study

Paul

Rao

Nagaraja

2013

Journal of Advanced Oral Research

View full text Add to dashboard Cite

“…Deeper sections are named according to methodology, namely-Serial sectioning, which is defined as obtaining a continuous ribbon of sections from a paraffin block & placing all the sections on multiple slides when compared to step sections which are a form of sampling in which sections are collected at specified depths in the block. 14 In step sectioning, sections are discarded in between the sections that are mounted on the glass slide.…”

Section: Discussionmentioning

confidence: 99%

Stepping on diagnosis with step sections- An institutional experience

Kulkarni¹,

Sushmita.²,

Bijjaragi³

et al. 2020

ACHR

View full text Add to dashboard Cite

Introduction: A thorough morphological assessment plays a central role in evaluation of skin biopsies. Overlapping microscopic features pose additional diagnostic challenges in dermatopathological practice. More over skin biopsies are of small size and diagnostic findings may not be present in the initial section. Hence, step sectioning may have added value as in adjunct in contemplating the final diagnosis. Objective: The study was aimed to evaluate the diagnostic utility of step sectioning in dermatopathology. Materials and Methods: A total of 478 skin biopsies were received over a period of 2 years from January 2015 to December 2016 at the Department of Pathology, JJMMC. Two prospective step sections (A2, A3) were cut at an interval of 50µm from the original section (A1). The findings were evaluated by two pathologists independently. The results were analysed and evaluated statistically. Results: Majority of cases (n= 453, 94.7%) were diagnostic. Out of these, many cases (n= 400, 83.6%) showed no additional findings on step sections. In a few cases (n= 32, 6.7%), step section provided additional findings which contributed to the diagnosis. There were some cases (n= 14, 3%) in which findings were noted only on step section leading to the diagnosis. Some cases (n=7, 1.46%) were such that the step sections changed the diagnosis made on original section. Conclusion:Step sectioning enhances the diagnostic accuracy of morphological assessment in ermatopathology practice.

show abstract

Histopathology of Noncutaneous Small Biopsy Specimens: The Diagnostic Value of Deeper Sections

Malik¹,

Muhammad²,

Jadoon³

et al. 2022

PJMHS

View full text Add to dashboard Cite

Objective: This study aims to determine the incidence of deeper sections and the diagnostic value of these sections in non-cutaneous small biopsy specimens taken at a hospital. Study Design: Cross-sectional study Place and Duration: DHQ Teaching Hospital, Abbottabad; Jan 2022-Jun 2022. Methods: 76 patients aged 18-48 were presented in this study. Patients admitted to the oral and maxillofacial department who underwent deeper sections were included. After obtaining informed and written consent, complete demographic information was obtained. In all cases, the sites of organs and levels for seep sections were recorded. Categorical variables were assessed using mean, standard deviation, frequency, and percentages. Results: Researchers found that majority of the deeper sections, 40 (52.6%) cases were from the cervix, followed by stomach, endometrium, and colorectal. Level 4 was the most commonly performed deeper section among 18 (23.7%) cases. Even though a diagnosis could be made from the first slide, deeper levels were examined in 41 (53.9%) cases to explore other histological characteristics. This was to either increase the reliability of the diagnosis made from the first slide or to confirm that diagnosis. Of these 41 instances, 14 (34.1%) revealed the same histological characteristics in deeper sections, whereas 27 (65.9%) revealed new pathological abnormalities. Conclusion: A definitive diagnosis often has to be based on a deeper section. Therefore, regardless of the lesion size, it is advised that deeper areas be performed on samples that cannot be reliably identified on normal levels. This procedure should be standardized worldwide. Keywords: Deeper Sections, Histopathology, Biopsy, Non-cutaneous

show abstract

Step Section Analysis in Routine Dermatopathology practiceA study.

Cited by 3 publications

References 9 publications

Deep Sections, a Guiding Path for Accurate Diagnosis in Histopathology - A Retrospective Study

Deep Sections, a Guiding Path for Accurate Diagnosis in Histopathology - A Retrospective Study

Stepping on diagnosis with step sections- An institutional experience

Histopathology of Noncutaneous Small Biopsy Specimens: The Diagnostic Value of Deeper Sections

Contact Info

Product

Resources

About