2021
DOI: 10.1016/j.ymben.2021.06.004
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Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A

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Cited by 21 publications
(15 citation statements)
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“…In a recent work, the performance of P. putida KT2440 for heterologous expression of BCGs was evaluated, obtaining promising results for the production of prodigiosin and glidobactin A through genome engineering . The use of nonmodel organisms for the synthesis of this kind of compounds is an active field of research and maybe, in a near future, strain UYIF39 might be considered for such applications.…”
Section: Discussionmentioning
confidence: 99%
“…In a recent work, the performance of P. putida KT2440 for heterologous expression of BCGs was evaluated, obtaining promising results for the production of prodigiosin and glidobactin A through genome engineering . The use of nonmodel organisms for the synthesis of this kind of compounds is an active field of research and maybe, in a near future, strain UYIF39 might be considered for such applications.…”
Section: Discussionmentioning
confidence: 99%
“…Other prodigiosin‐producing strains such as S. marcescens have been reported to show still higher prodigiosin titers (more than 6 g/L), yet at the drawback of using an opportunistic pathogenic strain 4 . In addition to optimization of cultivation conditions, further genetic modification of P. putida strains has been shown to increase prodigiosin titers 50 …”
Section: Resultsmentioning
confidence: 99%
“…48 We also validated two additional plasmid-based systems from the same paper (acrylic acid and erythromycin biosensors) and a rhamnose-inducible cassette from previous work in soil bacteria, bringing the total number of tested induction systems to 15. 49 As the regulatory proteins responsive to the Marionette inducers were previously evolved to limit crosstalk, they offered a good starting set of inducible systems to onboard into new organisms using a broad host backbone. The results for the tests of these 15 induction systems are listed in Figure 3.…”
Section: Plasmid and Promoter Characterization In Nitrogen-mentioning
confidence: 99%