Stepwise pH-sensitive and biodegradable polypeptide hybrid micelles for enhanced cellular internalization and efficient nuclear drug delivery

Qu, Jing; Peng, Sixun; Wang, Rui; Yang, Shengnan; Zhou, Qing‐han; Lin, Jianwei

doi:10.1016/j.colsurfb.2019.05.071

Cited by 36 publications

(19 citation statements)

References 28 publications

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“…An Avance Neo NMR spectrometer from Bruker (Germany) was used to acquire the proton nuclear magnetic resonance ( 1 H NMR) and carbon nuclear magnetic resonance ( 13 C NMR) spectra of the P12 peptides. The NMR analytical conditions were as follows: the sample was dissolved in D 2 O.…”

Section: Methodsmentioning

confidence: 99%

“…9 In addition, micelles can significantly delay drug release, improve drug stability, and reduce toxicity under normal physiological conditions. 9,[11][12][13] Studies have shown that self-assembled peptide nanomaterials have a wide range of applications, including in gene delivery, bioimaging, drug transportation, and hemostasis. 7,[14][15][16] Since polypeptides are biodegradable in vivo and have been shown to have no toxic or immunogenic effects at lesion sites, research on drug-delivery systems based on amphiphilic polypeptides has received increasing attention.…”

Section: Introductionmentioning

confidence: 99%

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Preparation, characterization, and in vitro evaluation of amphiphilic peptide P12 and P12-DOX nanomicelles as antitumor drug carriers

Song

et al. 2020

Nanomaterials and Nanotechnology

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Polymerized polypeptide nanomicelles have attracted much attention as novel drug carriers because of their good biocompatibility and degradability. To prepare doxorubicin (DOX)-loaded nanomicelles, an amphiphilic peptide, FFHFFH-KKGRGD (P12), was synthesized by solid-phase synthesis, and the physicochemical and drug-release properties, as well as the cytotoxicity of the nanomicelles, were evaluated in vitro. The P12-DOX polymer micelles were prepared by dialysis. The morphology and particle size were characterized by transmission electron microscopy and dynamic light scattering. The critical micelle concentration (CMC) of the polymer was determined by the probe method, and the drug-release characteristics of the micelles were studied by dynamic dialysis. The cytotoxicity and uptake of the P12-DOX micelles were evaluated against mouse breast cancer cells (4T1) and human umbilical vein endothelial cells. The peptide polymer micelles containing DOX were uniformly sized and had a spherical core–shell structure with an average particle size of 128.6 nm. The CMC of the polymer was low (0.0357 mg/mL). The in vitro release of DOX from the micelles is slow and is consistent with first-order kinetics. The copolymer micelles of the P12 polypeptide and DOX can be used as nanoscale spherical carriers of hydrophobic drugs and have broad applicability.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation, characterization, and in vitro evaluation of amphiphilic peptide P12 and P12-DOX nanomicelles as antitumor drug carriers

Song

et al. 2020

Nanomaterials and Nanotechnology

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“…93 Thus, micelles based on self-assembly of poly(lysine-co-N,N-bis(acryloyl) cystamine-co-dimethylmaleic anhydride have been prepared. 94 Their surface has an overall negative charge under physiological pH, and undergoes a charge switch to positive values when exposed to the tumor environment. After internalization into the cell, endosomal escape is facilitated by a proton-sponge effect, and delivery of the cargo is triggered by reduction of the disulfide bridges of the polymer chains.…”

Section: -Dimethylmaleic Anhydride (Dmma or Dma)mentioning

confidence: 99%

Cyclic Anhydrides as Powerful Tools for Bioconjugation and Smart Delivery

Spanedda

Bourel-Bonnet

2021

Bioconjugate Chem.

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Cyclic anhydrides are potent tools for bioconjugation, therefore they are broadly used in the functionalization of biomolecules and carriers. The pH-dependent stability and reactivity, as well as the physical properties, can be tuned by the structure of the cyclic anhydride used, thus their application in smart delivery systems has become very important. This review intends to cover the last updates in the use of cyclic anhydrides as pH-sensitive linkers, their differences in reactivity and the latest applications found in bioconjugation chemistry, or chemical biology and when possible, in drug delivery.

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“…The nanomicelles fabricated from a PLL-based biodegradable polypeptide hybrid terpolymer exhibited stepwise pH- and redox response and pH-triggered charge reversal property for endosome escape [67]. Biocompatible, hemocompatible, and highly sensitive antimicrobial PLL-based peptidopolysaccharides were also reported.…”

Section: Types Of Ph-responsive Polypeptidesmentioning

confidence: 99%

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

et al. 2019

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Smart nano-carriers have attained great significance in the biomedical field due to their versatile and interesting designs with different functionalities. The initial stages of the development of nanocarriers mainly focused on the guest loading efficiency, biocompatibility of the host and the circulation time. Later the requirements of less side effects with more efficacy arose by attributing targetability and stimuli-responsive characteristics to nano-carriers along with their bio- compatibility. Researchers are utilizing many stimuli-responsive polymers for the better release of the guest molecules at the targeted sites. Among these, pH-triggered release achieves increasing importance because of the pH variation in different organ and cancer cells of acidic pH. This specific feature is utilized to release the guest molecules more precisely in the targeted site by designing polymers having specific functionality with the pH dependent morphology change characteristics. In this review, we mainly concert on the pH-responsive polypeptides and some interesting nano-carrier designs for the effective theranostic applications. Also, emphasis is made on pharmaceutical application of the different nano-carriers with respect to the organ, tissue and cellular level pH environment.

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Stepwise pH-sensitive and biodegradable polypeptide hybrid micelles for enhanced cellular internalization and efficient nuclear drug delivery

Cited by 36 publications

References 28 publications

Preparation, characterization, and in vitro evaluation of amphiphilic peptide P12 and P12-DOX nanomicelles as antitumor drug carriers

Preparation, characterization, and in vitro evaluation of amphiphilic peptide P12 and P12-DOX nanomicelles as antitumor drug carriers

Cyclic Anhydrides as Powerful Tools for Bioconjugation and Smart Delivery

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

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