Stereochemical arrangement of hydroxyl groups in sugar and polyalcohol molecules as an important factor in effective cryopreservation

Turner, Shane R.; Senaratna, Tissa; Touchell, Darren H.; Bunn, Eric; Dixon, Kingsley W.; Tan, Beng H.

doi:10.1016/s0168-9452(00)00420-9

Cited by 56 publications

(37 citation statements)

References 8 publications

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“…Glycerol appears to be essential for the cryopreservation of BY-2 cells. This result is consistent with a recent study demonstrating that glycerol is a more effective cryoprotectant than other polyalcohols and sugars (Turner et al 2001). That report proposed that the superior cryoprotective qualities of glycerol are due to its stereochemical arrangement of hydroxyl groups and small molecular size.…”

Section: Discussionsupporting

confidence: 93%

Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures

Kobayashi

Niino

Kobayashi

2005

Plant Biotechnology

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Tobacco BY-2 cells were successfully cryopreserved by a simple slow prefreezing (equilibrium freezing) method using an encapsulation technique. After the cells were immobilized in alginate gel beads, the beads were treated with cryoprotectant solution (2 M glycerol, 0.4 M sucrose) for 45 min. The beads were then transferred to a laboratory freezer at Ϫ30°C, stored for 2 h, and then immersed in liquid nitrogen. To initiate the regrowth of cells, the beads were warmed in a water bath. Following dilution of cryoprotectant solution, the beads were suspended and cultured in normal medium. With this method, suspension cell cultures were regrown within 7 days. There were no differences in the morphology or growth profiles between cryopreserved cell cultures and the original cell cultures.

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Section: Discussionsupporting

confidence: 93%

Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures

Kobayashi

Niino

Kobayashi

2005

Plant Biotechnology

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“…Other compounds which condition cold tolerance (Pontis 1989) include fructans (1-kestose) in D. antarctica, and RFOs and D-pinitol in C. quitensis plants (Piotrowicz-Cieślak et al 2005). The spatial configuration of hydroxyl groups in sugar molecules also determines their cryoprotective effects (Turner et al 2001). Hydroxyl groups have been found to exert protective effects in response to dehydration stress in developing seed embryos of many crop plant species (Peterbauer and Richter 2001;Obendorf and Górecki 2012).…”

Section: Introductionmentioning

confidence: 99%

Changes in soluble carbohydrates in polar Caryophyllaceae and Poaceae plants in response to chilling

2014

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Four species of flowering plants comprising Arctic populations of Cerastium alpinum and Poa arctica var. vivipara and indigenous Antarctic species Colobanthus quitensis and Deschampsia antarctica were investigated. Plants derived from natural origins were grown in an experimental greenhouse in Poland (53°47 0 N and 20°30 0 E latitude). Plants for experiment were collected during spring of 2010. Soluble carbohydrates in the intact shoots of C. alpinum and C. quitensis, polar plants of the family Caryophyllaceae, and D. antarctica and P. arctica var. vivipara, representatives of the family Poaceae, were analyzed by gas chromatography, and their involvement in the plants' response to chilling stress was examined. Plant tissues of the examined families growing in a greenhouse conditions (18-20°C, short day 10/14 h light/darkness) differed in the content and composition of soluble carbohydrates. In addition to common monosaccharides, myo-inositol and sucrose, Caryophyllaceae plants contained raffinose family oligosaccharides (RFOs), D-pinitol and mono-galactosyl pinitols. RFOs and D-pinitol were not detected in plants of the family Poaceae which contain 1-kestose, a specific tri-saccharide. The accumulation of significant quantities of sucrose in all investigated plants, RFOs in Caryophyllaceae plants and 1-kestose in Poaceae plants in response to chilling stress (4°C for 48 h with a long day photoperiod, 20/4 h) indicates that those compounds participate in the stress response. The common sugar accumulating in cold stress response and probably most important for chilling tolerance of four investigated plants species seems to be sucrose. On the other hand, the accumulation of above-mentioned carbohydrates during chilling stress can be a return to sugars metabolism, occurring in natural environmental conditions. No changes in D-pinitol concentrations were observed in the tissues of C. alpinum and C. quitensis plants subjected to both low and elevated temperatures, which probably rules out the protective effects of D-pinitol in response to cold stress.

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“…Thus, the total OH group concentration is of importance in the protective effect of alditols [55]. This view was supported by Turner et al [56] who emphasized the importance of the orientation of OH groups as a determining factors in effective cryopreservation. Glycerol was most effective.…”

Section: Alditol-induced Stabilization Of Proteinsmentioning

confidence: 87%

The Latest on Sugar Substitutes of the Alditol Type with Special Consideration of Erythritol and Xylitol―Rectifications and Recommendations

Mäkinen¹

2017

J Food Microbiol Saf Hyg

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Stereochemical arrangement of hydroxyl groups in sugar and polyalcohol molecules as an important factor in effective cryopreservation

Cited by 56 publications

References 8 publications

Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures

Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures

Changes in soluble carbohydrates in polar Caryophyllaceae and Poaceae plants in response to chilling

The Latest on Sugar Substitutes of the Alditol Type with Special Consideration of Erythritol and Xylitol―Rectifications and Recommendations

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