Stereologic and fine-structural studies of prostatic acinar basal cells in the dog

Ichihara, Ichiro; Kawai, Norio; Heilbronner, R.; Rohr, H.P.

doi:10.1007/bf00225417

Cited by 12 publications

(7 citation statements)

References 14 publications

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“…In addition, complex plasma membrane infoldings and interdigitations between basal and secretory cells have been found (Mao and Angrist, 1966). These ultrastructural features and the frequent close proximity of blood capillaries in the stroma to basal cells (Timms et al, 1976;Ichihara et al, 1985) support the concept of their transport role.…”

Section: The Effect Of Actinomycin D and Hydrocortisone Treatmentssupporting

confidence: 64%

“…Basal cells have also been proposed to have a role in transportation of material between secretory cells in the acinus and the extra-acinar space. Adenosine triphosphatase activity has been described on the basal cell plasma membrane (Mao and Angrist, 1966) and micropinocytotic vesicles are found on their basal surfaces (Timms et al, 1976;Ichihara et al, 1985). The pinocytotic vesicles in the rat show strong alkaline phosphatase activity (Kimura and Ichihara, 1980).…”

Section: The Effect Of Actinomycin D and Hydrocortisone Treatmentsmentioning

confidence: 99%

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Immunocytochemical localization of cathepsin D in rat ventral prostate: Evidence for castration‐induced expression of cathepsin D in basal cells

1991

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Cathepsin D (EC3.4.23.5) is an aspartyl endopeptidase involved in lysosomal proteolysis. Its functional role is uncertain. This study was undertaken to determine the cellular and subcellular distribution of cathepsin D in the normal rat ventral prostate and its possible role in the castration-induced atrophy of the gland. Cathepsin D was localized immunohistochemically to perinuclear lysosomes in secretory cells, in capillary endothelial cells, and, occasionally, in stromal cells of the untreated animal. Castration resulted in an increased number of cathepsin D-positive cells in the stroma within 24 hr. By 48 hr after castration autophagolysosomes formed in secretory cells and apoptotic bodies appeared in the epithelium. Although apoptotic bodies generally contained immunoreactive cathepsin D, a subpopulation of larger apoptotic bodies, which commonly rested on the basement membrane and contained multiple inclusions, were more variable in cathepsin D expression. The induction of cathepsin D in dendritic cells basally oriented in the epithelium was noted at 4 days of castration. These cells had a phagocytic phenotype, were distributed periodically along the basement membrane, and were not found in ductal epithelia. Treatment with actinomycin D or hydrocortisone to reduce the rate of regression of the ventral prostate blocked the appearance of these cathepsin D-positive, basally oriented epithelial cells. Our data indicate that this cathepsin D-positive, phagocytic cell differentiates from a cell resident in the prostatic epithelium. We suggest that it differentiates from basal cells in the secretory tubuloalveolar portion of the gland and that it is involved in the destruction of regressed secretory cells.

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Section: The Effect Of Actinomycin D and Hydrocortisone Treatmentssupporting

confidence: 64%

Section: The Effect Of Actinomycin D and Hydrocortisone Treatmentsmentioning

confidence: 99%

Immunocytochemical localization of cathepsin D in rat ventral prostate: Evidence for castration‐induced expression of cathepsin D in basal cells

1991

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“…The functional role of these cells is unknown, but interestingly they do not express androgen receptors and express different high molecular weight cytokeratins than the columnar cells [31,32]. They may serve as stem cells for the columnar cells, but they probably also secrete local regulatory substances [33]. It is generally assumed that prostatic cancers arise from the columnar epithelial cells and that the basal epithelial cells disappear during malignant transformation [34].…”

Section: Discussionmentioning

confidence: 99%

Differentiation‐stage specific expression of oncoprotein 18 in human and rat prostatic adenocarcinoma

et al. 1995

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Oncoprotein 18 (Op18) is an intracellular phosphoprotein that has been shown to be overexpression in a number of human malignancies. In the present report we have studied the pattern of Op18 expression on normal, hyperplastic, and malignant prostatic tissue as well as in rat prostatic tumor lines. One of the objectives of the present work was to establish whether the level of Op18 expression can be used as a prognostic marker in human prostatic adenocarcinoma. To that end, sections from normal, hyperplastic, and malignant human prostatic tissue were examined by immunohistochemistry for expression of Op18. In the normal and hyperplastic prostate, Op18 expression was observed in basal glandular epithelial cells, whereas the columnar luminal epithelial cells were not stained by the anti Op18 antibodies. In highly differentiated prostatic cancers occasional epithelial cells were stained, while in poorly differentiated tumors most of the epithelial cells contained Op18 immunoreactivity. The staining pattern was similar in the primary prostatic tumor and in the regional lymph node metastases. Most importantly, a limited survey of prostatic cancer patient samples (n = 40) showed a significant correlation between the fraction of Op18 immunoreactive cells and survival. Studies of a rat prostatic tumor model, showed that only a few cells were stained in the highly differentiated Dunning R3327PAP tumor, while most cells were stained in the anaplastic AT1 rat prostatic tumor. Interestingly, castration of rats resulted in an increased Op18 immunoreactivity, within 14 days, in the highly differentiated rat R3327PAP prostatic tumor. In conclusion, the level of Op18 expression seems to be related to cellular differentiation, histological grade, and survival in prostatic cancers. These findings show that Op18 immunoreactivity may be useful as a prognostic marker in prostatic cancer. In addition it may help in the differentiation between highly differentiated prostatic tumors and non-malignant conditions.

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“…Several studies have shown that the embryonic mesenchyme in most tissues, including the prostate [12], has an informational role for the development of the epithelium it supports [ 131. Basement membranes may also have an informational role [ 141, and, in the prostate, they are in close association with basal reserve cells [15,16]. In addition, extracellular matrices (ECM), as well as some of their components, such as collagens, fibronectin, laminin, and proteoglycans, influence not only plating efficiency and cell spreading but also cell morphology, proliferation, and differentiation [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Requirements for attachment and subsequent growth of canine prostatic epithelial cells in culture

Chevalier

Chapdelaine

1988

The Prostate

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The attachment and spreading of canine prostatic epithelial cells in primary monolayers and their subsequent proliferation were studied in Primaria and in polystyrene dishes either uncoated or coated with collagen type I, fibronectin, laminin, poly-D-lysine, or natural extracellular matrices (nECM) produced by canine prostatic epithelial or fibroblastic cells. Cells were inoculated in serum-free medium or in medium supplemented with either dialyzed fetal bovine serum (dFBS) or charcoal-treated dog serum at 10%. Dihydrotestosterone (DHT) and 5 alpha-androstane 3 alpha, 17 beta-diol (3 alpha, 17 beta-diol) at 10(-6) M or a mixture of steroids (androstenedione, testosterone, DHT, 3 alpha, 17 beta-diol, 5 alpha-androstane 3 beta, 17 beta-diol, estrone, and estradiol) were also added. Of all components and dishes tested, only dFBS and the nECM produced by prostatic epithelial cells increased cell attachment (850% and 450%, respectively). When the latter preparations were used in combination, an additive effect (1,500%) was observed, and the subsequent addition of dog serum after the attachment period yielded the highest number of growing prostatic epithelial cells. When prostatic epithelial cells were inoculated either in dishes coated with a nECM derived from prostatic fibroblasts or in presence of dog serum, a 50% inhibition in their plating efficiency was observed. The presence of collagen type I, fibronectin, laminin, or an nECM in the cultures, together with various steroids, had no effect on the cell unresponsiveness to steroids nor did it alter the mitogenic effect of dog serum. Thus, the attachment and spreading of canine prostatic epithelial cells in monolayers are mediated by nonsteroidal factors present in dFBS and in their nECM. Their presence, together with steroids, does not elicit a proliferative response to steroids nor did it increase the effect of growth-promoting factors in dog serum.

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Stereologic and fine-structural studies of prostatic acinar basal cells in the dog

Cited by 12 publications

References 14 publications

Immunocytochemical localization of cathepsin D in rat ventral prostate: Evidence for castration‐induced expression of cathepsin D in basal cells

Immunocytochemical localization of cathepsin D in rat ventral prostate: Evidence for castration‐induced expression of cathepsin D in basal cells

Differentiation‐stage specific expression of oncoprotein 18 in human and rat prostatic adenocarcinoma

Requirements for attachment and subsequent growth of canine prostatic epithelial cells in culture

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