Stereology of the myocardium inLeontopithecus(Lesson, 1840) callitrichidae – primates

Abstract

Rare morphological features of the Leontopithecus cardiovascular system have been reported in the literature. The samples analyzed in this study came from 33 specimens of Leontopithecus from the collection of the Center of Primatology of Rio de Janeiro-FEEMA (CPRJ-FEEMA). Morphometry and stereological data were obtained from all animals. Adult body weights of L. rosalia were the lowest, the greatest being those of L. chrysopygus caissara; body weights of L. chrysomelas and L. c. chrysopygus were similar and in between those of the two former species. Cardiomyocytes (left ventricular myocardium) were bigger in adults than in infants. The myocardium of L. rosalia showed focal fibrosis, fatty vacuoles, and hyalinization. In L. chrysomelas the myocardium showed areas of fibrosis and presence of mononuclear cells. Fibrosis and areas of congestion were observed in L. c. chrysopygus; areas of disorganization and vascular congestion were found in L. c. caissara. In L. rosalia infants, a greater density of vessels per myocardial area and a greater length density of vessels were observed as compared with those of L. chrysomelas. In adults, L. chrysomelas showed greater density of connective tissue in the myocardium than L. c. chrysopygus and L. c. caissara did. In L. rosalia, cardiomyocyte nuclei had a greater area density than those of the other forms of Leontopithecus. These characteristics may explain the faster development of L. rosalia infants as compared with that of L. chrysomelas and L. c. chrysopygus kept under the same handling conditions at the CPRJ-FEEMA.

Morphodynamics of Embryonic Development of Skeletal Musculature of Broiler and Layer Chickens

“…For stereological analyses of the volume density of connective tissue and the nucleocytoplasmic ratio of muscle cells, measurements were performed using the M42 testing system described elsewhere (Weibel, 1979;Pissinatti et al, 2003;Burity et al, 2004;Drabekova et al, 2005). The M42 testing system consists of 21 line segments and 42 points in a testing area.…”

Skeletal Muscle Characteristics of Broiler Chickens under Modified Incubation Factors

“…Muscle cell diameter was measured as the average of the longest lines drawn across the length and width of their cross-sections. The M42 testing system, which consists of 21 line segments and 42 points in a testing area, was used to measure connective tissue volume density and the nucleocytoplasmic ratio of muscle cells, as described elsewhere (Weibel, 1979;Pissinatti et al, 2003;Uscebrka et al, 2010). Connective tissue volume density (%) of breast muscle was calculated using the following formula:…”

Myocardial stereology in captive Callithrix kuhlii (Callitrichidae, Primates): healthy animals versus animals affected by wasting marmoset syndrome (WMS)

“…These values are similar to those obtained in the present study for Callithrix kuhlii within the same group, the "Jacchus" group. Pissinatti et al (2003) studied the myocardium of 4 taxa of Leontopithecus (L. rosalia, L. chrysomelas, L. c. chrysopygus, and L. c. caissara), and reported that V V myocyte varied from 79.6 to 88.4, while the V V connective tissue 10.2 to 17.4. These values are important for comparison with those of this study and are similar because the animals, although from different species, belong to the same family, Callitrichidae.…”