Stereoselective determination of allethrin by two-dimensional achiral/chiral liquid chromatography with ultraviolet/circular dichroism detection

Two‐dimensional liquid chromatography for direct chiral separations: a review

“…Stereoselective separation of eight stereoisomers of synthetic allethrin was possible by coupling a monolithic silica to an enantioselective OJ Daicel column though a switching valve (Mancini et al, 2004). The resolution of cis and trans diastereoisomers in the monolithic silica HPLC column was obtained using a mobile phase containing n-hexane tert-butyl methyl ether.…”

“…The pyrethroid insecticide D-allethrin was also analyzed in various commercial batches to obtain stereoisomeric composition and absolute configuration of the prevailing stereoisomers. In this achiral-chiral LC-LC coupling, cis and trans diastereomeric separation was achieved in the first dimension, while chiral separation of allethrin stereoisomers was carried out in the second one (Mancini et al, 2004).…”

HPLC quantitation of the four stereoisomers of benzoxathiepin derivatives with cellulose phenyl type chiral stationary phase and circular dichroism detection

“…Nowadays, CD spectroscopy is gaining increasing importance in pharmaceutical analysis due to the commercially available CD detectors in liquid chromatography. Several HPLC-CD applications have been reported concerning metabolism studies [12,13] and determination of absolute configuration [14][15][16]. Several authors have previously shown the interest of using CD detection in HPLC for the resolution of compounds having two [17] or three [18] pairs of enantiomers.…”

Enantioselective determination of cypermethrin in pig muscle tissue by immunoaffinity extraction and high performance liquid chromatography

“…Faraoni et al (2005) coupled an achiral HPLC column and a chiral column to investigate the stereo-selective degradation of fenvalerate in soil. Mancini et al (2004) used an achiral ⁄ chiral HPLC method to separate the eight isomers of allethrin.…”