Stereoselective determination of the active metabolites of a new anti-inflammatory agent (CS-670) in human and rat plasma using antibody-mediated extraction and high-performance liquid chromatography

The Enantioselective Immunoaffinity Extraction of an Optically Active Ibuprofen-Modified Peptide Fragment

“…Unfortunately, with this method the antibody raised may not recognize the chiral center of the ibuprofen molecule. For example, antibodies against the 2-aryl propionic acid derivatives CS-670 and laxoprofen, where the drugs were directly coupled to the carrier protein through their carboxy groups, could not recognize the asymmetric center (11,25). On the other hand, previous studies of immunogens which were prepared by condensation of drugs with a carrier protein through a bridge located far from the drug's chiral center demonstrate high recognition of the chiral center (13,26,27).…”

“…Immunoaffinity extraction with a highly specific and selective immobilized antibody is effective for the separation and extraction of trace compounds in biological fluids (11)(12)(13). The present study describes the development of a pretreatment method in which chirally specific immobilized antibodies are used to stereoselectively extract peptide fragments that are modified with optically active ibuprofen.…”

Development and validation of an LC-MS/MS method for the determination of pelubiprofen and its active metabolite, trans-alcohol, in human plasma and its application to pharmacokinetic study

“…Several analytical methods have been reported for the determination of pelubiprofen and its metabolites. However, these methods include chiral HPLC with antibody-mediated extraction [10] and GC-MS [9,11], and thus, they are not convenient for analyzing the large numbers of human samples generated during pharmacokinetic studies because of the laborious sample pretreatment [9][10][11], no human bio-sample [9], and the large amount of plasma required [10]. Furthermore, to our knowledge, no study has been previously performed on pelubiprofen and M-D simultaneous determination in human plasma http://dx.doi.org/10.1016/j.jchromb.2014.12.037 1570-0232/© 2015 Elsevier B.V. All rights reserved.…”

Sustained-release solid dispersion of pelubiprofen using the blended mixture of aminoclay and pH independent polymers: preparation and in vitro/in vivo characterization

“…PEL is effective against rheumatoid arthritis, osteoarthritis, and pain relief (Shin et al., 2011 ). Oral absorption of PEL is very fast, where T max of PEL is within 15 min in rats and 1 h in human (Takasaki et al., 1993 ; Song et al., 2016 ). In addition, PEL is metabolized rapidly to unsaturated-, cis-, and trans-alcohols after the intestinal absorption (Asami et al., 1996 ; Ryu et al., 2017 ).…”