Stereoselective oxazaborolidine–borane reduction of biphenyl alkyl ketones

Delogu, Giovanna; Fabbri, Davide; Candia, Cristina de; Patti, Angela; Pedotti, Sonia

doi:10.1016/s0957-4166(02)00210-0

Cited by 18 publications

(1 citation statement)

References 57 publications

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“…Then, a palladium‐catalyzed Heck reaction of compound 6 with n ‐butyl vinyl ether followed by the acidic hydrolysis of the resulting vinyl ether21 afforded compound 12 in 88% yield without any loss of enantioselectivity. Furthermore, the absolute configuration of the chiral axis was assigned as a R based on the comparison of its optical rotation with the value reported in the literature 22…”

Section: Methodsmentioning

confidence: 99%

Polyamide Fluorescent Probes for Visualization of Repeated DNA Sequences in Living Cells

et al. 2015

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DNA imaging in living cells usually requires transgenic approaches that modify the genome. Synthetic pyrrole-imidazole polyamides that bind specifically to the minor groove of double-stranded DNA (dsDNA) represent an attractive approach for in-cell imaging that does not necessitate changes to the genome. Nine hairpin polyamides that target mouse major satellite DNA were synthesized. Their interactions with synthetic target dsDNA fragments were studied by thermal denaturation, gel-shift electrophoresis, circular dichroism, and fluorescence spectroscopy. The polyamides had different affinities for the target DNA, and fluorescent labeling of the polyamides affected their affinity for their targets. We validated the specificity of the probes in fixed cells and provide evidence that two of the probes detect target sequences in mouse living cell lines. This study demonstrates for the first time that synthetic compounds can be used for the visualization of the nuclear substructures formed by repeated DNA sequences in living cells.

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Section: Methodsmentioning

confidence: 99%