Stereoselective toxicity mechanism of neonicotinoid dinotefuran in honeybees: New perspective from a spatial metabolomics study

Plasmonic Gold Nanoshell-Assisted Laser Desorption/Ionization Mass Spectrometry for Small-Biomolecule Analysis and Tissue Imaging

“…One issue to note is that most TG species, such as TG(48:2), TG(51:2), and TG(54:3), were found to accumulate in honeybee brain tissues, whereas some TG species, including TG(53:4) and TG(51:3), were distributed in whole-body tissues with a higher intensity in the muscle and abdomen, respectively. In addition, most PC species, such as PC(36:1), PC(34:2), and PC(34:1), mainly accumulated in the muscle region with a slight distribution in the brain and parts of the honey sac (Figure b), which is in agreement with previous reports . Various LPC and phosphatidylethanolamine (PE) species were found to exhibit similar tissue-specific distribution patterns as those for PC species.…”

“…In addition, most PC species, such as PC(36:1), PC(34:2), and PC(34:1), mainly accumulated in the muscle region with a slight distribution in the brain and parts of the honey sac (Figure 6b), which is in agreement with previous reports. 56 Various LPC and phosphatidylethanolamine (PE) species were found to exhibit similar tissue-specific distribution patterns as those for PC species. In sharp contrast, most ceramide (Cer) species, such as Cer(d36:2) and Cer(d36:3), exhibited a different accumulation pattern, which almost exclusively accumulated in the whole abdomen region (Figure S22).…”

Study on Absorption, Distribution, Metabolism, and Excretion Properties of Novel Insecticidal GABA Receptor Antagonist, Pyraquinil, in Diamondback Moth Combining MALDI Mass Spectrometry Imaging and High-Resolution Mass Spectrometry

“…27,28 Recently, our team have clarified the degradation and toxicity difference mechanism of the neonicotinoid pesticides in honeybees using MALDI-MSI. 29,30 Through these innovative applications, the use of MSI has opened new doors in toxicokinetic analysis of pesticides. 31 In the present study, we have investigated the ADME properties of pyraquinil, a novel insecticidal GABA receptor antagonist, using a combination of UHPLC-Q-Orbitrap, HPLC-MS/MS, and MALDI-MSI techniques.…”

“…However, due to the homogenization and extraction process prior to LC–MS/MS analysis, there is a complete loss of the spatiotemporal information, especially for the analytes in small insects, whose organs or tissues are difficult to identify and separate by means of manual dissection . Benefiting from the unique features of visualized, label-free and nonspecific detection, matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) has become particularly attractive for gaining the spatiotemporal information of diverse molecular species in tissue sections, without destroying the target tissues. , Now, a MALDI-MSI technique is widely used for the location of endogenous compounds and exogenous compounds in various insect tissues, such as drosophila, − mosquito, cockroach, fire ant, earthworm, fluke, − and honeybee. , Recently, our team have clarified the degradation and toxicity difference mechanism of the neonicotinoid pesticides in honeybees using MALDI-MSI. , Through these innovative applications, the use of MSI has opened new doors in toxicokinetic analysis of pesticides …”

Efficacy of Scattering Application and Paint-on Application of Insecticide in Controlling Housefly on a Broiler Farm

“…Dinotefuran has established itself as a third-generation neonicotinoid for controlling sucking pests during agricultural production in more than 20 countries (Chen et al, 2019;Yu et al, 2020). Dinotefuran acts as an agonist of nicotinic acetylcholine receptors (nAChR) in insects, which leads to irreversible binding to receptors and the resultant declining population (Belzunces et al, 2012;Zhang et al, 2022).…”