Stereospecific reduction of progesterone by Pisum sativum

Lin, Jiann‐Tsyh; Heftmanń, Erich

doi:10.1016/0031-9422(81)83020-8

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…The DET2 gene embedded in kidney 293 cells 5a-reduced testosterone, progesterone, and androstenedione . 5a-Reduction of progesterone has also been demonstrated to occur in several plants (Stohs and El-Olemy, 1972;Lin and Heftmann, 1981;Wendroth and Seitz, 1990). So, the DET2 gene seems to 5a-reduce both C 27-29 steroids and C 19/21 steroids.…”

Section: Discussionmentioning

confidence: 99%

Brassinosteroid Deficiency Due to Truncated Steroid 5α-Reductase Causes Dwarfism in thelkMutant of Pea

et al. 2004

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The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and 6-deoxotyphasterol in lk shoots were reduced 4-, 70-, and 6-fold, respectively, compared with those of the wild type. The fact that the application of brassinolide restored the growth of the mutant indicated that the dwarf mutant lk is brassinosteroid deficient. Gas chromatography-selected ion monitoring analysis of the endogenous sterols in lk shoots revealed that the levels of campestanol and sitostanol were reduced 160- and 10-fold, respectively, compared with those of wild-type plants. These data, along with metabolic studies, showed that the lk mutant has a defect in the conversion of campest-4-en-3-one to 5alpha-campestan-3-one, which is a key hydrogenation step in the synthesis of campestanol from campesterol. This defect is the same as that found in the Arabidopsis det2 mutant and the Ipomoea nil kbt mutant. The pea gene homologous to the DET2 gene, PsDET2, was cloned, and it was found that the lk mutation would result in a putative truncated PsDET2 protein. Thus it was concluded that the short stature of the lk mutant is due to a defect in the steroidal 5alpha-reductase gene. This defect was also observed in the callus induced from the lk mutant. Biosynthetic pathways involved in the conversion of campesterol to campestanol are discussed in detail.

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Section: Discussionmentioning

confidence: 99%