Sterile filtered paraffin oil supports in vitro developmental competence in bovine embryos comparable to co-culture

Tae, Jin; Kim, Eun Young; Lee, Won Don; Park, Se Pill; Lim, Jin Ho

doi:10.1007/s10815-006-9024-6

Cited by 21 publications

(21 citation statements)

References 17 publications

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“…The main function of oil coverage is the control of water evaporation to prevent the increase of medium salt concentration (Tae et al. ). So, as established by Gasperin et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes

Crocomo

Filho

Ulian

et al. 2015

Reprod Domestic Animals

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Inhibitors of cyclin-dependent kinases, as roscovitine, have been used to prevent the spontaneous resumption of meiosis in vitro and to improve the oocyte developmental competence. In this study, the interference of oil overlay on the reversible arrest capacity of roscovitine in sheep oocytes as well as its effects on cumulus expansion was evaluated. For this, cumulus-oocyte complexes (COCs) were cultured for 20 h in TCM 199 with 10% foetal bovine serum (Control) containing 75 μm roscovitine (Rosco). Subsequently, they were in vitro matured (IVM) for further 18 h in inhibitor-free medium with LH and FSH. The culture was performed in Petri dishes under mineral oil (+) or in 96 well plates without oil overlay (-) at 38.5°C and 5% CO2 . At 20 and 38 h, the cumulus expansion and nuclear maturation were evaluated under stereomicroscope and by Hoechst 33342 staining, respectively. No group presented cumulus expansion at 20 h. After additional culture with gonadotrophins, a significant rate of COCs from both Control groups (+/-) exhibited total expansion while in both Rosco groups (+/-) the partial expansion prevailed. Among the oocytes treated with roscovitine, 65.2% were kept at GV in the absence of oil overlay while 40.6% of them reached MII under oil cover (p < 0.05). This meiotic arrest was reversible, and proper meiosis progression also occurred in the Control groups (+/-). So, the culture system without oil overlay improved the meiotic inhibition promoted by roscovitine without affecting the cumulus expansion rate or the subsequent meiosis progression.

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“…The main function of oil coverage is the control of water evaporation to prevent the increase of medium salt concentration (Tae et al. ). So, as established by Gasperin et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes

Crocomo

Filho

Ulian

et al. 2015

Reprod Domestic Animals

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“…The composition of O1 and O2 are the same as in the production of cell‐free anisometric microgels. Paraffin oil is known to be noncytotoxic and is in fact used in biological systems where it can be directly in contact with cells, such as in embryo culture . In these systems, the oil is applied as a protective layer to prevent medium evaporation, maintain the pH and osmotic differences, as well as to protect from bacterial contaminations while keeping the system permeable to oxygen .…”

Section: Resultsmentioning

confidence: 99%

Cell Encapsulation in Soft, Anisometric Poly(ethylene) Glycol Microgels Using a Novel Radical‐Free Microfluidic System

Guerzoni

Rose

Gehlen

et al. 2019

Small

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Complex 3D artificial tissue constructs are extensively investigated for tissue regeneration. Frequently, materials and cells are delivered separately without benefitting from the synergistic effect of combined administration. Cell delivery inside a material construct provides the cells with a supportive environment by presenting biochemical, mechanical, and structural signals to direct cell behavior. Conversely, the cell/material interaction is poorly understood at the micron scale and new systems are required to investigate the effect of micron‐scale features on cell functionality. Consequently, cells are encapsulated in microgels to avoid diffusion limitations of nutrients and waste and facilitate analysis techniques of single or collective cells. However, up to now, the production of soft cell‐loaded microgels by microfluidics is limited to spherical microgels. Here, a novel method is presented to produce monodisperse, anisometric poly(ethylene) glycol microgels to study cells inside an anisometric architecture. These microgels can potentially direct cell growth and can be injected as rod‐shaped mini‐tissues that further assemble into organized macroscopic and macroporous structures post‐injection. Their aspect ratios are adjusted with flow parameters, while mechanical and biochemical properties are altered by modifying the precursors. Encapsulated primary fibroblasts are viable and spread and migrate across the 3D microgel structure.

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“…Despite the indications that different types of commercial oil used for IVP can greatly influence embryonic development, few studies have directly compared their effectiveness in terms of IVP outcomes. In mice (Zhu, Walker, & Maddocks, ), bovine (Tae et al., ), human (Sifer et al., ) and porcine (Martinez, Nohalez, Parrilla et al., ) IVP systems, the use of oils known as PO increased the embryo development compared to the use of oils known as MO. These observations suggest that differences in oil composition might be associated with embryo IVP results.…”

Section: Does Oil Type Influence the Ivp Outcomes?mentioning

confidence: 99%

Importance of oil overlay for production of porcine embryos in vitro

Martínez

Gil

2017

Reprod Domestic Animals

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Technologies to edit the zygote genome have revolutionized biomedical research not only for the creation of animal models for the study of human disease but also for the generation of functional human cells and tissues through interspecies blastocyst complementation technology. The pig is the ideal species for these purposes due to its great similarity in anatomy and physiology to humans. Emerging biotechnologies require the use of oocytes and/or embryos of good quality, which might be obtained using in vitro production (IVP) techniques. However, the current porcine embryo IVP systems are still suboptimal and result in low monospermic fertilization and blastocyst formation rates and poor embryo quality. During recent years, intensive investigations have been performed to evaluate the influence of specific compounds on gametes and embryos and to avoid the use of undefined supplements (serum and serum derivate) in the incubation media. However, little consideration has been given to the use of the mineral oil (MO) to overlay incubation droplets, which, albeit being a routine component of the IVP systems, is a totally undefined and thus problematic product for the safety of gametes and embryos. In this review, we provide an overview on the advantages and disadvantages of using MO to cover the incubation media. We also review one important concern in IVP laboratories: the use of oils containing undetected contamination. Finally, we discuss the effects of different types of oils on the in vitro embryo production outcomes and the transfer of compounds from oil into the culture media.

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Sterile filtered paraffin oil supports in vitro developmental competence in bovine embryos comparable to co-culture

Cited by 21 publications

References 17 publications

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes

Cell Encapsulation in Soft, Anisometric Poly(ethylene) Glycol Microgels Using a Novel Radical‐Free Microfluidic System

Importance of oil overlay for production of porcine embryos in vitro

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