Sterile filtration of liposomes: Retention of encapsulated carboxyfluorescein

Goldbach, Pierre; Brochart, Hervé; Wehrlé, Pascal; Stamm, A.

doi:10.1016/0378-5173(94)00346-7

Cited by 33 publications

(14 citation statements)

References 8 publications

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“…The increase of the ratio of amount of lipids to the amount of water droplets would increase the stability of lipid membrane and thus would improve the entrapment yield, as reported in our previous study (Sugiura et al 2008). Notably, the total lipid concentration in this sample was only about 50 lmol/l, although similar entrapment yields have been reported for large unilamellar vesicles that were prepared from lipid concentrations above 100 mmol/l by means of the conventional freezing-thawing procedure, including extrusion (Mayer et al 1986;Goldbach et al 1995). Therefore, our method is advantageous in that it features a high encapsulation efficiency of water-soluble molecules, although the obtained vesicles seem to be mostly multilamellar.…”

Section: Formation Of Giant Vesicles From W/o Emulsionsupporting

confidence: 73%

Controlled preparation of giant vesicles from uniform water droplets obtained by microchannel emulsification with bilayer-forming lipids as emulsifiers

Kuroiwa

Kiuchi

Noda

et al. 2008

Microfluid Nanofluid

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We investigated a preparation method of giant vesicles using monodisperse water-in-oil (W/O) emulsions stabilized by bilayer-forming emulsifiers. A mixture of phosphatidylcholine, cholesterol and stearylamine was used both to stabilize the water droplets formed in the emulsion and to form the vesicles. Using this lipid mixture, we obtained monodisperse W/O emulsions with mean droplet diameters of 10-40 lm and coefficients of variation as small as ca 5% by means of the microchannel (MC) emulsification technique. Utilization of an asymmetric straight-through MC array device enabled a monodisperse droplet productivity of up to 80 ml/h. The obtained water droplets were converted to giant vesicles via evaporative removal of the continuous-phase solvent followed by addition of an aqueous buffer solution. The resulting vesicles were similar in size to their starting water droplets, and a hydrophilic fluorescent marker was entrapped inside the vesicles.

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Section: Formation Of Giant Vesicles From W/o Emulsionsupporting

confidence: 73%

Controlled preparation of giant vesicles from uniform water droplets obtained by microchannel emulsification with bilayer-forming lipids as emulsifiers

Kuroiwa

Kiuchi

Noda

et al. 2008

Microfluid Nanofluid

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“…Osmotic swelling and/or incorporation of charged lipids, e.g., phosphatidylserine into bilayers are measures for increasing the aqueous volume in liposomes. 5,15 The latter is due to charge repulsion separating adjacent bilayers, resulting in increases in trapped aqueous volume.…”

Section: A Aqueous Entrapmentmentioning

confidence: 99%

Current trends in the production and biomedical applications of liposomes: a review

Uhumwangho¹,

Okor²

2009

Journal of Medicine and Biomedical Research

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A review of literature was carried out to determine methods of production of liposomes, their stability, biodistribution and their uses as drug delivery systems. The conventional method of preparing liposomes is basically for the multilamellar vesicles (MLVs). However, other methods are used to reduce the size of these MLVs to small unilamellar vesicles (SUVs) so as to increase their plasma lifetime and consequently increase the possibility of achieving greater tissue localisation. Some of these methods of size reduction are sonication and high pressure extrusion. Each of these methods has its own advantages and disadvantages. Large unilamellar vesicles (LUVs), on the other hand, are prepared mainly by detergent removal method and reverse phase extrusion technique. There are also improved pharmacokinetic properties with liposomal drugs compared to free drugs, though some formulation factors affect the release kinetics of the liposomal drugs. The review also shows that liposomes have a lot of biomedical applications and uses. They have been used in drug targeting, oral delivery of vaccines, insulins, peptides and some compounds, which are usually degraded in the gastrointestinal tract. It has also found application in topical therapy especially in the eye and lungs. Other areas of application are in cancer chemotherapy and treatment of human immunovirus (HIV) infection. The control of the stability of liposomes is an essential prerequisite for effective use as drug carriers. Leakage of the liposome is attributable mainly to differences in lamellar structure. For instance, MLVs are less prone to leakage than ULVs. The use of a combination of saturated phospholipid and cholesterol in the formulation of the liposomes has also been found to enhance stability with lower tendency to leakage.

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“…A nonionic or zwitterionic lipid is generally used as the basic lipid and to introduce surface charge other lipids are included such as stearylamine for positive charge and diacetylphosphate, phosphatidyl glycerol or phosphatidyl serine for negative charge. Incorporation of charged lipids leads to a greater overall volume for aqueous entrapment and the likelihood of aggregation reduces (Goldbach et al, 1995). However, stearylamine containing cationic liposomes have been shown to cause toxicity in rabbit (Yoshihara & Nakae, 1986).…”

Section: Liposomesmentioning

confidence: 97%

Liposomes in topical ophthalmic drug delivery: an update

et al. 2014

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Topical route of administration is the most commonly used method for the treatment of ophthalmic diseases. However, presence of several layers of permeation barriers starting from the tear film till the inner layers of cornea make it difficult to achieve the therapeutic concentrations in the target tissue within the eye. In order to circumvent these barriers and to provide sustained and targeted drug delivery, tremendous advances have been made in developing efficient and safe drug delivery systems. Liposomes due to their unique structure prove to be extremely beneficial drug carriers as they can entrap both the hydrophilic and hydrophobic drugs. The conventional liposomes had several drawbacks particularly their tendency to aggregate, the instability and leakage of entrapped drug and susceptibility to phagocytosis. Due to this reason, for a long time, liposomes as drug delivery systems did not attract much attention of researchers and clinicians. However, over recent years development of new generation liposomes has opened up new approaches for targeted and sustained drug delivery using liposomes and has rejuvenated the interest of researchers in this field. In this review we present a summary of current literature to understand the anatomical and physiological limitation in achieving adequate ocular bioavailability of topically applied drugs and utility of liposomes in overcoming these limitations. The recent developments related to new generation liposomes are discussed.

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Sterile filtration of liposomes: Retention of encapsulated carboxyfluorescein

Cited by 33 publications

References 8 publications

Controlled preparation of giant vesicles from uniform water droplets obtained by microchannel emulsification with bilayer-forming lipids as emulsifiers

Controlled preparation of giant vesicles from uniform water droplets obtained by microchannel emulsification with bilayer-forming lipids as emulsifiers

Current trends in the production and biomedical applications of liposomes: a review

Liposomes in topical ophthalmic drug delivery: an update

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